{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:01:29Z","timestamp":1760234489884,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T00:00:00Z","timestamp":1621468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["773830"],"award-info":[{"award-number":["773830"]}]},{"name":"German Federal Institute for Risk Assessment","award":["43-001 and 43-002"],"award-info":[{"award-number":["43-001 and 43-002"]}]},{"name":"German Federal Ministry of Education and Research (BMBF)","award":["01KI2009D"],"award-info":[{"award-number":["01KI2009D"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Due to the increasing reports of carbapenemase-producing Enterobacteriaceae (CPE) from livestock in recent years, the European Reference Laboratory for Antimicrobial Resistances (EURL-AR) provided a protocol for their recovery from caecum and meat samples. This procedure exhibited limitations for the detection of CPE with low carbapenem MIC values. Therefore, it was modified by a second, selective enrichment in lysogeny broth with cefotaxime (CTX 1 mg\/L) and with meropenem (MEM 0.125 mg\/L) at 37 \u00b0C under microaerophilic conditions. By Real-time PCR, these enrichments are pre-screened for the most common carbapenemase genes. Another adaptation was the use of in-house prepared MacConkey agar with MEM and MEM+CTX instead of commercial selective agar. According to the EURL-method, we achieved 100% sensitivity and specificity using the in-house media instead of commercial agar, which decreased the sensitivity to ~75%. Comparing the method with and without the second enrichment, no substantial influence on sensitivity and specificity was detected. Nevertheless, this enrichment has simplified the CPE-isolation regarding the accompanying microbiota and the separation of putative colonies. In conclusion, the sensitivity of the method can be increased with slight modifications.<\/jats:p>","DOI":"10.3390\/microorganisms9051105","type":"journal-article","created":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T11:45:57Z","timestamp":1621511157000},"page":"1105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation Procedure for CP E. coli from Caeca Samples under Review towards an Increased Sensitivity"],"prefix":"10.3390","volume":"9","author":[{"given":"Natalie","family":"Pauly","sequence":"first","affiliation":[{"name":"German Federal Institute for Risk Assessment (Bundesinstitut f\u00fcr Risikobewertung), D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Klaar","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (Bundesinstitut f\u00fcr Risikobewertung), D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"Skladnikiewicz-Ziemer","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (Bundesinstitut f\u00fcr Risikobewertung), D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"Juraschek","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (Bundesinstitut f\u00fcr Risikobewertung), D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8619-1498","authenticated-orcid":false,"given":"Mirjam","family":"Grobbel","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (Bundesinstitut f\u00fcr Risikobewertung), D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6930-4358","authenticated-orcid":false,"given":"Jens Andr\u00e9","family":"Hammerl","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (Bundesinstitut f\u00fcr Risikobewertung), D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukas","family":"Hemmers","sequence":"additional","affiliation":[{"name":"State Office for Health and Social Affairs Berlin, D-10639 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2966-2713","authenticated-orcid":false,"given":"Annemarie","family":"K\u00e4sbohrer","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (Bundesinstitut f\u00fcr Risikobewertung), D-10589 Berlin, Germany"},{"name":"Unit for Veterinary Public Health and Epidemiology, University of Veterinary Medicine, AT-1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6303-8212","authenticated-orcid":false,"given":"Stefan","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Epizootics, Freie Universit\u00e4t Berlin, D-14163 Berlin, Germany"},{"name":"FAO Reference Centre for Antimicrobial Resistance, Freie Universit\u00e4t Berlin, D-14163 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4824-048X","authenticated-orcid":false,"given":"Diana","family":"Meemken","sequence":"additional","affiliation":[{"name":"Institute of Food Safety and Food Hygiene, Freie Universit\u00e4t Berlin, D-14163 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd-Alois","family":"Tenhagen","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (Bundesinstitut f\u00fcr Risikobewertung), D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1266-9978","authenticated-orcid":false,"given":"Alexandra","family":"Irrgang","sequence":"additional","affiliation":[{"name":"German Federal Institute for Risk Assessment (Bundesinstitut f\u00fcr Risikobewertung), D-10589 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,20]]},"reference":[{"key":"ref_1","unstructured":"WHO (2019). 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