{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:12:28Z","timestamp":1789582348071,"version":"build-2803163510"},"reference-count":116,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T00:00:00Z","timestamp":1618185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Youth and Sports of the Czech Republic","award":["LTAUSA17165"],"award-info":[{"award-number":["LTAUSA17165"]}]},{"name":"Grant Agency of the University of South Bohemia","award":["028\/2019\/Z"],"award-info":[{"award-number":["028\/2019\/Z"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31820103014"],"award-info":[{"award-number":["31820103014"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Cryptosporidium spp., common parasites of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutrias (Myocastor coypus). A total of 150 faecal samples of feral nutria were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci. Molecular analyses revealed the presence of C. parvum (n = 1), C. ubiquitum subtype family XIId (n = 5) and Cryptosporidium myocastoris n. sp. XXIIa (n = 2), and XXIIb (n = 3). Only nutrias positive for C. myocastoris shed microscopically detectable oocysts, which measured 4.8\u20135.2 \u00d7 4.7\u20135.0 \u00b5m, and oocysts were infectious for experimentally infected nutrias with a prepatent period of 5\u20136 days, although not for mice, gerbils, or chickens. The infection was localised in jejunum and ileum without observable macroscopic changes. The microvilli adjacent to attached stages responded by elongating. Clinical signs were not observed in naturally or experimentally infected nutrias. Phylogenetic analyses at SSU, actin, and HSP70 loci demonstrated that C. myocastoris n. sp. is distinct from other valid Cryptosporidium species.<\/jats:p>","DOI":"10.3390\/microorganisms9040813","type":"journal-article","created":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T21:47:33Z","timestamp":1618264053000},"page":"813","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus)"],"prefix":"10.3390","volume":"9","author":[{"given":"Jana","family":"Je\u017ekov\u00e1","sequence":"first","affiliation":[{"name":"Faculty of Agriculture, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, Studentsk\u00e1 1668, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zlata","family":"Limpouchov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitka","family":"Prediger","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, Studentsk\u00e1 1668, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikola","family":"Holubov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, Studentsk\u00e1 1668, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"},{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohumil","family":"Sak","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Kone\u010dn\u00fd","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, Studentsk\u00e1 1668, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Kv\u011bto\u0148ov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenka","family":"Hl\u00e1skov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9662-9724","authenticated-orcid":false,"given":"Michael","family":"Rost","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, Studentsk\u00e1 1668, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"McEvoy","sequence":"additional","affiliation":[{"name":"Microbiological Sciences Department, North Dakota State University, 1523 Centennial Blvd, Van Es Hall, Fargo, ND 58102, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Du\u0161an","family":"Rajsk\u00fd","sequence":"additional","affiliation":[{"name":"Faculty of Forestry, Technical University in Zvolen, 960 01 Zvolen, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8380-1246","authenticated-orcid":false,"given":"Yaoyu","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kv\u00e1\u010d","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, Studentsk\u00e1 1668, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"},{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Brani\u0161ovsk\u00e1 31, 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.exppara.2009.03.005","article-title":"Taxonomy and species delimitation in Cryptosporidium","volume":"124","author":"Fayer","year":"2010","journal-title":"Exp. 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