{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:11:21Z","timestamp":1790107881698,"version":"4.0.1"},"reference-count":68,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Minist\u00e8re de l'Agriculture et de l'Alimentation (Ecoantibio plan) and Oniris","award":["OPE-2018-0025"],"award-info":[{"award-number":["OPE-2018-0025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>This study presents the occurrence and abundance of Aeromonas antibiotic-resistant bacteria (ARB) and genes (ARGs) isolated from water, biofilm and fish in two commercial trout farms before and one week after flumequine treatment. Wild (WT) and non-wild (NWT) strains were determined for quinolones (flumequine, oxolinic acid and enrofloxacin), oxytetracycline (OXY), florfenicol (FFN), trimethoprim-sulfamethoxazole (TMP) and colistin (COL), and pMAR (presumptive multi-resistant) strains were classified. Forty-four ARGs for the mentioned antibiotics, \u03b2-lactams and multi-resistance were quantified for 211 isolates. BlaSHV-01, mexF and tetE were the dominant ARGs. A greater occurrence and abundance of tetA2, sul3, floR1, blaSHV-01 and mexF were observed for NWT compared to WT. The occurrence of pMAR and NWT Aeromonas for quinolones, OXY, FFN, TMP, COL and ARGs depended on the Aeromonas origin, antibiotic use and the presence of upstream activities. Our results revealed the impact of a flumequine treatment on Aeromonas present on a fish farm through an increase in NWT and pMAR strains. The link between fish and their environment was shown by the detection of identical ARB and ARGs in the two types of samples. There appears to be a high risk of resistance genes developing and spreading in aquatic environments.<\/jats:p>","DOI":"10.3390\/microorganisms9061201","type":"journal-article","created":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T15:58:44Z","timestamp":1622563124000},"page":"1201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Antimicrobial Susceptibility Profiles and Resistance Genes in Genus Aeromonas spp. Isolated from the Environment and Rainbow Trout of Two Fish Farms in France"],"prefix":"10.3390","volume":"9","author":[{"given":"Niki","family":"Hayatgheib","sequence":"first","affiliation":[{"name":"INRAE, Oniris, BIOEPAR, 44300 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9gol\u00e8ne","family":"Calvez","sequence":"additional","affiliation":[{"name":"INRAE, Oniris, BIOEPAR, 44300 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Fournel","sequence":"additional","affiliation":[{"name":"INRAE, Oniris, BIOEPAR, 44300 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Pineau","sequence":"additional","affiliation":[{"name":"INRAE, Oniris, BIOEPAR, 44300 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6494-4700","authenticated-orcid":false,"given":"Herv\u00e9","family":"Pouliquen","sequence":"additional","affiliation":[{"name":"INRAE, Oniris, BIOEPAR, 44300 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0051-4592","authenticated-orcid":false,"given":"Emmanuelle","family":"Moreau","sequence":"additional","affiliation":[{"name":"INRAE, Oniris, BIOEPAR, 44300 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.tim.2013.11.001","article-title":"The role of aquatic ecosystems as reservoirs of antibiotic resistance","volume":"22","author":"Marti","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1038\/nrmicro3439","article-title":"Tackling antibiotic resistance: The environmental framework","volume":"13","author":"Berendonk","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/nyas.14036","article-title":"Complexities in understanding antimicrobial resistance across domesticated animal, human, and environmental systems","volume":"1441","author":"Graham","year":"2019","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1111\/1462-2920.12134","article-title":"Antimicrobial use in aquaculture re-examined: Its relevance to antimicrobial resistance and to animal and human health","volume":"15","author":"Cabello","year":"2013","journal-title":"Environ. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Watts, J.E.M., Schreier, H.J., Lanska, L., and Hale, M.S. (2017). The rising tide of antimicrobial resistance in aquaculture: Sources, sinks and solutions. Mar. Drugs, 15.","DOI":"10.3390\/md15060158"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ijantimicag.2018.03.010","article-title":"Antimicrobial resistance in aquaculture: Current knowledge and alternatives to tackle the problem","volume":"52","author":"Santos","year":"2018","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.ijfoodmicro.2006.09.030","article-title":"Characterization of integrons and tetracycline resistance determinants in Aeromonas spp. isolated from South African aquaculture systems","volume":"114","author":"Jacobs","year":"2007","journal-title":"Int. J. Food Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.ijantimicag.2007.10.002","article-title":"Antibiotic resistance of motile aeromonads in indoor catfish and eel farms in the southern part of The Netherlands","volume":"31","author":"Penders","year":"2008","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.aquaculture.2011.03.006","article-title":"Antimicrobial resistance of Aeromonas spp. isolated from the growth pond to the commercial product in a rainbow trout farm following a flumequine treatment","volume":"315","author":"Naviner","year":"2011","journal-title":"Aquaculture"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1007\/s13213-014-0911-2","article-title":"The prevalence of antibiotic resistance genes among Aeromonas species in aquatic environments","volume":"64","author":"Piotrowska","year":"2014","journal-title":"Ann. