{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:10:32Z","timestamp":1790759432406,"version":"4.1.0"},"reference-count":35,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,19]],"date-time":"2021-01-19T00:00:00Z","timestamp":1611014400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BE 5748\/4-1"],"award-info":[{"award-number":["BE 5748\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01KI1728F"],"award-info":[{"award-number":["01KI1728F"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01KI1728C"],"award-info":[{"award-number":["01KI1728C"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Tick-borne encephalitis virus (TBEV), like other arthropod-transmitted viruses, depends on specific vectors to complete its enzootic cycle. It has been long known that Ixodes ricinus ticks constitute the main vector for TBEV in Europe. In contrast to the wide distribution of the TBEV vector, the occurrence of TBEV transmission is focal and often restricted to a small parcel of land, whereas surrounding areas with seemingly similar habitat parameters are free of TBEV. Thus, the question arises which factors shape this focal distribution of TBEV in the natural habitat. To shed light on factors driving TBEV-focus formation, we used tick populations from two TBEV-foci in Lower Saxony and two TBEV-foci from Bavaria with their respective virus isolates as a showcase to analyze the impact of specific virus isolate-tick population relationships. Using artificial blood feeding and field-collected nymphal ticks as experimental means, our investigation showed that the probability of getting infected with the synonymous TBEV isolate as compared to the nonsynonymous TBEV isolate was elevated but significantly higher only in one of the four TBEV foci. More obviously, median viral RNA copy numbers were significantly higher in the synonymous virus\u2013tick population pairings. These findings may present a hint for a coevolutionary adaptation of virus and tick populations.<\/jats:p>","DOI":"10.3390\/microorganisms9010196","type":"journal-article","created":{"date-parts":[[2021,1,19]],"date-time":"2021-01-19T11:39:55Z","timestamp":1611056395000},"page":"196","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The Stable Matching Problem in TBEV Enzootic Circulation: How Important Is the Perfect Tick-Virus Match?"],"prefix":"10.3390","volume":"9","author":[{"given":"Katrin","family":"Liebig","sequence":"first","affiliation":[{"name":"Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hanover, Germany"},{"name":"Research Center for Emerging Infections and Zoonosis, University of Veterinary Medicine Hanover, 30559 Hanover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Boelke","sequence":"additional","affiliation":[{"name":"Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hanover, Germany"},{"name":"Research Center for Emerging Infections and Zoonosis, University of Veterinary Medicine Hanover, 30559 Hanover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenic","family":"Grund","sequence":"additional","affiliation":[{"name":"Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hanover, Germany"},{"name":"Research Center for Emerging Infections and Zoonosis, University of Veterinary Medicine Hanover, 30559 Hanover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Schicht","sequence":"additional","affiliation":[{"name":"Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hanover, Germany"},{"name":"Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hanover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malena","family":"Bestehorn-Willmann","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Neuherbergstra\u00dfe 11, 80937 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia","family":"Chitimia-Dobler","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Neuherbergstra\u00dfe 11, 80937 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Dobler","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Neuherbergstra\u00dfe 11, 80937 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Jung","sequence":"additional","affiliation":[{"name":"Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, 30559 Hanover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie C.","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hanover, Germany"},{"name":"Research Center for Emerging Infections and Zoonosis, University of Veterinary Medicine Hanover, 30559 Hanover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,19]]},"reference":[{"key":"ref_1","unstructured":"Domingo, E., and Holland, J.J. (1994). Mutation rates and rapid evolution of RNA viruses. The Evolutionary Biology of Viruses, Raven Press."