{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:06:33Z","timestamp":1791266793652,"version":"4.1.0"},"reference-count":55,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T00:00:00Z","timestamp":1613001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016773","name":"Shell Global Solutions International","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/100016773","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Over the last decade, metagenomic studies have revealed the impact of oil production on the microbial ecology of petroleum reservoirs. However, despite their fundamental roles in bioremediation of hydrocarbons, biocorrosion, biofouling and hydrogen sulfide production, oil field and oil production infrastructure microbiomes are poorly explored. Understanding of microbial activities within oil production facilities is therefore crucial for environmental risk mitigation, most notably during decommissioning. The analysis of the planktonic microbial community from the aqueous phase of a subsea oil-storage structure was conducted. This concrete structure was part of the production platform of the Brent oil field (North Sea), which is currently undergoing decommissioning. Quantification and sequencing of microbial 16S rRNA genes, metagenomic analysis and reconstruction of metagenome assembled genomes (MAGs) revealed a unique microbiome, strongly dominated by organisms related to Dethiosulfatibacter and Cloacimonadetes. Consistent with the hydrocarbon content in the aqueous phase of the structure, a strong potential for degradation of low molecular weight aromatic hydrocarbons was apparent in the microbial community. These degradation pathways were associated with taxonomically diverse microorganisms, including the predominant Dethiosulfatibacter and Cloacimonadetes lineages, expanding the list of potential hydrocarbon degraders. Genes associated with direct and indirect interspecies exchanges (multiheme type-C cytochromes, hydrogenases and formate\/acetate metabolism) were widespread in the community, suggesting potential syntrophic hydrocarbon degradation processes in the system. Our results illustrate the importance of genomic data for informing decommissioning strategies in marine environments and reveal that hydrocarbon-degrading community composition and metabolisms in man-made marine structures might differ markedly from natural hydrocarbon-rich marine environments.<\/jats:p>","DOI":"10.3390\/microorganisms9020356","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T18:45:00Z","timestamp":1613155500000},"page":"356","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Syntrophic Hydrocarbon Degradation in a Decommissioned Off-Shore Subsea Oil Storage Structure"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3552-8369","authenticated-orcid":false,"given":"Adrien","family":"Vigneron","sequence":"first","affiliation":[{"name":"Shell International Exploration and Production Inc., Houston, TX 77082, USA"},{"name":"School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK"},{"name":"Institut de Biologie Int\u00e9grative et des Syst\u00e8mes, Universit\u00e9 Laval, Qu\u00e9bec, QC G1S 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8628-3600","authenticated-orcid":false,"given":"Perrine","family":"Cruaud","sequence":"additional","affiliation":[{"name":"Institut de Biologie Int\u00e9grative et des Syst\u00e8mes, Universit\u00e9 Laval, Qu\u00e9bec, QC G1S 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederic","family":"Ducellier","sequence":"additional","affiliation":[{"name":"Soci\u00e9t\u00e9 des P\u00e9troles Shell, 92708 Colombes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5373-162X","authenticated-orcid":false,"given":"Ian M.","family":"Head","sequence":"additional","affiliation":[{"name":"School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5246-0568","authenticated-orcid":false,"given":"Nicolas","family":"Tsesmetzis","sequence":"additional","affiliation":[{"name":"Shell International Exploration and Production Inc., Houston, TX 77082, USA"},{"name":"School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,11]]},"reference":[{"key":"ref_1","first-page":"5","article-title":"Anaerobic Microbial Degradation of Hydrocarbons: From Enzymatic Reactions to the Environment","volume":"26","author":"Rabus","year":"2016","journal-title":"J. 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