{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:26:53Z","timestamp":1772252813939,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T00:00:00Z","timestamp":1607644800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Background: Phylogroup B2 Escherichia coli have been associated with ulcerative colitis (UC). In this study, we aimed to compare colonization with the UC-associated E. coli p19A in different mice strains, to investigate the role of alpha hemolysin in a UC mouse model. Methods: In this study, Sigirr \u2212\/\u2212 and C57BL\/6 mice were chosen, and UC was induced by adding dextran sulfate sodium (DSS) to the drinking water. The mice were pre-treated with ciprofloxacin. p19A expressing luminescence and GFP, alpha-hemolysin knock out p19A-\u0394hlyI II, and non-pathogenic lab E. coli DH10B were cultured in LB broth, and orally gavaged into the mice. Colonization with p19A WT was visualized using an in vivo imaging system. Results: p19A WT colonized the colon, ileum, Peyer\u2019s patches, liver, and spleen of infected C57BL\/6 and Sigirr \u2212\/\u2212 mice. A total of 99% of the p19A WT infected C57BL\/6 mice and 29% of the p19A WT infected Sigirr \u2212\/\u2212 mice survived to the 4th post infection day. Conclusion: UC-associated E. coli p19A WT colonized the intestines of DSS-treated mice and caused extra-intestinal infection. Hemolysin is an important factor in this pathogenesis, since isogenic hemolysin mutants did not cause the same inflammation.<\/jats:p>","DOI":"10.3390\/microorganisms8121971","type":"journal-article","created":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T20:56:57Z","timestamp":1607893017000},"page":"1971","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of \u03b1-Hemolysin Producing E. coli in Two Different Mouse Strains in a DSS Model of Inflammatory Bowel Disease"],"prefix":"10.3390","volume":"8","author":[{"given":"Hengameh Chlo\u00e9","family":"Mirsepasi-Lauridsen","sequence":"first","affiliation":[{"name":"Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark"},{"name":"Department of Biology, University of Copenhagen, 2200 Copenhagen, Denmark"},{"name":"Division of Gastroenterology, BC Children\u2019s Hospital, University of British Columbia, Vancouver, BC V6H 3N1, Canada"},{"name":"Institute of Molecular and Medical Biology, Roskilde University, 4000 Roskilde, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carsten","family":"Struve","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0531-0553","authenticated-orcid":false,"given":"Andreas Munk","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Copenhagen University Hospital Hvidovre, 2650 Hvidovre, Denmark"},{"name":"Department of Clinical Microbiology, Copenhagen University Hospital Hvidovre, 2650 Hvidovre, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen Angeliki","family":"Krogfelt","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Medical Biology, Roskilde University, 4000 Roskilde, Denmark"},{"name":"Department of Virus &amp; Microbiological Special Diagnostics, Statens Serum Institute, 2300 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1016\/S0140-6736(07)60751-X","article-title":"Inflammatory bowel disease: Clinical aspects and established and evolving therapies","volume":"369","author":"Baumgart","year":"2007","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2420","DOI":"10.1038\/ajg.2010.281","article-title":"Mucolytic Bacteria with Increased Prevalence in IBD Mucosa Augment In Vitro Utilization of Mucin by Other Bacteria","volume":"105","author":"Png","year":"2010","journal-title":"Am. 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