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.3389\/fmicb.2016.01875","article-title":"Evidence of increased antibiotic resistance in phylogenetically-diverse aeromonas isolates from semi-intensive fish ponds treated with antibiotics","volume":"7","author":"Patil","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1111\/raq.12344","article-title":"Antibiotic use in aquaculture, policies and regulation, health and environmental risks: A review of the top 15 major producers","volume":"12","author":"Lulijwa","year":"2020","journal-title":"Rev. Aquac."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11908","DOI":"10.1021\/acs.jafc.0c03996","article-title":"Antibiotics and food safety in aquaculture","volume":"68","author":"Sun","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Romero, J., Feijo\u00f3, C.G., and Navarrete, P. (2012). Antibiotics in Aquaculture\u2014Use, Abuse and Alternatives, InTech Open.","DOI":"10.5772\/28157"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"55617","DOI":"10.19080\/NFSIJ.2017.03.555617","article-title":"Prospects of Medicated Feed in Aquaculture","volume":"3","author":"Ranjan","year":"2017","journal-title":"Nutr. Food Sci. Int. J."},{"key":"ref_16","first-page":"269","article-title":"Abuse of Antibiotics in Aquaculture and its Effects on Human, Aquatic Animal and Environment","volume":"2","author":"Rasu","year":"2017","journal-title":"Haya Saudi J. Life Sci."},{"key":"ref_17","first-page":"37","article-title":"The social life of Aeromonas through biofilm and quorum sensing systems","volume":"8","author":"Lamy","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"fiw052","DOI":"10.1093\/femsec\/fiw052","article-title":"Aquaculture changes the profile of antibiotic resistance and mobile genetic element associated genes in Baltic Sea sediments","volume":"92","author":"Muziasari","year":"2016","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.3389\/fmicb.2016.02137","article-title":"The resistome of farmed fish feces contributes to the enrichment of antibiotic resistance genes in sediments below baltic sea fish farms","volume":"7","author":"Muziasari","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_20","first-page":"1","article-title":"Aeromonas et a\u00e9romonoses en m\u00e9decine humaine","volume":"7","author":"Lamy","year":"2012","journal-title":"Colloids Surfaces A Physicochem. Eng. Asp."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez-Bravo, A., and Figueras, M.J. (2020). An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity. Microorganisms, 8.","DOI":"10.3390\/microorganisms8010129"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1128\/IAI.72.4.1939-1945.2004","article-title":"Aeromonas Flagella (Polar and Lateral) Are Enterocyte Adhesins That Contribute to Biofilm Formation on Surfaces","volume":"72","author":"Kirov","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/aah.10056","article-title":"Biofilm and Sediment are Major Reservoirs of Virulent Aeromonas hydrophila (vAh) in Catfish Production Ponds","volume":"31","author":"Cai","year":"2019","journal-title":"J. Aquat. Anim. Health"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.prevetmed.2014.11.001","article-title":"Farm size, seining practices, and salt use: Risk factors for Aeromonas hydrophila outbreaks in farm-raised catfish, Alabama, USA","volume":"118","author":"Bebak","year":"2015","journal-title":"Prev. Vet. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e02220","DOI":"10.1016\/j.heliyon.2019.e02220","article-title":"Phenotypic and molecular detection of Aeromonads infection in farmed Nile tilapia in Southern highland and Northern Tanzania","volume":"5","author":"Mzula","year":"2019","journal-title":"Heliyon"},{"key":"ref_26","unstructured":"ANSES (2015). Hi\u00e9rarchisation des Dangers Sanitaires Exotiques ou Pr\u00e9sents en France M\u00e9tropolitaine Chez les Poissons D\u2019\u00e9levage, ANSES. Avis l\u2019Anses Saisine n\u00b0 2013-SA-0049 C."},{"key":"ref_27","unstructured":"FranceAgriMer (2019). Les Fili\u00e8res P\u00eache et Aquaculture en France, FranceAgriMer."},{"key":"ref_28","first-page":"207","article-title":"Resistance to \u03b2-lactam antibiotics in Aeromonas hydrophila isolated from rainbow trout (Oncorhynchus mykiss)","volume":"7","author":"Saavedra","year":"2004","journal-title":"Int. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.vetmic.2014.06.012","article-title":"Re-identification of Aeromonas isolates from rainbow trout and incidence of class 1 integron and \u03b2-lactamase genes","volume":"172","author":"Figueras","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/lam.13363","article-title":"The determination of antimicrobial susceptibility by MIC and epidemiological cut-off values and the detection of resistance genes in Aeromonas species isolated from cultured fish","volume":"71","author":"Duman","year":"2020","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"503","DOI":"10.3389\/fmicb.2017.00503","article-title":"Aeromonas diversity and antimicrobial susceptibility in freshwater-An attempt to set generic epidemiological cut-off values","volume":"8","author":"Baron","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_32","unstructured":"EUCAST (European Committee on Antimicrobial Susceptibility) (2021, January 18). Testing Breakpoint Tables for Interpretation of MICs and Zone Diameters. Available online: http:\/\/www.eucast.org\/fileadmin\/src\/media\/PDFs\/EUCAST_files\/Breakpoint_tables\/v_5.0_Breakpoint_Table_01.pdf."},{"key":"ref_33","unstructured":"Cannon, R.M., and Roe, R.T. (1982). Livestock Disease Surveys: A Field Manual for Veterinarians, Australian Government Publishing Service."},{"key":"ref_34","first-page":"20","article-title":"Some general epidemiological concepts","volume":"2007","author":"Thrusfield","year":"2007","journal-title":"Vet. Epidemiol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Corry, J.E.L., Curtis, G.D.W., and Baird, D.W. (2011). Handbook of Culture Media for Food and Water Microbiology, Royal Society of Chemistry.","DOI":"10.1039\/9781847551450"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1128\/aem.41.5.1152-1158.1981","article-title":"Membrane filter method for enumerating Escherichia coli","volume":"41","author":"Dufour","year":"1981","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_37","unstructured":"Baribeau, H., Pozos, N.L., Boulos, L., and Crozes, G.F. (2005). Impact of Distribution System Water Quality on Disinfection Efficacy, American Water Works Research Foundation."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"312","DOI":"10.2166\/wh.2009.041","article-title":"DNA-based real-time detection and quantification of aeromonads from fresh water beaches on Lake Ontario","volume":"7","author":"Khan","year":"2009","journal-title":"J. Water Health"},{"key":"ref_39","unstructured":"CLSI (2006). Method for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals, CLSI. CLSI Document VET03-A."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.1007\/s10096-012-1578-x","article-title":"Which antibiotics and breakpoints should be used for Aeromonas susceptibility testing? Considerations from a comparison of agar dilution and disk diffusion methods using Enterobacteriaceae breakpoints","volume":"31","author":"Lamy","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4445","DOI":"10.1128\/JCM.01101-10","article-title":"Normalized resistance interpretation as a tool for establishing epidemiological MIC susceptibility breakpoints","volume":"48","author":"Kronvall","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1111\/j.1469-0691.2006.01377.x","article-title":"Statistical characterisation of bacterial wild-type MIC value distributions and the determination of epidemiological cut-off values","volume":"12","author":"Turnidge","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_44","unstructured":"Promega Corporation (2019). Technical Manual Wizard\u00ae Genomic DNA Purification Kit Wizard\u00ae Genomic DNA Purification Kit. Tech. Bull., 1, 1\u201319."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1473-3099(15)00424-7","article-title":"Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: A microbiological and molecular biological study","volume":"16","author":"Liu","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6","DOI":"10.2807\/1560-7917.ES.2016.21.27.30280","article-title":"Identification of a novel plasmid-mediated colistinresistance gene, mcr-2, in Escherichia coli, Belgium, June 2016","volume":"21","author":"Xavier","year":"2016","journal-title":"Eurosurveillance"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1093\/jac\/dkx327","article-title":"Identification of a novel transposon-associated phosphoethanolamine transferase gene, mcr-5, conferring colistin resistance in d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B","volume":"72","author":"Borowiak","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3070","DOI":"10.3389\/fmicb.2020.590902","article-title":"Antibiotic Resistance Genes and Bacterial Communities of Farmed Rainbow Trout Fillets (Oncorhynchus mykiss)","volume":"11","author":"Helsens","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"fiw014","DOI":"10.1093\/femsec\/fiw014","article-title":"High-throughput quantification of antibiotic resistance genes from an urban wastewater treatment plant","volume":"92","author":"Karkman","year":"2016","journal-title":"FEMS Microbiol. Ecol"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1073\/pnas.1222743110","article-title":"Diverse and abundant antibiotic resistance genes in Chinese swine farms","volume":"110","author":"Zhu","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_51","unstructured":"RStudio Team (2019). RStudio: Integrated Development for R, RStudio, Inc."