{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:10:05Z","timestamp":1785777005527,"version":"3.56.0"},"reference-count":54,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,14]],"date-time":"2021-05-14T00:00:00Z","timestamp":1620950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Agave species are a source of diverse products for human use, such as food, fiber, and beverages, which include mezcal, a distilled beverage produced by spontaneous fermentation. Agave is an excellent source of high amounts of sugars, minerals, and phenolic compounds, which favor the growth of lactic acid bacteria (LAB) and yeast communities. In this work, 20 promising LAB strains with probiotic characteristics were isolated from the agave fermentation stage in mezcal production. The strains belonged to Lactobacillus plantarum (15), Lactobacillus rhamnosus (2), Enterococcus faecium (2), and Lactococcus lactis (1). These isolates were characterized for their resistance under gastrointestinal conditions, such as lysozyme, acid pH, and bile salts. In addition, the adherence of these LABs to human intestinal epithelial cells (Caco-2 and HT-29 cells) was tested in vitro and their antioxidant and immunomodulatory profile was determined using cellular models. Lactobacillus rhamnosus LM07 and Lactobacillus plantarum LM17 and LM19 strains were selected for their antioxidant properties, and their capacities in an oxidative stress model in intestinal epithelial cells IECs (Caco-2 and HT-29 cells) in the presence of hydrogen peroxide were evaluated. Interestingly, Lactobacillus rhamnosus LM07 and Lactobacillus plantarum LM17 and LM19 strains showed anti-inflammatory properties in TNF-\u03b1-stimulated HT-29 cells. Subsequently, bacterial strains exhibiting antioxidant and anti-inflammatory properties were tested in vivo in a mouse model with dinitrobenzene sulfonic acid (DNBS)-induced chronic colitis. Weight loss, intestinal permeability, and cytokine profiles were measured in mice as indicators of inflammation. One of the selected strains, Lactobacillus plantarum LM17, improved the health of the mice, as observed by reduced weight loss, and significantly decreased intestinal permeability. Altogether, our results demonstrate the potential of LAB (and lactobacilli in particular) isolated from the agave fermentation stage in mezcal production. Lactobacillus rhamnosus LM07 and Lactobacillus plantarum LM17 strains represent potential candidates for developing new probiotic supplements to treat inflammatory bowel disease (IBD).<\/jats:p>","DOI":"10.3390\/microorganisms9051063","type":"journal-article","created":{"date-parts":[[2021,5,14]],"date-time":"2021-05-14T10:54:22Z","timestamp":1620989662000},"page":"1063","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Antioxidant and Anti-Inflammatory Properties of Probiotic Candidate Strains Isolated during Fermentation of Agave (Agave angustifolia Haw)"],"prefix":"10.3390","volume":"9","author":[{"given":"Natalia C.","family":"Hern\u00e1ndez-Delgado","sequence":"first","affiliation":[{"name":"Escuela Nacional de Ciencias Biol\u00f3gicas-Campus Zacatenco, Instituto Polit\u00e9cnico Nacional, Unidad Profesional Adolfo L\u00f3pez Mateos, Zacatenco, Av. Wilfrido Massieu 399, Col. Nueva Industrial Vallejo, C.P. Alcald\u00eda Gustavo A. Madero 07738, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3359-4517","authenticated-orcid":false,"given":"Edgar","family":"Torres-Maravilla","sequence":"additional","affiliation":[{"name":"INRAE, AgroParisTech, Micalis Institute, Universit\u00e9 Paris-Saclay, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lino","family":"Mayorga-Reyes","sequence":"additional","affiliation":[{"name":"Departament of Biological Systems, Universidad Aut\u00f3noma Metropolitana Xochimilco, Calzada del Hueso, 1100, Coyoac\u00e1n 04960, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebeca","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"INRAE, AgroParisTech, Micalis Institute, Universit\u00e9 Paris-Saclay, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Langella","sequence":"additional","affiliation":[{"name":"INRAE, AgroParisTech, Micalis Institute, Universit\u00e9 Paris-Saclay, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1069-5558","authenticated-orcid":false,"given":"Ricardo","family":"P\u00e9rez-Past\u00e9n-Borja","sequence":"additional","affiliation":[{"name":"Escuela Nacional de Ciencias Biol\u00f3gicas-Campus Zacatenco, Instituto Polit\u00e9cnico Nacional, Unidad Profesional Adolfo L\u00f3pez Mateos, Zacatenco, Av. Wilfrido Massieu 399, Col. Nueva Industrial Vallejo, C.P. Alcald\u00eda Gustavo A. Madero 07738, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda E.","family":"S\u00e1nchez-Pardo","sequence":"additional","affiliation":[{"name":"Escuela Nacional de Ciencias Biol\u00f3gicas-Campus Zacatenco, Instituto Polit\u00e9cnico Nacional, Unidad Profesional Adolfo L\u00f3pez Mateos, Zacatenco, Av. Wilfrido