{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:19:29Z","timestamp":1790835569309,"version":"4.1.0"},"reference-count":102,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T00:00:00Z","timestamp":1621555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>CC17 Streptococcus agalactiae carrying group-A prophages is increasingly responsible for neonatal infections. To investigate the impact of the genetic features of a group-A prophage, we first conducted an in silico analysis of the genome of 12\/111phiA, a group-A prophage carried by a strain responsible for a bloodstream infection in a parturient. This revealed a Restriction Modification system, suggesting a prophage maintenance strategy and five ORFs of interest for the host and encoding a type II toxin antitoxin system RelB\/YafQ, an endonuclease, an S-adenosylmethionine synthetase MetK, and an StrP-like adhesin. Using the WT strain cured from 12\/111phiA and constructing deleted mutants for the ORFs of interest, and their complemented mutants, we demonstrated an impact of prophage features on growth characteristics, cell morphology and biofilm formation. Our findings argue in favor of 12\/111phiA domestication by the host and a role of prophage features in cell autoaggregation, glycocalyx and biofilm formation. We suggest that lysogeny may promote GBS adaptation to the acid environment of the vagina, consequently colonizing and infecting neonates.<\/jats:p>","DOI":"10.3390\/microorganisms9061112","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T00:01:20Z","timestamp":1621814480000},"page":"1112","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["12\/111phiA Prophage Domestication Is Associated with Autoaggregation and Increased Ability to Produce Biofilm in Streptococcus agalactiae"],"prefix":"10.3390","volume":"9","author":[{"given":"Ad\u00e9la\u00efde","family":"Renard","sequence":"first","affiliation":[{"name":"UMR INRAE 1282 Infectiologie et Sant\u00e9 Publique, Bact\u00e9ries et Risque Materno-Foetal, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6466-2324","authenticated-orcid":false,"given":"Seydina M.","family":"Diene","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, MEPHI, IRD, APHM, IHU-M\u00e9diterran\u00e9e Infection, Facult\u00e9 de Pharmacie, 13000 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luka","family":"Courtier-Martinez","sequence":"additional","affiliation":[{"name":"UMR INRAE 1282 Infectiologie et Sant\u00e9 Publique, Bact\u00e9ries et Risque Materno-Foetal, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5171-9873","authenticated-orcid":false,"given":"Julien Burlaud","family":"Gaillard","sequence":"additional","affiliation":[{"name":"Plateforme IBiSA Microscopie Electronique, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1825-9358","authenticated-orcid":false,"given":"Houssein","family":"Gbaguidi-Haore","sequence":"additional","affiliation":[{"name":"Service d\u2019Hygi\u00e8ne Hospitali\u00e8re, CHRU, 25056 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Mereghetti","sequence":"additional","affiliation":[{"name":"UMR INRAE 1282 Infectiologie et Sant\u00e9 Publique, Bact\u00e9ries et Risque Materno-Foetal, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Quentin","sequence":"additional","affiliation":[{"name":"UMR INRAE 1282 Infectiologie et Sant\u00e9 Publique, Bact\u00e9ries et Risque Materno-Foetal, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0540-750X","authenticated-orcid":false,"given":"Patrice","family":"Francois","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche G\u00e9nomique, Service des Maladies Infectieuses, Centre M\u00e9dical Universitaire, H\u00f4pitaux Universitaire de Gen\u00e8ve, 1205 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Van Der Mee-Marquet","sequence":"additional","affiliation":[{"name":"UMR INRAE 1282 Infectiologie et Sant\u00e9 Publique, Bact\u00e9ries et Risque Materno-Foetal, Universit\u00e9 de Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/s10096-017-3125-2","article-title":"Intrapartum group B Streptococcus screening in the labor ward by Xpert(R) GBS real-time PCR","volume":"37","author":"Plainvert","year":"2018","journal-title":"Eur. 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