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1016\/S0723-2020(99)80019-8","article-title":"In vitro susceptibilities of Aeromonas genomic species to 69 antimicrobial agents","volume":"22","author":"Christmann","year":"1999","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1093\/jac\/46.2.297","article-title":"Antimicrobial resistance of mesophilic Aeromonas spp. isolated from two European rivers","volume":"46","author":"Pineau","year":"2000","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_54","unstructured":"CLSI (2020). Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing, CLSI. [30th ed.]. CLSI Suppl. M100."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.aquaculture.2007.07.216","article-title":"Impact of inter-lab variation on the estimation of epidemiological cut-off values for disc diffusion susceptibility test data for Aeromonas salmonicida","volume":"272","author":"Smith","year":"2007","journal-title":"Aquaculture"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1051\/vetres:2001122","article-title":"Importance of integrons in the diffusion of resistance","volume":"32","author":"Arattoli","year":"2001","journal-title":"Vet. Res."},{"key":"ref_57","first-page":"1167","article-title":"Antimicrobial resistance survey in a river receiving effluents from freshwater fish farms","volume":"102","author":"Gordon","year":"2007","journal-title":"J. Appl. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0044-8486(00)00340-9","article-title":"Increase in the prevalence of oxolinic acid resistant Acinetobacter spp. Observed in a stream receiving the effluent from a freshwater trout farm following the treatment with oxolinic acid-medicated feed","volume":"188","author":"Guardabassi","year":"2000","journal-title":"Aquaculture"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.aquaculture.2007.01.012","article-title":"The tetracycline resistance gene tet (E) is frequently occurring and present on large horizontally transferable plasmids in Aeromonas spp. from fish farms","volume":"266","author":"Sichlau","year":"2007","journal-title":"Aquaculture"},{"key":"ref_60","first-page":"135","article-title":"Molecular mechanisms of quinolone resistance in clinical isolates of Aeromonas caviae and Aeromonas veronii bv. sobria","volume":"13","author":"Arias","year":"2010","journal-title":"Int. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ijfoodmicro.2016.04.030","article-title":"Prevalence and characterization of plasmid-mediated quinolone resistance genes in Aeromonas spp. isolated from South African freshwater fish","volume":"231","author":"Chenia","year":"2016","journal-title":"Int. J. Food Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"5561","DOI":"10.1111\/are.13378","article-title":"Determination and transferability of plasmid-mediated antibiotic resistance genes of the bacteria isolated from rainbow trout","volume":"48","author":"Capkin","year":"2017","journal-title":"Aquac. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.14202\/vetworld.2019.1735-1746","article-title":"An overview of colistin resistance, mobilized colistin resistance genes dissemination, global responses, and the alternatives to colistin: A review","volume":"12","author":"Gharaibeh","year":"2019","journal-title":"Vet. World"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"348","DOI":"10.3389\/fcimb.2020.00348","article-title":"Identification of Antimicrobial Resistance Determinants in Aeromonas veronii Strain MS-17-88 Recovered From Channel Catfish (Ictalurus punctatus)","volume":"10","author":"Tekedar","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1128\/AAC.32.9.1314","article-title":"\u03b2-Lactam resistance in Aeromonas spp. caused by inducible \u03b2-lactamases active against penicillins, cephalosporins, and carbapenems","volume":"32","author":"Bakken","year":"1988","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1128\/AAC.01052-07","article-title":"Role of the AheABC efflux pump in Aeromonas hydrophila intrinsic multidrug resistance","volume":"52","author":"Hernould","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"102245","DOI":"10.1016\/j.jece.2018.03.004","article-title":"Prevalence of antibiotics and antibiotic resistance genes in a wastewater effluent-receiving river in the Netherlands","volume":"8","author":"Sabri","year":"2020","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1128\/AAC.01252-10","article-title":"A two-component regulatory system interconnects resistance to polymyxins, aminoglycosides, fluoroquinolones, and \u03b2-lactams in Pseudomonas aeruginosa","volume":"55","author":"Muller","year":"2011","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/6\/1201\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:09:58Z","timestamp":1760162998000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/6\/1201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,1]]},"references-count":68,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["microorganisms9061201"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms9061201","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,1]]}}}