{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:15:09Z","timestamp":1790644509205,"version":"4.1.0"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018696","name":"R\u00e9gion Normandie","doi-asserted-by":"publisher","award":["18E01808\/18P03680"],"award-info":[{"award-number":["18E01808\/18P03680"]}],"id":[{"id":"10.13039\/501100018696","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Cryptosporidium spp. is responsible for several food and waterborne disease outbreaks worldwide. Healthier lifestyles attract consumers to eat, notably, fresh food like fruits and vegetables. The consumption of raw or under-cooked food increases the risk of foodborne transmission of Cryptosporidiosis. The assessment of the consumer\u2019s exposure to Cryptosporidium danger is crucial for public health. Still, the standardized method to detect this parasite in fresh leafy greens and berry fruits has only been available since 2016 and suffers from weaknesses. Consequently, in this study, we propose a method with minimum processing steps that combines cell culture and the quantitative PCR (CC\u2013qPCR) for detecting infectious C. parvum oocysts recovered from lamb\u2019s lettuce. This CC\u2013qPCR is a rapid and easy method that can detect up to one oocyst, whereas it is undetectable by classic qPCR.<\/jats:p>","DOI":"10.3390\/microorganisms9020215","type":"journal-article","created":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T09:49:21Z","timestamp":1611222561000},"page":"215","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Detection of Infectious Cryptosporidium parvum Oocysts from Lamb\u2019s Lettuce: CC\u2013qPCR\u2019s Intake"],"prefix":"10.3390","volume":"9","author":[{"given":"Sophie","family":"Kubina","sequence":"first","affiliation":[{"name":"ACTALIA Food Safety Department, 310 Rue Popielujko, 50 000 Saint-L\u00f4, France"},{"name":"Laboratoire de Parasitologie, EA 7510, Universit\u00e9 de Rouen Normandie, 76 000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, EA 7510, Universit\u00e9 de Rouen Normandie, 76 000 Rouen, France"},{"name":"Laboratoire de Parasitologie-Mycologie, CNR Laboratoire Expert Cryptosporidioses, Centre Hospitalier Universitaire de Rouen, 76 000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Favennec","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, EA 7510, Universit\u00e9 de Rouen Normandie, 76 000 Rouen, France"},{"name":"Laboratoire de Parasitologie-Mycologie, CNR Laboratoire Expert Cryptosporidioses, Centre Hospitalier Universitaire de Rouen, 76 000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Gargala","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, EA 7510, Universit\u00e9 de Rouen Normandie, 76 000 Rouen, France"},{"name":"Laboratoire de Parasitologie-Mycologie, CNR Laboratoire Expert Cryptosporidioses, Centre Hospitalier Universitaire de Rouen, 76 000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lique","family":"Rousseau","sequence":"additional","affiliation":[{"name":"ACTALIA Food Safety Department, 310 Rue Popielujko, 50 000 Saint-L\u00f4, France"},{"name":"Laboratoire de Parasitologie-Mycologie, EA 7510, SFR CAP-Sant\u00e9, Universit\u00e9 Reims-Champagne Ardenne, Centre Hospitalier Universitaire de Reims, 51 000 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Villena","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, EA 7510, SFR CAP-Sant\u00e9, Universit\u00e9 Reims-Champagne Ardenne, Centre Hospitalier Universitaire de Reims, 51 000 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3408-1369","authenticated-orcid":false,"given":"St\u00e9phanie","family":"La Carbona","sequence":"additional","affiliation":[{"name":"ACTALIA Food Safety Department, 310 Rue Popielujko, 50 000 Saint-L\u00f4, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romy","family":"Razakandrainibe","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, EA 7510, Universit\u00e9 de Rouen Normandie, 76 000 Rouen, France"},{"name":"Laboratoire de Parasitologie-Mycologie, CNR Laboratoire Expert Cryptosporidioses, Centre Hospitalier Universitaire de Rouen, 76 000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/j.1749-6632.1999.tb08039.x","article-title":"The threat posed by the global emergence of livestock, food-borne, and zoonotic pathogens","volume":"894","author":"Murphy","year":"1999","journal-title":"Ann. 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