{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T21:46:06Z","timestamp":1780695966065,"version":"3.54.1"},"reference-count":56,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T00:00:00Z","timestamp":1607558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Commission under a Marie Sklodowska-Curie Commission under a Marie Sklodowska-Curie Innovative Training Network: BtRAIN","award":["H2020-MSCA-ITN-2015, n\u00b0675619"],"award-info":[{"award-number":["H2020-MSCA-ITN-2015, n\u00b0675619"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host cells to interact with and to invade the BCSFB. To study enteroviral infection of the BCSFB, an established in vitro model based on human immortalized brain choroid plexus papilloma (HIBCPP) cells has been used. Here, we investigated the impact of E-30 infection on the protein content of the lipid rafts at the BCSFB in vitro. Mass spectrometry analysis following E-30 infection versus uninfected conditions revealed differential abundancy in proteins implicated in cellular adhesion, cytoskeleton remodeling, and endocytosis\/vesicle budding. Further, we evaluated the blocking of endocytosis via clathrin\/dynamin blocking and its consequences for E-30 induced barrier disruption. Interestingly, blocking of endocytosis had no impact on the capacity of E-30 to induce loss of barrier properties in HIBCPP cells. Altogether, these data highlight the impact of E-30 on HIBCPP cells microdomain as an important factor for host cell alteration.<\/jats:p>","DOI":"10.3390\/microorganisms8121958","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T08:59:34Z","timestamp":1607590774000},"page":"1958","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro"],"prefix":"10.3390","volume":"8","author":[{"given":"Marie","family":"Wiatr","sequence":"first","affiliation":[{"name":"Pediatric Infectious Diseases, University Children\u2019s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Staubach","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, University Hospital of Essen, University of Druisburg-Essen, 45157 Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3024-1254","authenticated-orcid":false,"given":"Ricardo","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"GenXPro GmbH, 60438 Frankfurt am Main, Germany"},{"name":"Johann Wolfgang Goethe University Frankfurt, 60438 Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolin","family":"Stump-Guthier","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, University Children\u2019s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, Tennodai, Tsukuba, Ibaraki 305-8575, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1706-0259","authenticated-orcid":false,"given":"Christian","family":"Schwerk","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, University Children\u2019s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst","family":"Schroten","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, University Children\u2019s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz-Georg","family":"Hanisch","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry II, Medical Faculty, University of Cologne, 50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henriette","family":"Rudolph","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, University Children\u2019s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Tenenbaum","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases, University Children\u2019s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1542\/peds.102.5.1126","article-title":"Clinical and economic impact of enterovirus illness in private pediatric practice","volume":"102","author":"Pichichero","year":"1998","journal-title":"Pediatrics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1097\/INF.0b013e31828f875c","article-title":"Echovirus 30 Outbreak associated with a high meningitis attack rate in Thrace, Greece","volume":"32","author":"Mantadakis","year":"2013","journal-title":"Pediatr. 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