{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:57:47Z","timestamp":1792652267535,"version":"4.5.0"},"reference-count":78,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T00:00:00Z","timestamp":1617926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Pseudomonas aeruginosa has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of P. aeruginosa recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among P. aeruginosa isolates. The drug susceptibility profiles of P. aeruginosa for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (mcr-1) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (blaOXA-23 and blaOXA-51) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of blaOXA-23 and blaOXA-51 genes in P. aeruginosa.<\/jats:p>","DOI":"10.3390\/microorganisms9040786","type":"journal-article","created":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T10:05:21Z","timestamp":1617962721000},"page":"786","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Molecular Detection of Drug-Resistance Genes of blaOXA-23-blaOXA-51 and mcr-1 in Clinical Isolates of Pseudomonas aeruginosa"],"prefix":"10.3390","volume":"9","author":[{"given":"Fabiana","family":"Nitz","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Microrganismos Patog\u00eanicos, Universidade Ceuma, S\u00e3o Lu\u00eds 65075-120, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna Oliveira","family":"de Melo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Microrganismos Patog\u00eanicos, Universidade Ceuma, S\u00e3o Lu\u00eds 65075-120, Brazil"},{"name":"Laborat\u00f3rio Cedro, S\u00e3o Lu\u00eds 65020-570, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4206-0904","authenticated-orcid":false,"given":"Lu\u00eds Cl\u00e1udio Nascimento","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patogenicidade Microbiana, Universidade Ceuma, S\u00e3o Lu\u00eds 65075-120, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"de Souza Monteiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Aplicada, Universidade Ceuma, S\u00e3o Lu\u00eds 65075-120, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirlei Garcia","family":"Marques","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Cedro, S\u00e3o Lu\u00eds 65020-570, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2868-1314","authenticated-orcid":false,"given":"Val\u00e9rio","family":"Monteiro-Neto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Microrganismos Patog\u00eanicos, Universidade Ceuma, S\u00e3o Lu\u00eds 65075-120, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Universidade Federal do Maranh\u00e3o, S\u00e3o Lu\u00eds 65085-582, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosimary","family":"de Jesus Gomes Turri","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal do Maranh\u00e3o, S\u00e3o Lu\u00eds 65080-805, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Dantas Silva","family":"Junior","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Microrganismos Patog\u00eanicos, Universidade Ceuma, S\u00e3o Lu\u00eds 65075-120, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Cristina Ribeiro","family":"Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Cedro, S\u00e3o Lu\u00eds 65020-570, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hil\u00e1rio Jos\u00e9 Cardoso","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Microrganismos Patog\u00eanicos, Universidade Ceuma, S\u00e3o Lu\u00eds 65075-120, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrielle","family":"Zagmignan","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patogenicidade Microbiana, Universidade Ceuma, S\u00e3o Lu\u00eds 65075-120, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Azevedo Feitosa","family":"Ferro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Microrganismos Patog\u00eanicos, Universidade Ceuma, S\u00e3o Lu\u00eds 65075-120, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5738-3952","authenticated-orcid":false,"given":"Maria Rosa Quaresma","family":"Bomfim","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular de Microrganismos Patog\u00eanicos, Universidade Ceuma, S\u00e3o Lu\u00eds 65075-120, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e15722","DOI":"10.5812\/ircmj.15722","article-title":"Virulence Gene Profiles of Multidrug-Resistant Pseudomonas aeruginosa Isolated from Iranian Hospital Infections","volume":"16","author":"Fazeli","year":"2014","journal-title":"Iran. 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