{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T15:32:14Z","timestamp":1776439934389,"version":"3.51.2"},"reference-count":62,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T00:00:00Z","timestamp":1606435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["Zoonoses research consortium PAC-Campylobacter (IP7\/ 01KI1725D) as part of the Research Network Zoonotic Infectious Diseases"],"award-info":[{"award-number":["Zoonoses research consortium PAC-Campylobacter (IP7\/ 01KI1725D) as part of the Research Network Zoonotic Infectious Diseases"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006360","name":"Bundesministerium f\u00fcr Wirtschaft und Energie","doi-asserted-by":"publisher","award":["ZIM; ZF4117904 AJ8"],"award-info":[{"award-number":["ZIM; ZF4117904 AJ8"]}],"id":[{"id":"10.13039\/501100006360","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Human Campylobacter infections are emerging worldwide and constitute significant health burdens. We recently showed that the immunopathological sequelae in Campylobacter jejuni-infected mice were due to Toll-like receptor (TLR)-4 dependent immune responses induced by bacterial lipooligosaccharide (LOS). Information regarding the molecular mechanisms underlying Campylobacter coli-host interactions are scarce, however. Therefore, we analyzed C. coli-induced campylobacteriosis in secondary abiotic IL-10\u2212\/\u2212 mice with and without TLR4. Mice were infected perorally with a human C. coli isolate or with a murine commensal Escherichia coli as apathogenic, non-invasive control. Independent from TLR4, C. coli and E. coli stably colonized the gastrointestinal tract, but only C. coli induced clinical signs of campylobacteriosis. TLR4\u2212\/\u2212 IL-10\u2212\/\u2212 mice, however, displayed less frequently fecal blood and less distinct histopathological and apoptotic sequelae in the colon versus IL-10\u2212\/\u2212 counterparts on day 28 following C. coli infection. Furthermore, C. coli-induced colonic immune cell responses were less pronounced in TLR4\u2212\/\u2212 IL-10\u2212\/\u2212 as compared to IL-10\u2212\/\u2212 mice and accompanied by lower pro-inflammatory mediator concentrations in the intestines and the liver of the former versus the latter. In conclusion, our study provides evidence that TLR4 is involved in mediating C. coli-LOS-induced immune responses in intestinal and extra-intestinal compartments during murine campylobacteriosis.<\/jats:p>","DOI":"10.3390\/microorganisms8121882","type":"journal-article","created":{"date-parts":[[2020,11,28]],"date-time":"2020-11-28T03:51:16Z","timestamp":1606535476000},"page":"1882","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Toll-Like Receptor-4 Is Involved in Mediating Intestinal and Extra-Intestinal Inflammation in Campylobacter coli-Infected Secondary Abiotic IL-10\u2212\/\u2212 Mice"],"prefix":"10.3390","volume":"8","author":[{"given":"Sigri","family":"Kl\u00f8ve","sequence":"first","affiliation":[{"name":"Institute of Micro Biology, Infectious Diseases and Immunology Charit\u00e9\u2014University Medicine Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Genger","sequence":"additional","affiliation":[{"name":"Institute of Micro Biology, Infectious Diseases and Immunology Charit\u00e9\u2014University Medicine Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis","family":"Weschka","sequence":"additional","affiliation":[{"name":"Institute of Micro Biology, Infectious Diseases and Immunology Charit\u00e9\u2014University Medicine Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraya","family":"Mousavi","sequence":"additional","affiliation":[{"name":"Institute of Micro Biology, Infectious Diseases and Immunology Charit\u00e9\u2014University Medicine Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Bereswill","sequence":"additional","affiliation":[{"name":"Institute of Micro Biology, Infectious Diseases and Immunology Charit\u00e9\u2014University Medicine Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6399-651X","authenticated-orcid":false,"given":"Markus M.","family":"Heimesaat","sequence":"additional","affiliation":[{"name":"Institute of Micro Biology, Infectious Diseases and Immunology Charit\u00e9\u2014University Medicine Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 12203 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1093\/cid\/cit555","article-title":"Preventing Campylobacter at the source: Why is it so difficult?","volume":"57","author":"Wagenaar","year":"2013","journal-title":"Clin. 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