{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:55:25Z","timestamp":1790272525514,"version":"4.1.0"},"reference-count":14,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T00:00:00Z","timestamp":1611100800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Human cryptosporidiosis remains underdiagnosed, and rapid\/accurate diagnosis is of clinical importance. Diagnosis of the Cryptosporidium oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evaluate the usefulness of a coproantigen enzyme-linked immunosorbent assay (ELISA) test in detecting Cryptosporidium spp. from fecal specimens. For this aim, we evaluated the performances of a commercial ELISA (CoproELISA Cryptosporidium kit, Savyon Diagnostics, Israel) for the detection of Cryptosporidium spp. in random clinical stool samples through a multicenter study. The sensitivity and specificity for coproantigen ELISA were 98.86% and 94.32%, respectively. The coproantigen ELISA results indicate that the simple, rapid, reliable, and standardized immunoassay test is sensitive and specific for routine diagnosis, and may be useful for large-scale epidemiological studies of cryptosporidiosis.<\/jats:p>","DOI":"10.3390\/microorganisms9020209","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T12:16:18Z","timestamp":1611144978000},"page":"209","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Multicenter Evaluation of an ELISA for the Detection of Cryptosporidium spp. Antigen in Clinical Human Stool Samples"],"prefix":"10.3390","volume":"9","author":[{"given":"Romy","family":"Razakandrainibe","sequence":"first","affiliation":[{"name":"EA ESCAPE 7510, University of Medicine Pharmacy Rouen, 76000 Rouen, France"},{"name":"CNR LE Cryptosporidiosis, Sant\u00e9 Publique France, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia","family":"M\u00e9rat","sequence":"additional","affiliation":[{"name":"Department of Parasitology\/Mycology, University Hospital of Nantes, 44093 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Kapel","sequence":"additional","affiliation":[{"name":"Department of Parasitology\/Mycology, Faculty of Pharmacy, Paris University, 75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Sautour","sequence":"additional","affiliation":[{"name":"Department of Parasitology\/Mycology, University Hospital of Dijon, 21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Guyot","sequence":"additional","affiliation":[{"name":"Department of Parasitology\/Mycology, Lille Pasteur Institute, 59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Gargala","sequence":"additional","affiliation":[{"name":"EA ESCAPE 7510, University of Medicine Pharmacy Rouen, 76000 Rouen, France"},{"name":"CNR LE Cryptosporidiosis, Sant\u00e9 Publique France, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Ballet","sequence":"additional","affiliation":[{"name":"EA ESCAPE 7510, University of Medicine Pharmacy Rouen, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5059-6402","authenticated-orcid":false,"given":"Patrice","family":"Le Pape","sequence":"additional","affiliation":[{"name":"Department of Parasitology\/Mycology, University Hospital of Nantes, 44093 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"French Cryptosporidiosis Network","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Dalle","sequence":"additional","affiliation":[{"name":"Department of Parasitology\/Mycology, University Hospital of Dijon, 21000 Dijon, France"},{"name":"CNR LE Cryptosporidiosis Collaborating Laboratory, Sant\u00e9 Publique France, 21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Favennec","sequence":"additional","affiliation":[{"name":"EA ESCAPE 7510, University of Medicine Pharmacy Rouen, 76000 Rouen, France"},{"name":"CNR LE Cryptosporidiosis, Sant\u00e9 Publique France, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1017\/S0031182014001085","article-title":"Cryptosporidium Species in Humans and Animals: Current Understanding and Research Needs","volume":"141","author":"Ryan","year":"2014","journal-title":"Parasitology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1016\/S1286-4579(02)01630-1","article-title":"Cryptosporidiosis: Epidemiology and Impact","volume":"4","author":"Dillingham","year":"2002","journal-title":"Microbes Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2","DOI":"10.4103\/tp.TP_34_17","article-title":"Laboratory Diagnosis of Cryptosporidiosis","volume":"8","author":"Khurana","year":"2018","journal-title":"Trop. Parasitol."},{"key":"ref_4","first-page":"41","article-title":"Detection of Cryptosporidium in Stool Samples of Immunocompromised Patients","volume":"7","author":"Vanathy","year":"2017","journal-title":"Trop. Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3337","DOI":"10.1128\/JCM.38.9.3337-3340.2000","article-title":"Detection of Giardia Lamblia, Entamoeba Histolytica\/Entamoeba Dispar, and Cryptosporidium Parvum Antigens in Human Fecal Specimens Using the Triage Parasite Panel Enzyme Immunoassay","volume":"38","author":"Garcia","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1128\/jcm.35.6.1526-1529.1997","article-title":"Evaluation of Nine Immunoassay Kits (Enzyme Immunoassay and Direct Fluorescence) for Detection of Giardia Lamblia and Cryptosporidium Parvum in Human Fecal Specimens","volume":"35","author":"Garcia","year":"1997","journal-title":"J. Clin. 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Bull., 15, 19642.","DOI":"10.2807\/ese.15.33.19642-en"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1128\/AEM.65.4.1578-1583.1999","article-title":"Phylogenetic Analysis of CryptosporidiumParasites Based on the Small-Subunit RRNA Gene Locus","volume":"65","author":"Xiao","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e00025-17","DOI":"10.1128\/CMR.00025-17","article-title":"Practical Guidance for Clinical Microbiology Laboratories: Laboratory Diagnosis of Parasites from the Gastrointestinal Tract","volume":"31","author":"Garcia","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_12","first-page":"792","article-title":"Laboratory Diagnosis of Cyclospora Infections","volume":"121","author":"Eberhard","year":"1997","journal-title":"Arch. Pathol. Lab. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40813-018-0097-2","article-title":"Detection of Cystoisospora Suis in Faeces of Suckling Piglets\u2014When and How? A Comparison of Methods","volume":"4","author":"Joachim","year":"2018","journal-title":"Porc. Health Manag."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1136\/bmj.327.7417.716","article-title":"Understanding Sensitivity and Specificity with the Right Side of the Brain","volume":"327","author":"Loong","year":"2003","journal-title":"BMJ"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/2\/209\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:13:03Z","timestamp":1760159583000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/2\/209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,20]]},"references-count":14,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["microorganisms9020209"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms9020209","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,20]]}}}