Massieu 399, Col. Nueva Industrial Vallejo, C.P. Alcald\u00eda Gustavo A. Madero 07738, Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7525-3131","authenticated-orcid":false,"given":"Luis G.","family":"Berm\u00fadez-Humar\u00e1n","sequence":"additional","affiliation":[{"name":"INRAE, AgroParisTech, Micalis Institute, Universit\u00e9 Paris-Saclay, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1038\/nrgastro.2014.66","article-title":"Expert consensus document: The international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic","volume":"11","author":"Hill","year":"2014","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1111\/j.1472-765X.2006.01913.x","article-title":"Effects of Lactobacillus strains on cancer cell proliferation and oxidative stress in vitro","volume":"42","author":"Choi","year":"2006","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.jfda.2018.01.002","article-title":"Benefaction of probiotics for human health: A review","volume":"26","author":"Patra","year":"2018","journal-title":"J. Food Drug Anal."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2493","DOI":"10.3168\/jds.S0022-0302(93)77584-0","article-title":"Effect of antioxidative lactic acid bacteria on rats fed a diet deficient in vitamin E","volume":"76","author":"Kaizu","year":"1993","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Lushchak, V. (2012). Probiotics and oxidative stress. Oxidative Stress Environmental Induction and Dietary Antioxidants, InTech.","DOI":"10.5772\/2536"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/s10620-010-1307-1","article-title":"Antioxidant properties of probiotics and their protective effects in the pathogenesis of radiation-induced enteritis and colitis","volume":"56","author":"Spyropoulos","year":"2011","journal-title":"Dig. Dis. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7887","DOI":"10.1007\/s11033-012-1633-9","article-title":"Antioxidative potential of lactobacilli isolated from the gut of Indian people","volume":"39","author":"Achuthan","year":"2012","journal-title":"Mol. Biol. Rep."},{"key":"ref_8","first-page":"37626","article-title":"Cellular model to assess the antioxidant activity of lactobacilli","volume":"5","author":"Xing","year":"2015","journal-title":"R. Soc. Chem. Adv."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2018.00157","article-title":"Adaptation to aerobic environment of Lactobacillus johnsonii\/gasseri strains","volume":"9","author":"Maresca","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3615","DOI":"10.1021\/jf506326t","article-title":"Probiotics as Potential Antioxidants: A Systematic Review","volume":"63","author":"Mishra","year":"2015","journal-title":"J. Agric. Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1002\/jobm.200800047","article-title":"Isolation and preliminary probiotic selection of lactobacilli from koumiss in Inner Mongolia","volume":"49","author":"Wu","year":"2009","journal-title":"J. Basic Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.ijfoodmicro.2011.05.003","article-title":"Evaluation of probiotic characteristics of newly isolated Lactobacillus spp.: Immune modulation and longevity","volume":"148","author":"Lee","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Torres-Maravilla, E., Lenoir, M., Mayorga-Reyes, L., Allain, T., Sokol, H., Langella, P., S\u00e1nchez-Pardo, M.E., and Berm\u00fadez-Humar\u00e1n, L.G. (2015). Identification of novel anti-inflammatory probiotic strains isolated from pulque. Appl. Microbiol. Biotechnol.","DOI":"10.1007\/s00253-015-7049-4"},{"key":"ref_14","first-page":"32","article-title":"Mechanization process in the production of mezcal","volume":"5","year":"2007","journal-title":"J. Food Agric. Environ."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1111\/j.1472-765X.2008.02359.x","article-title":"Identification of yeast and bacteria involved in the mezcal fermentation of Agave salmiana","volume":"46","author":"Blaschek","year":"2008","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1128\/jb.173.2.697-703.1991","article-title":"16S Ribosomal DNA Amplification for Phylogenetic Study","volume":"173","author":"Weisburg","year":"1991","journal-title":"J. Bacteriol."},{"key":"ref_17","first-page":"1","article-title":"Effects of Sulfasalazine, Lactobacillus Plantarum (Lp-115) and Fish Oil in Experimental Colitis","volume":"1","author":"Paroschi","year":"2015","journal-title":"J. Food Nutr. Disord."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1111\/jam.14229","article-title":"Enhancement of bile resistance by maltodextrin supplementation in Lactobacillus plantarum Lp-115","volume":"126","author":"Zhou","year":"2019","journal-title":"J. Appl. