{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T16:39:51Z","timestamp":1783528791987,"version":"3.55.0"},"reference-count":23,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T00:00:00Z","timestamp":1613347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["J82I15000890005"],"award-info":[{"award-number":["J82I15000890005"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Strongyloides stercoralis infection is a life-threatening neglected tropical disease. Diagnostic issues have caused an underestimation of its global burden. The choice of appropriate diagnostic tests for the screening of populations at risk of the infection, such as migrants from endemic countries, is of paramount importance. From November 2017 to July 2018, all migrants presenting to the National Institute for Health Migration and Poverty (INMP) in Rome, Italy were offered screening tests for S. stercoralis infection. The study objective was to estimate the prevalence of strongyloidiasis in the study population and the accuracy of a novel ELISA assay. The following tests were carried out at the IRCCS Sacro Cuore Don Calabria hospital in Negrar, Verona: stool microscopy, real-time PCR for S. stercoralis, in-house immunofluorescence test (IFAT), a commercial ELISA assay (Bordier ELISA), and a novel ELISA assay (Euroimmun ELISA). A latent class analysis (LCA) model set up with test results, clinical variables, and eosinophilia indicated a prevalence around 7.5%, in line with previous findings. The sensitivity and the specificity of Euroimmun ELISA were 90.6% (95% CI 80.5\u2013100) and 87.7% (95CI 84.5\u201391.0); these results indicate that the novel ELISA assay would be suitable for screening of migrants from endemic countries.<\/jats:p>","DOI":"10.3390\/microorganisms9020401","type":"journal-article","created":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T05:52:49Z","timestamp":1613368369000},"page":"401","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Prevalence of Strongyloidiasis in a Cohort of Migrants in Italy and Accuracy of a Novel ELISA Assay for S. stercoralis Infection, a Cross-Sectional Study"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0108-6822","authenticated-orcid":false,"given":"Dora","family":"Buonfrate","sequence":"first","affiliation":[{"name":"Department of Infectious Tropical Diseases and Microbiology (DITM), IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalia","family":"Marrone","sequence":"additional","affiliation":[{"name":"National Institute for Health Migration and Poverty (INMP), 00153 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2852-2287","authenticated-orcid":false,"given":"Ronaldo","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Infectious Tropical Diseases and Microbiology (DITM), IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concetta","family":"Mirisola","sequence":"additional","affiliation":[{"name":"National Institute for Health Migration and Poverty (INMP), 00153 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Ragusa","sequence":"additional","affiliation":[{"name":"Department of Infectious Tropical Diseases and Microbiology (DITM), IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Mistretta","sequence":"additional","affiliation":[{"name":"Department of Infectious Tropical Diseases and Microbiology (DITM), IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Perandin","sequence":"additional","affiliation":[{"name":"Department of Infectious Tropical Diseases and Microbiology (DITM), IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9530-8742","authenticated-orcid":false,"given":"Zeno","family":"Bisoffi","sequence":"additional","affiliation":[{"name":"Department of Infectious Tropical Diseases and Microbiology (DITM), IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar, Italy"},{"name":"Department of Diagnostics and Public Health, University of Verona, 37134 Verona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Buonfrate, D., Bisanzio, D., Giorli, G., Odermatt, P., F\u00fcrst, T., Greenaway, C., French, M., Reithinger, R., Gobbi, F., and Montresor, A. (2020). The Global Prevalence of Strongyloides stercoralis Infection. Pathogens, 9.","DOI":"10.3390\/pathogens9060468"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1017\/S0031182016000834","article-title":"Human infection with Strongyloides stercoralis and other related Strongyloides species","volume":"144","author":"Nutman","year":"2017","journal-title":"Parasitology"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Krolewiecki, A.J., Lammie, P., Jacobson, J., Gabrielli, A.F., Levecke, B., Socias, E., Arias, L.M., Sosa, N., Abraham, D., and Cimino, R. (2013). A public health response against Strongyloides stercoralis: Time to look at soil-transmitted helminthiasis in full. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002165"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/S1473-3099(19)30289-0","article-title":"Multiple-dose versus single-dose ivermectin for Strongyloides stercoralis infection (Strong Treat 1 to 4): A multicentre, open-label, phase 3, randomised controlled superiority trial","volume":"19","author":"Buonfrate","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_5","first-page":"616","article-title":"Improved diagnosis of strongyloides stercoralis by seven consecutive stool specimens","volume":"263","author":"Nielsen","year":"1987","journal-title":"Zent. Fur Bakteriol. Mikrobiol. Und Hygiene. Ser. Amedical. Microbiol. Infect. Dis. Virol. Parasitol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1590\/0037-8682-0055-2018","article-title":"Strongyloidiasis in humans: Diagnostic efficacy of four conventional methods and real-time polymerase chain reaction","volume":"51","author":"Sarmiento","year":"2018","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Buonfrate, D., Requena-Mendez, A., Angheben, A., Cinquini, M., Cruciani, M., Fittipaldo, A., Giorli, G., Gobbi, F., Piubelli, C., and Bisoffi, Z. (2018). Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006229"},{"key":"ref_8","first-page":"543","article-title":"Novel approaches to the diagnosis of Strongyloides stercoralis infection","volume":"21","author":"Buonfrate","year":"2015","journal-title":"Clin. Microbiol. Infect. Off. Publ. Eur. Soc. Clin. Microbiol. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Bisoffi, Z., Buonfrate, D., Sequi, M., Mejia, R., Cimino, R.O., Krolewiecki, A.J., Albonico, M., Gobbo, M., Bonafini, S., and Angheben, A. (2014). Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002640"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1128\/JCM.01735-06","article-title":"Use of enzyme-linked immunosorbent assay and dipstick assay for detection of Strongyloides stercoralis infection in humans","volume":"45","author":"Koelewijn","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1128\/CVI.00278-06","article-title":"Evaluation of an indirect immunofluorescence assay for strongyloidiasis as a tool for diagnosis and follow-up","volume":"14","author":"Boscolo","year":"2007","journal-title":"Clin. Vaccine Immunol. Cvi."},{"key":"ref_12","unstructured":"Oesterreich, B. (2016, January 1\u20133). Comparison of antigens from Strongyloides papillosus versus S. ratti for diagnosis of human strongyloidiasis by ELISA. Proceedings of the 2nd International Conference on Parasitology, Manchester, UK."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.trstmh.2008.12.001","article-title":"Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR","volume":"103","author":"Verweij","year":"2009","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1017\/S0031182016002559","article-title":"A retrospective study comparing agar plate culture, indirect immunofluorescence and real-time PCR for the diagnosis of Strongyloides stercoralis infection","volume":"144","author":"Buonfrate","year":"2017","journal-title":"Parasitology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"584","DOI":"10.2307\/3341848","article-title":"Applied Latent Class Analysis","volume":"28","author":"Andersen","year":"2003","journal-title":"Can. J. Sociol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e236","DOI":"10.1016\/S2214-109X(18)30490-X","article-title":"Prevalence of strongyloidiasis and schistosomiasis among migrants: A systematic review and meta-analysis","volume":"7","author":"Asundi","year":"2019","journal-title":"Lancet Glob. Health"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Martinez-P\u00e9rez, A., Soriano-P\u00e9rez, M.J., Salvador, F., Gomez-Junyent, J., Villar-Garcia, J., Santin, M., Mu\u00f1oz, C., Gonz\u00e1lez-Cord\u00f3n, A., Salas-Coronas, J., and Sulleiro, E. (2020). Clinical Features Associated with Strongyloidiasis in Migrants and the Potential Impact of Immunosuppression: A Case Control Study. Pathogens, 9.","DOI":"10.3390\/pathogens9060507"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"428","DOI":"10.4269\/ajtmh.19-0087","article-title":"Clinical and Diagnostic Features of 413 Patients Treated for Imported Strongyloidiasis at the Hospital for Tropical Diseases, London","volume":"101","author":"Ming","year":"2019","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.4269\/ajtmh.18-0895","article-title":"Morbidity Associated with Chronic Strongyloides stercoralis Infection: A Systematic Review and Meta-Analysis","volume":"100","author":"Tamarozzi","year":"2019","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_20","first-page":"17-00527","article-title":"Extended screening for infectious diseases among newly-arrived asylum seekers from Africa and Asia, Verona province, Italy, April 2014 to June 2015","volume":"23","author":"Buonfrate","year":"2018","journal-title":"Euro Surveill. Bull. Eur. Sur Les Mal. Transm. Eur. Commun. Dis. Bull."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1186\/s13071-016-1912-8","article-title":"High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: The role of intensive diagnostic work-up","volume":"9","author":"Amor","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.actatropica.2015.07.019","article-title":"Real-time PCR for detection of Strongyloides stercoralis in human stool samples from C\u00f4te d\u2019Ivoire: Diagnostic accuracy, inter-laboratory comparison and patterns of hookworm co-infection","volume":"150","author":"Becker","year":"2015","journal-title":"Acta Trop."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Warnecke, J.M. (2019, January 28\u201329). Sensitive and specific ELISA for the serological diagnosis of Strongyloides infections. Proceedings of the 6th International Conference on Tropical Medicine and Infectious Diseases, Barcelona, Spain.","DOI":"10.4172\/2329-891X-C1-001"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/2\/401\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:24:25Z","timestamp":1760160265000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/2\/401"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,15]]},"references-count":23,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["microorganisms9020401"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms9020401","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,15]]}}}