{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T22:47:38Z","timestamp":1783118858227,"version":"3.54.6"},"reference-count":54,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T00:00:00Z","timestamp":1613088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["FISPI11\/00095 and FISPI14\/00366 FEDER FUNDING"],"award-info":[{"award-number":["FISPI11\/00095 and FISPI14\/00366 FEDER FUNDING"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["RYC-2016-19463 and RTI2018-343"],"award-info":[{"award-number":["RYC-2016-19463 and RTI2018-343"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["FPI grant to E.H.G."],"award-info":[{"award-number":["FPI grant to E.H.G."]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fondo de Investigaciones Sanitarias","award":["ISCIII-RETICRD16\/0027\/008-FEDER"],"award-info":[{"award-number":["ISCIII-RETICRD16\/0027\/008-FEDER"]}]},{"DOI":"10.13039\/100008054","name":"Fundaci\u00f3n Ram\u00f3n Areces","doi-asserted-by":"publisher","award":["CBMSO Institutional Grant"],"award-info":[{"award-number":["CBMSO Institutional Grant"]}],"id":[{"id":"10.13039\/100008054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010784","name":"Banco Santander","doi-asserted-by":"publisher","award":["CBMSO Institutiional Grant"],"award-info":[{"award-number":["CBMSO Institutiional Grant"]}],"id":[{"id":"10.13039\/100010784","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Leishmania amazonensis parasites are etiological agents of cutaneous leishmaniasis in the New World. BALB\/c mice are highly susceptible to L. amazonensis challenge due to their inability to mount parasite-dependent IFN-\u03b3-mediated responses. Here, we analyzed the capacity of a single administration of the Li\u0394HSP70-II genetically-modified attenuated L. infantum line in preventing cutaneous leishmaniasis in mice challenged with L. amazonensis virulent parasites. In previous studies, this live attenuated vaccine has demonstrated to induce long-protection against murine leishmaniasis due to Old World Leishmania species. Vaccinated mice showed a reduction in the disease evolution due to L. amazonensis challenge, namely reduction in cutaneous lesions and parasite burdens. In contrast to control animals, after the challenge, protected mice showed anti-Leishmania IgG2a circulating antibodies accompanied to the induction of Leishmania-driven specific IFN-\u03b3 systemic response. An analysis performed in the lymph node draining the site of infection revealed an increase of the parasite-specific IFN-\u03d2 production by CD4+ and CD8+ T cells and a decrease in the secretion of IL-10 against leishmanial antigens. Since the immunity caused by the inoculation of this live vaccine generates protection against different forms of murine leishmaniasis, we postulate Li\u0394HSP70-II as a candidate for the development of human vaccines.<\/jats:p>","DOI":"10.3390\/microorganisms9020363","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T18:45:00Z","timestamp":1613155500000},"page":"363","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB\/c Mice"],"prefix":"10.3390","volume":"9","author":[{"given":"Manuel","family":"Soto","sequence":"first","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM), Departamento de Biolog\u00eda Molecular, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM), Departamento de Biolog\u00eda Molecular, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0485-6127","authenticated-orcid":false,"given":"Jos\u00e9 Carlos","family":"Solana","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM), Departamento de Biolog\u00eda Molecular, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"},{"name":"WHO Collaborating Centre for Leishmaniasis, National Centre for Microbiology, Instituto de Salud Carlos III, 28220 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1415-4049","authenticated-orcid":false,"given":"Emma C. L.","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Immunology, Ophthalmology and ENT, Complutense University School of Medicine and 12 de Octubre Health Research Institute (imas12), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9018-5701","authenticated-orcid":false,"given":"Elena","family":"Hern\u00e1ndez-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Department of Immunology, Ophthalmology and ENT, Complutense University School of Medicine and 12 de Octubre Health Research Institute (imas12), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7550-0198","authenticated-orcid":false,"given":"Jos\u00e9 Mar\u00eda","family":"Requena","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM), Departamento de Biolog\u00eda Molecular, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1607-1749","authenticated-orcid":false,"given":"Salvador","family":"Iborra","sequence":"additional","affiliation":[{"name":"Department of Immunology, Ophthalmology and ENT, Complutense University School of Medicine and 12 de Octubre Health Research Institute (imas12), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Silveira, F.T. 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