{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T12:14:40Z","timestamp":1789647280454,"version":"4.0.0"},"reference-count":32,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,14]],"date-time":"2021-05-14T00:00:00Z","timestamp":1620950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002745","name":"Bayerische Forschungsstiftung","doi-asserted-by":"publisher","award":["AZ-1300-17"],"award-info":[{"award-number":["AZ-1300-17"]}],"id":[{"id":"10.13039\/501100002745","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["KA3492\/6-1"],"award-info":[{"award-number":["KA3492\/6-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Schu2446\/4-1"],"award-info":[{"award-number":["Schu2446\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Viral diseases remain a major concern for animal health and global food production in modern agriculture. In chickens, avian leukosis virus subgroup J (ALV-J) represents an important pathogen that causes severe economic loss. Until now, no vaccine or antiviral drugs are available against ALV-J and strategies to combat this pathogen in commercial flocks are desperately needed. CRISPR\/Cas9 targeted genome editing recently facilitated the generation of genetically modified chickens with a mutation of the chicken ALV-J receptor Na+\/H+ exchanger type 1 (chNHE1). In this study, we provide evidence that this mutation protects a commercial chicken line (NHE1\u0394W38) against the virulent ALV-J prototype strain HPRS-103. We demonstrate that replication of HPRS-103 is severely impaired in NHE1\u0394W38 birds and that ALV-J-specific antigen is not detected in cloacal swabs at later time points. Consistently, infected NHE1\u0394W38 chickens gained more weight compared to their non-transgenic counterparts (NHE1W38). Histopathology revealed that NHE1W38 chickens developed ALV-J typical pathology in various organs, while no pathological lesions were detected in NHE1\u0394W38 chickens. Taken together, our data revealed that this mutation can render a commercial chicken line resistant to highly pathogenic ALV-J infection, which could aid in fighting this pathogen and improve animal health in the field.<\/jats:p>","DOI":"10.3390\/microorganisms9051066","type":"journal-article","created":{"date-parts":[[2021,5,16]],"date-time":"2021-05-16T23:17:16Z","timestamp":1621207036000},"page":"1066","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["A Genetically Engineered Commercial Chicken Line Is Resistant to Highly Pathogenic Avian Leukosis Virus Subgroup J"],"prefix":"10.3390","volume":"9","author":[{"given":"Ahmed","family":"Kheimar","sequence":"first","affiliation":[{"name":"Institute of Virology, Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"},{"name":"Department of Poultry Diseases, Faculty of Veterinary Medicine, Sohag University, 82424 Sohag, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romina","family":"Klinger","sequence":"additional","affiliation":[{"name":"Reproductive Biotechnology, Department of Molecular Life Sciences, TUM School of Life Sciences, Technical University Munich, 85354 Freising, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0698-5395","authenticated-orcid":false,"given":"Luca D.","family":"Bertzbach","sequence":"additional","affiliation":[{"name":"Institute of Virology, Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5608-8166","authenticated-orcid":false,"given":"Hicham","family":"Sid","sequence":"additional","affiliation":[{"name":"Reproductive Biotechnology, Department of Molecular Life Sciences, TUM School of Life Sciences, Technical University Munich, 85354 Freising, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Virology, Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9975-0642","authenticated-orcid":false,"given":"Andel\u00e9 M.","family":"Conradie","sequence":"additional","affiliation":[{"name":"Institute of Virology, Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Schade","sequence":"additional","affiliation":[{"name":"Bavarian Animal Health Service, Department of Pathology, 85586 Poing, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"B\u00f6hm","sequence":"additional","affiliation":[{"name":"Bavarian Animal Health Service, Department of Pathology, 85586 Poing, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Preisinger","sequence":"additional","affiliation":[{"name":"EW Group GmbH, 49429 Visbek, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8255-516X","authenticated-orcid":false,"given":"Venugopal","family":"Nair","sequence":"additional","affiliation":[{"name":"The Pirbright Institute, Woking GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1328-2695","authenticated-orcid":false,"given":"Benedikt B.","family":"Kaufer","sequence":"additional","affiliation":[{"name":"Institute of Virology, Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9990-1890","authenticated-orcid":false,"given":"Benjamin","family":"Schusser","sequence":"additional","affiliation":[{"name":"Reproductive Biotechnology, Department of Molecular Life Sciences, TUM School of Life Sciences, Technical University Munich, 85354 Freising, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1080\/03079450601102921","article-title":"Differences in pathogenicity among strains of the same or different avian leukosis virus subgroups","volume":"36","author":"Stepanets","year":"2007","journal-title":"Avian Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1111\/tbed.13023","article-title":"The emergence of the infection of subgroup J avian leucosis virus escalated the tumour incidence in commercial Yellow chickens in Southern China in recent years","volume":"66","author":"Li","year":"2019","journal-title":"Transbound. 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