{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T19:24:09Z","timestamp":1792265049496,"version":"4.4.0"},"reference-count":55,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,13]],"date-time":"2021-04-13T00:00:00Z","timestamp":1618272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Lactobacilli species are an effective biotherapeutic alternative against bacterial infections and intestinal inflammatory disorders. However, it is important to evaluate their beneficial properties, before considering them as probiotics for medical use. In this study we evaluated some probiotic properties of Lactobacillus rhamnosus GG, Lactobacillus rhamnosus KLSD, Lactobacillus helveticus IMAU70129, and Lactobacillus casei IMAU60214 previously isolated from dairy products and as control Lactobacillus casei Shirota. Experimental evaluations revealed that all strains expressed hydrophobicity (25\u201340%), auto-aggregation (55\u201360%), NaCl tolerance (1\u20134%), adhesion to Caco-2 cells (25\u201333%), partial inhibition on adherence of Escherichia coli ATCC 35218, Salmonella Typhimurium ATCC 14028, and Staphylococcus aureus ATCC 23219. Cell-free supernatants (CFS) of Lactobacilli also inhibit growth of these pathogens. In immunomodulatory properties a reduction of interleukin-8 (IL-8) and nitric oxide (NO) release was observed in assays with Caco-2 cells stimulated with interleukin-1\u03b2 (1 ng\/mL), or lipopolysaccharide (0.1 \u00b5g\/mL). On the other hand, the damage induced to Caco-2 cells with sodium dodecyl sulfate (SDS) was attenuated when the cultured cells were pretreated with L. rhamnosus KLDS, L. helveticus IMAU70129 and L. casei IMAU60214. These Lactobacilli possess probiotic properties determined by both an antagonistic activity on pathogenic bacteria and reduction in the inflammatory response of cells treated with SDS, a pro-inflammatory stimulant.<\/jats:p>","DOI":"10.3390\/microorganisms9040825","type":"journal-article","created":{"date-parts":[[2021,4,13]],"date-time":"2021-04-13T10:58:07Z","timestamp":1618311487000},"page":"825","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Probiotic Properties and Immunomodulatory Activity of Lactobacillus Strains Isolated from Dairy Products"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7204-1421","authenticated-orcid":false,"given":"Luz Mar\u00eda","family":"Rocha-Ram\u00edrez","sequence":"first","affiliation":[{"name":"Unidad de Investigaci\u00f3n en Enfermedades Infecciosas, Hospital Infantil de M\u00e9xico Federico G\u00f3mez, Secretaria de Salud, Dr. M\u00e1rquez No. 162, Col. Doctores, Delegaci\u00f3n Cuauht\u00e9moc, Ciudad de M\u00e9xico 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4708-8807","authenticated-orcid":false,"given":"Ulises","family":"Hern\u00e1ndez-Chi\u00f1as","sequence":"additional","affiliation":[{"name":"Unidad Perif\u00e9rica de Investigaci\u00f3n B\u00e1sica y Cl\u00ednica en Enfermedades Infecciosas, Laboratorio de Patogenicidad Bacteriana, Departamento de Salud P\u00fablica\/Divisi\u00f3n de Investigaci\u00f3n, Facultad de Medicina Universidad Nacional Aut\u00f3noma de M\u00e9xico, Unidad de Hemato-Oncolog\u00eda e Investigaci\u00f3n, Hospital Infantil de M\u00e9xico Federico G\u00f3mez\/Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Dr. M\u00e1rquez 162, Col. Doctores, Delegaci\u00f3n Cuauht\u00e9moc, Ciudad de M\u00e9xico 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia Selene","family":"Moreno-Guerrero","sequence":"additional","affiliation":[{"name":"Departamento de Hemato-Oncolog\u00eda, Hospital Infantil de M\u00e9xico Federico G\u00f3mez. Dr. M\u00e1rquez No. 162, Col. Doctores, Delegaci\u00f3n Cuauht\u00e9moc, Ciudad de M\u00e9xico 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo","family":"Ram\u00edrez-Pacheco","sequence":"additional","affiliation":[{"name":"Departamento de Hemato-Oncolog\u00eda, Hospital Infantil de M\u00e9xico Federico G\u00f3mez. Dr. M\u00e1rquez No. 162, Col. Doctores, Delegaci\u00f3n Cuauht\u00e9moc, Ciudad de M\u00e9xico 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7670-1960","authenticated-orcid":false,"given":"Carlos A.","family":"Eslava","sequence":"additional","affiliation":[{"name":"Unidad Perif\u00e9rica de Investigaci\u00f3n B\u00e1sica y Cl\u00ednica en Enfermedades Infecciosas, Laboratorio de Patogenicidad Bacteriana, Departamento de Salud P\u00fablica\/Divisi\u00f3n de Investigaci\u00f3n, Facultad de Medicina Universidad Nacional Aut\u00f3noma de M\u00e9xico, Unidad de Hemato-Oncolog\u00eda e Investigaci\u00f3n, Hospital Infantil de M\u00e9xico Federico G\u00f3mez\/Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Dr. M\u00e1rquez 162, Col. Doctores, Delegaci\u00f3n Cuauht\u00e9moc, Ciudad de M\u00e9xico 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1126\/science.1058709","article-title":"Commensal host-bacterial relationships in the gut","volume":"292","author":"Hooper","year":"2001","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1152\/physrev.00045.2009","article-title":"Gut microbiota in health and disease","volume":"90","author":"Sekirov","year":"2010","journal-title":"Physiol. 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