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"13910","DOI":"10.1073\/pnas.96.24.13910","article-title":"Mutation rates among RNA viruses","volume":"96","author":"Drake","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"430","DOI":"10.12998\/wjcc.v3.i5.430","article-title":"Tick-borne encephalitis: A review of epidemiology, clinical characteristics, and management","volume":"3","year":"2015","journal-title":"World J. Clin. Cases"},{"key":"ref_4","first-page":"114","article-title":"First tick-borne encephalitis in a dog resident in Northern Germany","volume":"130","author":"Hoffman","year":"2017","journal-title":"Berl. M\u00fcnch. Tier\u00e4rztl. Wochenschr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.ttbdis.2010.10.007","article-title":"Tick-borne encephalitis 2010: Epidemiology, risk areas, and virus strains in Europe and Asia\u2014An overview","volume":"2","year":"2011","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_6","first-page":"1800201","article-title":"Tick-borne encephalitis in Europe, 2012 to 2016","volume":"23","author":"Spiteri","year":"2018","journal-title":"Eurosurveillance"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Hellenbrand, W., Kreusch, T., B\u00f6hmer, M., Wagner-Wiening, C., Dobler, G., Wichmann, O., and Altmann, D. (2019). Epidemiology of Tick-Borne Encephalitis (TBE) in Germany, 2001\u20132018. Pathogens, 8.","DOI":"10.3390\/pathogens8020042"},{"key":"ref_8","first-page":"3","article-title":"Fundamentals of the theory of natural focality of transmissible human diseases","volume":"7","author":"Pavlovsky","year":"1946","journal-title":"Zh. Obshch. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Michelitsch, A., Wernike, K., Klaus, C., Dobler, G., and Beer, M. (2019). Exploring the Reservoir Hosts of Tick-Borne Encephalitis Virus. Viruses, 11.","DOI":"10.3390\/v11070669"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1099\/vir.0.035766-0","article-title":"Rate of evolution and molecular epidemiology of tick-borne encephalitis virus in Europe, including two isolations from the same focus 44 years apart","volume":"93","author":"Sironen","year":"2012","journal-title":"J. Gen. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1475-2875-4-3","article-title":"Host genotype by parasite genotype interactions underlying the resistance of anopheline mosquitoes to Plasmodium falciparum","volume":"4","author":"Lambrechts","year":"2005","journal-title":"Malar. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.virusres.2008.05.013","article-title":"Growth of tick-borne encephalitis virus (European subtype) in cell lines from vector and non-vector ticks","volume":"137","author":"Ruzek","year":"2008","journal-title":"Virus Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1093\/jmedent\/36.6.741","article-title":"Impact of Microclimate on Immature Tick-Rodent Host Interactions (Acari: Ixodidae): Implications for Parasite Transmission","volume":"36","author":"Randolph","year":"1999","journal-title":"J. Med. Entomol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.ijpara.2012.02.012","article-title":"Effects of deer density on tick infestation of rodents and the hazard of tick-borne encephalitis. I: Empirical assessment","volume":"42","author":"Cagnacci","year":"2012","journal-title":"Int. J. Parasitol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1017\/S0950268807000039","article-title":"Tick infestation on roe deer in relation to geographic and remotely sensed climatic variables in a tick-borne encephalitis endemic area","volume":"136","author":"Carpi","year":"2007","journal-title":"Epidemiol. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1007\/s10493-010-9337-0","article-title":"Tick burden on European roe deer (Capreolus capreolus)","volume":"51","author":"Vor","year":"2010","journal-title":"Exp. Appl. Acarol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Lef\u00e8vre, T., Vantaux, A., Dabir\u00e9, K.R., Mouline, K., and Cohuet, A. (2013). Non-Genetic Determinants of Mosquito Competence for Malaria Parasites. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003365"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Boelke, M., Bestehorn, M., Marchwald, B., Kubinski, M., Liebig, K., Glanz, J., Schulz, C., Dobler, G., Monazahian, M., and Becker, S.C. (2019). First Isolation and Phylogenetic Analyses of Tick-Borne Encephalitis Virus in Lower Saxony, Germany. Viruses, 11.","DOI":"10.3390\/v11050462"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-71920-z","article-title":"Tick populations from endemic and non-endemic areas in Germany show differential susceptibility to TBEV","volume":"10","author":"Liebig","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/S1386-6532(02)00168-3","article-title":"Development of a quantitative real-time RT-PCR assay with internal control for the laboratory detection of tick borne encephalitis virus (TBEV) RNA","volume":"27","author":"Schwaiger","year":"2003","journal-title":"J. Clin. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.ttbdis.2012.05.005","article-title":"Different tick-borne encephalitis virus (TBEV) prevalences in unfed versus partially engorged ixodid ticks\u2014Evidence of virus replication and changes in tick behavior","volume":"3","author":"Belova","year":"2012","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.ttbdis.2017.07.008","article-title":"Properties of the tick-borne encephalitis virus population during persistent infection of ixodid ticks and tick cell lines","volume":"8","author":"Belova","year":"2017","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ttbdis.2009.11.002","article-title":"Isolation and molecular characterization of a tick-borne encephalitis virus strain from a new tick-borne encephalitis focus with severe cases in Bavaria, Germany","volume":"1","author":"Essbauer","year":"2010","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Heinz, F.-X., and Stiasny, K. (2019). Chapter 2b: The molecular and antigenic structure of TBEV. Tick-Borne Encephalitis\u2014The Book, Global Health Press Pte Ltd.","DOI":"10.33442\/978-981-14-0914-1_2b"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Danet, L., Beauclair, G., Berthet, M., Moratorio, G., Gracias, S., Tangy, F., Choumet, V., and Jouvenet, N. (2019). Midgut barriers prevent the replication and dissemination of the yellow fever vaccine in Aedes aegypti. PLoS Negl. Trop. Dis., 13.","DOI":"10.1101\/577213"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Arias-Goeta, C., Mousson, L., Rougeon, F., and Failloux, A.-B. (2013). Dissemination and Transmission of the E1-226V Variant of Chikungunya Virus in Aedes albopictus Are Controlled at the Midgut Barrier Level. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057548"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.virol.2008.08.030","article-title":"Identification of genetic determinants of a tick-borne flavivirus associated with host-specific adaptation and pathogenicity","volume":"381","author":"Mitzel","year":"2008","journal-title":"Virology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1186\/1756-3305-7-102","article-title":"Prevalence of tick-borne encephalitis virus in Ixodes ricinus ticks in northern Europe with particular reference to Southern Sweden","volume":"7","author":"Pettersson","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-020-04146-7","article-title":"Tick-borne encephalitis virus (TBEV) prevalence in field-collected ticks (Ixodes ricinus) and phylogenetic, structural and virulence analysis in a TBE high-risk endemic area in southwestern Germany","volume":"13","author":"Ott","year":"2020","journal-title":"Parasites Vectors"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.onehlt.2016.04.001","article-title":"Culex pipiens and Culex torrentium populations from Central Europe are susceptible to West Nile virus infection","volume":"2","author":"Leggewie","year":"2016","journal-title":"One Health"},{"key":"ref_32","first-page":"1","article-title":"Vector competence of Aedes albopictus populations for chikungunya virus is shaped by their demographic history","volume":"3","author":"Marconcini","year":"2020","journal-title":"Commun. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1534\/genetics.104.035634","article-title":"Quantitative Trait Loci That Control Dengue-2 Virus Dissemination in the Mosquito Aedes aegypti","volume":"170","author":"Bennett","year":"2005","journal-title":"Genetics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"417","DOI":"10.4269\/ajtmh.18-0097","article-title":"Differential Effects of Temperature and Mosquito Genetics Determine Transmissibility of Arboviruses by Aedes aegypti in Argentina","volume":"99","author":"Ciota","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/0168-1702(89)90071-3","article-title":"Difference in vector competence of two species of sympatric ticks, Amblyomma variegatum and Rhipicephalus appendiculatus, for Dugbe virus (Nairovirus, Bunyaviridae)","volume":"14","author":"Steele","year":"1989","journal-title":"Virus Res."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/1\/196\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:12:47Z","timestamp":1760159567000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/1\/196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,19]]},"references-count":35,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,1]]}},"alternative-id":["microorganisms9010196"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms9010196","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,19]]}}}