{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T21:53:49Z","timestamp":1783979629609,"version":"3.55.0"},"reference-count":39,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,12]],"date-time":"2020-12-12T00:00:00Z","timestamp":1607731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Committees of Region \u00d6rebro County","award":["OLL-767591"],"award-info":[{"award-number":["OLL-767591"]}]},{"name":"\u00d6sterg\u00f6tland County Council, Sweden","award":["LIO-281471"],"award-info":[{"award-number":["LIO-281471"]}]},{"name":"Nyckelfonden at O\u0308rebro University Hospital","award":["OLL-248651"],"award-info":[{"award-number":["OLL-248651"]}]},{"name":"Nyckelfonden at O\u0308rebro University Hospital","award":["OLL-595951"],"award-info":[{"award-number":["OLL-595951"]}]},{"name":"B\u00e4ckstr\u00f6mska memorial foundation and Ture and Wera Heman memorial foundation","award":["LIVFOU-258291"],"award-info":[{"award-number":["LIVFOU-258291"]}]},{"name":"Uppsala-\u00d6rebro Regional Research Council","award":["RFR-228551"],"award-info":[{"award-number":["RFR-228551"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The aim was to study alterations of bacterial communities in patients undergoing hip or knee arthroplasty to assess the impact of chlorhexidine gluconate soap decolonisation and systemic antibiotic prophylaxis. A Swedish multicentre, prospective collection of samples obtained from elective arthroplasty patients (n = 83) by swabbing anterior nares, skin sites in the groin and the site of planned surgery, before and after arthroplasty surgery, was analysed by 16S rRNA (V3-V4) gene sequencing and a complementary targeted tuf gene sequencing approach to comprehensively characterise alterations in staphylococcal communities. Significant reductions in alpha diversity was detected for both bacterial (p = 0.04) and staphylococcal (p = 0.03) groin communities after arthroplasty surgery with significant reductions in relative Corynebacterium (p = 0.001) abundance and Staphylococcus hominis (p = 0.01) relative staphylococcal abundance. In nares, significant reductions occurred for Staphylococcus hominis (p = 0.02), Staphylococcus haemolyticus (p = 0.02), and Staphylococcus pasteuri (p = 0.003) relative to other staphylococci. Staphylococcus aureus colonised 35% of anterior nares before and 26% after arthroplasty surgery. Staphylococcus epidermidis was the most abundant staphylococcal species at all sampling sites. No bacterial genus or staphylococcal species increased significantly after arthroplasty surgery. Application of a targeted tuf gene sequencing approach provided auxiliary staphylococcal community profiles and allowed species-level characterisation directly from low biomass clinical samples.<\/jats:p>","DOI":"10.3390\/microorganisms8121977","type":"journal-article","created":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T20:56:57Z","timestamp":1607893017000},"page":"1977","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1574-7396","authenticated-orcid":false,"given":"S\u00f8ren","family":"Iversen","sequence":"first","affiliation":[{"name":"Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thor Bech","family":"Johannesen","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna C\u00e4cilia","family":"Ingham","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8379-0145","authenticated-orcid":false,"given":"Sofie Marie","family":"Edslev","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9213-9274","authenticated-orcid":false,"given":"Staffan","family":"Tevell","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Centre for Clinical Research and Education, County Council of V\u00e4rmland, SE-651 82 Karlstad, Sweden"},{"name":"School of Medical Sciences, Faculty of Medicine and Health, \u00d6rebro University, SE-701 82 \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2867-1044","authenticated-orcid":false,"given":"Emeli","family":"M\u00e5nsson","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, \u00d6rebro University, SE-701 82 \u00d6rebro, Sweden"},{"name":"Centre for Clinical Research, Hospital V\u00e4stmanland, Uppsala University, Region V\u00e4stmanland, SE\u2010721 89 V\u00e4ster\u00e5s, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c5sa","family":"Nilsdotter-Augustinsson","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Department of Clinical and Biomedical Sciences, Link\u00f6ping University, SE-581 83 Norrk\u00f6ping, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5939-2932","authenticated-orcid":false,"given":"Bo","family":"S\u00f6derquist","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, \u00d6rebro University, SE-701 82 \u00d6rebro, Sweden"},{"name":"Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, \u00d6rebro University, SE-701 82 \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0321-1180","authenticated-orcid":false,"given":"Marc","family":"Stegger","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark"},{"name":"School of Medical Sciences, Faculty of Medicine and Health, \u00d6rebro University, SE-701 82 \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paal Skytt","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Bacteria, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e1006369","DOI":"10.1371\/journal.ppat.1006369","article-title":"Colonizing opportunistic pathogens (COPs): The beasts in all of us","volume":"13","author":"Price","year":"2017","journal-title":"PLoS Pathog."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1128\/CMR.00111-13","article-title":"Prosthetic joint infection","volume":"27","author":"Tande","year":"2014","journal-title":"Clin. 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