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.ijfoodmicro.2006.01.029","article-title":"Lactobacillus spp. with in vitro probiotic properties from human faeces and traditional fermented products","volume":"109","author":"Franz","year":"2006","journal-title":"Int. J. Food Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1128\/AEM.03075-12","article-title":"Identification of one novel candidate probiotic Lactobacillus plantarum strain active against influenza virus infection in mice by a large-scale screening","volume":"79","author":"Kechaou","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1111\/j.1365-2672.2009.04508.x","article-title":"Characterization of a fibronectin-binding protein from Lactobacillus casei BL23","volume":"108","author":"Monedero","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5189","DOI":"10.1007\/s00253-015-6425-4","article-title":"Antioxidant properties of wine lactic acid bacteria: Oenococcus oeni","volume":"99","author":"Su","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1111\/j.1365-2672.2009.04294.x","article-title":"Free radical scavenging activity of Lactobacillus fermentum in vitro and its antioxidative effect on growing-finishing pigs","volume":"107","author":"Wang","year":"2009","journal-title":"J. Appl. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/j.tiv.2008.02.003","article-title":"Peroxide-induced cell death and lipid peroxidation in C6 glioma cells","volume":"22","author":"Linden","year":"2008","journal-title":"Toxicol. Vitr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.toxrep.2017.12.009","article-title":"Hepatoprotective effect of food preservatives (butylated hydroxyanisole, butylated hydroxytoluene) on carbon tetrachloride-induced hepatotoxicity in rat","volume":"5","author":"Dassarma","year":"2018","journal-title":"Toxicol. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1097\/01.MIB.0000440815.76627.64","article-title":"The commensal bacterium faecalibacterium prausnitzii is protective in DNBS-induced chronic moderate and severe colitis models","volume":"20","author":"Chain","year":"2014","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Mart\u00edn, R., Miquel, S., Chain, F., Natividad, J.M., Jury, J., Lu, J., Sokol, H., Theodorou, V., Bercik, P., and Verdu, E.F. (2015). Faecalibacterium prausnitzii prevents physiological damages in a chronic low-grade inflammation murine model. BMC Microbiol., 15.","DOI":"10.1186\/s12866-015-0400-1"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Bradley, P., Priebat, D., and Christensen, R. (1982). Measurement of cutaneous inflammation: Estimation of neutrophil content with an enzyme marker. J. Investig. Dermatol., 206\u2013209.","DOI":"10.1111\/1523-1747.ep12506462"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1007\/s00284-010-9636-z","article-title":"Culture-Independent Analysis of Lactic Acid Bacteria Diversity Associated with Mezcal Fermentation","volume":"61","year":"2010","journal-title":"Curr. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Douillard, F.P., Ribbera, A., Kant, R., Pietil\u00e4, T.E., J\u00e4rvinen, H.M., Messing, M., Randazzo, C.L., Paulin, L., Laine, P., and Ritari, J. (2013). Comparative Genomic and Functional Analysis of 100 Lactobacillus rhamnosus Strains and Their Comparison with Strain GG. PLoS Genet., 9.","DOI":"10.1371\/journal.pgen.1003683"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.aoas.2019.05.004","article-title":"Isolation and characterization of potential probiotic Lactobacilli from leaves of food plants for possible additives in pellet feeding","volume":"64","author":"Samedi","year":"2019","journal-title":"Ann. Agric. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1263\/jbb.106.381","article-title":"Biofilm Formation by Lactic Acid Bacteria and Resistance to Environmental Stress","volume":"106","author":"Kubota","year":"2008","journal-title":"J. Biosci. Bioeng."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3060","DOI":"10.1128\/AEM.71.6.3060-3067.2005","article-title":"Survival of probiotic lactobacilli in acidic environments is enhanced in the presence of metabolizable sugars","volume":"71","author":"Corcoran","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s12602-018-9430-y","article-title":"Safety Evaluation and Colonisation Abilities of Four Lactic Acid Bacteria as Future Probiotics","volume":"11","author":"Dlamini","year":"2019","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1128\/aem.63.5.1725-1731.1997","article-title":"Cell surface characteristics of Lactobacillus casei subsp. casei, Lactobacillus paracasei subsp. paracasei, and Lactobacillus rhamnosus strains","volume":"63","author":"Pelletier","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Yang, S.J., Kim, K.T., Kim, T.Y., and Paik, H.D. (2020). Probiotic properties and antioxidant activities of Pediococcus pentosaceus SC28 and Levilactobacillus brevis KU15151 in fermented black gamju. Foods, 9.","DOI":"10.3390\/foods9091154"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"8684","DOI":"10.1128\/AEM.02287-12","article-title":"Functional Analysis of the Lactobacillus casei BL23 Sortases","volume":"78","author":"Collado","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1007\/s12602-019-09604-y","article-title":"Probiotic