{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T18:17:47Z","timestamp":1784744267950,"version":"3.55.0"},"reference-count":36,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T00:00:00Z","timestamp":1611705600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>This study compared a novel non-formaldehyde combination product developed for pathogen control in animal feed Finio (A), with a panel of three commonly used organic acid feed additive products: Fysal (B), SalCURB K2 (C) and Salgard (D). Products were evaluated for their ability to reduce Salmonella Typhimurium DT104 and avian pathogenic Escherichia coli in poultry feed. A commercial layer-hen mash was treated with each product and then mixed with feed previously contaminated (via inoculated meat and bone meal) with either Salmonella or E. coli. After 24 h at room temperature, 10 replicate samples were taken from each preparation and plate counts were performed using a selective agar. All concentrations of product A (0.5, 1.0, 1.5, 2.0 and 2.5 kg per metric tonne (MT)) plus the higher concentration of products B and D (6.0 kg MT\u22121) significantly reduced Salmonella counts compared with those in the untreated control group (p &lt; 0.05). Product C did not significantly reduce levels of Salmonella under these conditions. Because of the poor recovery of E. coli, statistical comparisons for this organism were limited in scope, but only product A at the highest concentration appeared to have eliminated it.<\/jats:p>","DOI":"10.3390\/microorganisms9020263","type":"journal-article","created":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T12:20:26Z","timestamp":1611750026000},"page":"263","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Control of Salmonella and Pathogenic E. coli Contamination of Animal Feed Using Alternatives to Formaldehyde-Based Treatments"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0829-0060","authenticated-orcid":false,"given":"Rebecca J.","family":"Gosling","sequence":"first","affiliation":[{"name":"Animal and Plant Health Agency, Woodham Lane, Addlestone, Surrey KT15 3NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Mawhinney","sequence":"additional","affiliation":[{"name":"Surveillance and Laboratory Services, Animal and Plant Health Agency, Rougham Hill, Bury St Edmunds, Suffolk IP33 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7346-2666","authenticated-orcid":false,"given":"Kurt","family":"Richardson","sequence":"additional","affiliation":[{"name":"Anitox, 7 Regent Park, Northants NN8 6GR, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8657-3007","authenticated-orcid":false,"given":"Andrew","family":"Wales","sequence":"additional","affiliation":[{"name":"Department of Pathology and Infectious Diseases, School of Veterinary Medicine, University of Surrey, Veterinary School Main Building, Daphne Jackson Road, Guildford GU2 7AL, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob","family":"Davies","sequence":"additional","affiliation":[{"name":"Animal and Plant Health Agency, Woodham Lane, Addlestone, Surrey KT15 3NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.vetmic.2013.07.003","article-title":"Salmonella contamination of cereal ingredients for animal feeds","volume":"166","author":"Davies","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0378-1135(97)00114-4","article-title":"Distribution of Salmonella contamination in ten animal feedmills","volume":"57","author":"Davies","year":"1997","journal-title":"Vet. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1111\/j.1365-2672.2010.04767.x","article-title":"Investigations into Salmonella contamination in poultry feedmills in the United Kingdom","volume":"109","author":"Davies","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"ref_4","unstructured":"EFSA (2008). Microbiological risk assessment in feedingstuffs for food-producing animals\u2014Scientific Opinion of the Panel on Biological Hazards. EFSA J., 720, 1\u201384."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Fink-Gremmels, J. (2012). Animal Feeds, Feeding Practices and Opportunities for Feed Contamination: An Introduction, in Animal Feed Contamination: Effects on Livestock and Food Safety, Woodhead Publishing Ltd.","DOI":"10.1533\/9780857093615"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2008.05.014","article-title":"Risk profiles of pork and poultry meat and risk ratings of various pathogen\/product combinations","volume":"126","author":"Mataragas","year":"2008","journal-title":"Int. J. Food Microbiol."},{"key":"ref_7","unstructured":"APHA (2018). Salmonella in Livestock Production in GB, 2017, Animal and Plant Health Agency."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1017\/S0950268800065547","article-title":"The vertical transmission of salmonellas and formic acid treatment of chicken feed. A possible strategy for control","volume":"100","author":"Humphrey","year":"1988","journal-title":"Epidemiol. Infect."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"502","DOI":"10.4315\/0362-028X-54.7.502","article-title":"A Survey of Salmonella Contamination in Modern Broiler Production","volume":"54","author":"Jones","year":"1991","journal-title":"J. Food Prot."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1136\/vr.149.8.227","article-title":"Observations on the distribution and control of Salmonella species in two integrated broiler companies","volume":"149","author":"Davies","year":"2001","journal-title":"Vet. Rec."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1086\/342885","article-title":"Bacterial contamination of animal feed and its relationship to human foodborne illness","volume":"35","author":"Crump","year":"2002","journal-title":"Clin. Infect. Dis."},{"key":"ref_12","unstructured":"ANON (2014). Salmonella in Livestock Production in Great Britian, 2013, Animal Helath and Veterinary Laboratories Agency."},{"key":"ref_13","unstructured":"APHA (2017). Salmonella in Livestock Production in GB, 2016, Animal and Plant Health Agency."