Properties of Lactobacillus Strains Isolated from Table Olive Biofilms","volume":"12","author":"Langella","year":"2020","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1007\/s10059-012-2266-5","article-title":"Lactobacillus plantarum lipoteichoic acid alleviates TNF-\u03b1-induced inflammation in the HT-29 intestinal epithelial cell line","volume":"33","author":"Kim","year":"2012","journal-title":"Mol. Cells"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2018.02639","article-title":"Lactobacillus rhamnosus R0011 and Lactobacillus helveticus R0389 is mediated by secreted bioactive molecules","volume":"9","author":"Jeffrey","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.4049\/jimmunol.1901440","article-title":"Secretome-Mediated Interactions with Intestinal Epithelial Cells: A Role for Secretome Components from Lactobacillus rhamnosus R0011 in the Attenuation of Salmonella enterica Serovar Typhimurium Secretome and TNF-\u03b1\u2013Induced Proinflammatory Responses","volume":"204","author":"Jeffrey","year":"2020","journal-title":"J. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1016\/j.foodchem.2012.06.048","article-title":"Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods","volume":"135","author":"Li","year":"2012","journal-title":"Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1023\/A:1005577330695","article-title":"Antioxidative effect of intestinal bacteria Bifidobacterium longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356","volume":"45","author":"Lin","year":"2000","journal-title":"Dig. Dis. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0168-1605(00)00456-6","article-title":"Determination of peroxy radical-scavenging of lactic acid bacteria","volume":"64","author":"Stecchini","year":"2001","journal-title":"Int. J. Food Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5254","DOI":"10.1128\/jb.177.18.5254-5260.1995","article-title":"Stress response in Lactococcus lactis: Cloning, expression analysis, and mutation of the lactococcal superoxide dismutase gene","volume":"177","author":"Sanders","year":"1995","journal-title":"J. Bacteriol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"90","DOI":"10.5713\/ajas.2005.90","article-title":"Identification and characterization of hydrogen peroxide-generating Lactobacillus fermentum CS12-1","volume":"18","author":"Kang","year":"2005","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11274-018-2410-2","article-title":"A bioluminescent test system reveals valuable antioxidant properties of lactobacillus strains from human microbiota","volume":"34","author":"Marsova","year":"2018","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0168-1605(01)00674-2","article-title":"Two antioxidative lactobacilli strains as promising probiotics","volume":"72","author":"Kullisaar","year":"2002","journal-title":"Int. J. Food Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Morampudi, V., Bhinder, G., Wu, X., Dai, C., Sham, H.P., Vallance, B.A., and Jacobson, K. (2014). DNBS\/TNBS colitis models: Providing insights into inflammatory bowel disease and effects of dietary fat. J. Vis. Exp., 1\u20138.","DOI":"10.3791\/51297"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s00394-013-0506-x","article-title":"Lactobacillus GG-fermented milk prevents DSS-induced colitis and regulates intestinal epithelial homeostasis through activation of epidermal growth factor receptor","volume":"53","author":"Yoda","year":"2014","journal-title":"Eur. J. Nutr."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2019.00477","article-title":"A novel postbiotic from lactobacillus rhamnosus GG with a beneficial effect on intestinal barrier function","volume":"10","author":"Gao","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1053\/gast.1996.v111.pm8690198","article-title":"The effects of Lactobacillus strains and oat fiber on methotrexate- induced enterocolitis in rats","volume":"111","author":"Mao","year":"1996","journal-title":"Gastroenterology"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Hansberry, D.R., Shah, K., Agarwal, P., and Agarwal, N. (2017). Fecal Myeloperoxidase as a Biomarker for Inflammatory Bowel Disease. Cureus.","DOI":"10.7759\/cureus.1004"},{"key":"ref_54","first-page":"35","article-title":"Defining microbiota for developing new probiotics","volume":"23","author":"Collado","year":"2012","journal-title":"Microb. Ecol. Health Dis."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/5\/1063\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:00:54Z","timestamp":1760162454000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/5\/1063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,14]]},"references-count":54,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["microorganisms9051063"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms9051063","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,14]]}}}