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"EFSA (2018). The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017. EFSA J., 16, e05500.","DOI":"10.2903\/j.efsa.2018.5500"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.3168\/jds.S0022-0302(98)75672-3","article-title":"The occurrence and replication of Escherichia coli in cattle feeds","volume":"81","author":"Lynn","year":"1998","journal-title":"J. Dairy Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5243","DOI":"10.1128\/AEM.69.9.5243-5247.2003","article-title":"Prevalence of Escherichia coli O157 in Cattle Feeds in Midwestern Feedlots","volume":"69","author":"Dodd","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3390\/ijerph7010089","article-title":"Avian colibacillosis and salmonellosis: A closer look at epidemiology, pathogenesis, diagnosis, control and public health concerns","volume":"7","year":"2010","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.drup.2012.12.001","article-title":"Extended-spectrum cephalosporin-resistant gram-negative organisms in livestock: An emerging problem for human health?","volume":"16","author":"Seiffert","year":"2013","journal-title":"Drug Resist. Updates"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Maluta, R.P., Logue, C.M., Casas, M.R., Meng, T., Guastalli, E.A., Rojas, T.C., Montelli, A.C., Sadatsune, T., de Carvalho Ramos, M., and Nolan, L.K. (2014). Overlapped sequence types (STs) and serogroups of avian pathogenic (APEC) and human extra-intestinal pathogenic (ExPEC) Escherichia coli isolated in Brazil. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0105016"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1080\/03079450600711045","article-title":"The use of organic acids to combat Salmonella in poultry: A mechanistic explanation of the efficacy","volume":"35","author":"Russell","year":"2006","journal-title":"Avian Pathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1080\/03079457.2013.782097","article-title":"Assessment of the anti-Salmonella activity of commercial formulations of organic acid products","volume":"42","author":"Wales","year":"2013","journal-title":"Avian Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"672","DOI":"10.4315\/0362-028X.JFP-15-320","article-title":"Evaluating Chemical Mitigation of Salmonella Typhimurium ATCC 14028 in Animal Feed Ingredients","volume":"79","author":"Cochrane","year":"2016","journal-title":"J. Food Prot."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1136\/vr.123.16.416","article-title":"Control of Salmonella infections in broiler chickens by the acid treatment of their feed","volume":"123","author":"Hinton","year":"1988","journal-title":"Vet. Rec."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1080\/03079459508419071","article-title":"Studies on the use of a formic acid-propionic acid mixture (Bio-add) to control experimental Salmonella infection in broiler chickens","volume":"24","author":"Iba","year":"1995","journal-title":"Avian Pathol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/j.1365-2672.2006.03233.x","article-title":"Organic acid and formaldehyde treatment of animal feeds to control Salmonella: Efficacy and masking during culture","volume":"103","author":"Bedford","year":"2007","journal-title":"J. Appl. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1080\/19440049.2017.1363415","article-title":"Efficiency of organic acid preparations for the elimination of naturally occurring Salmonella in feed material","volume":"34","author":"Axmann","year":"2017","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"671","DOI":"10.3382\/japr.2014-00979","article-title":"Use of blends of organic acids and oregano extracts in feed and water of broiler chickens to control Salmonella Enteritidis persistence in the crop and ceca of experimentally infected birds1","volume":"23","author":"Junior","year":"2014","journal-title":"J. Appl. Poultry Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1111\/jam.12537","article-title":"Control of Salmonella Enteritidis in turkeys using organic acids and competitive exclusion product","volume":"117","author":"Milbradt","year":"2014","journal-title":"J. Appl. Microbiol."},{"key":"ref_29","first-page":"1203","article-title":"Santin E Immune response and use of organic acids in broilers challenged with Salmonella Minnesota","volume":"42","author":"Pickler","year":"2014","journal-title":"Acta Sci. Vet."},{"key":"ref_30","first-page":"195","article-title":"Effects of feed supplementation with increasing levels of organic acids on growth performance, carcass traits, gut microbiota and pH, plasma metabolites, and immune response of broilers","volume":"34","author":"Fathi","year":"2016","journal-title":"Anim. Sci. Papers Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"340","DOI":"10.3382\/japr.2014-00942","article-title":"Practical implications of plant-derived antimicrobials in poultry diets for the control of Salmonella Enteritidis","volume":"23","author":"Darre","year":"2014","journal-title":"J. Appl. Poult. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"866","DOI":"10.3389\/fmicb.2016.00866","article-title":"Antimicrobial-Resistant Escherichia coli Survived in Dust Samples for More than 20 Years","volume":"7","author":"Schulz","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"563","DOI":"10.3389\/fvets.2020.00563","article-title":"Formic Acid as an Antimicrobial for Poultry Production: A Review","volume":"7","author":"Ricke","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.4315\/0362-028X.JFP-18-124","article-title":"Adoption of Neutralizing Buffered Peptone Water Coincides with Changes in Apparent Prevalence of Salmonella and Campylobacter of Broiler Rinse Samples","volume":"81","author":"Williams","year":"2018","journal-title":"J. Food Prot."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"190","DOI":"10.3382\/japr.2012-00552","article-title":"Variations in preenrichment pH of poultry feed and feed ingredients after incubation periods up to 48 hour","volume":"22","author":"Cox","year":"2013","journal-title":"J. Appl. Poult. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"62","DOI":"10.3382\/japr\/pfv062","article-title":"Injury and death of various Salmonella serotypes due to acidic conditions","volume":"25","author":"Cox","year":"2016","journal-title":"J. Appl. Poult. 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