{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T05:04:04Z","timestamp":1778303044797,"version":"3.51.4"},"reference-count":41,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,6]],"date-time":"2021-06-06T00:00:00Z","timestamp":1622937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2019-104812GB-I00"],"award-info":[{"award-number":["PID2019-104812GB-I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["MDM-2017-0737"],"award-info":[{"award-number":["MDM-2017-0737"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The Dallol geothermal area originated as a result of seismic activity and the presence of a shallow underground volcano, both due to the divergence of two tectonic plates. In its ascent, hot water dissolves and drags away the subsurface salts. The temperature of the water that comes out of the chimneys is higher than 100 \u00b0C, with a pH close to zero and high mineral concentration. These factors make Dallol a polyextreme environment. So far, nanohaloarchaeas, present in the salts that form the walls of the chimneys, have been the only living beings reported in this extreme environment. Through the use of complementary techniques: culture in microcosms, methane stable isotope signature and hybridization with specific probes, the methanogenic activity in the Dallol area has been assessed. Methane production in microcosms, positive hybridization with the Methanosarcinales probe and the \u03b413CCH4-values measured, show the existence of extensive methanogenic activity in the hydrogeothermic Dallol system. A methylotrophic pathway, carried out by Methanohalobium and Methanosarcina-like genera, could be the dominant pathway for methane production in this environment.<\/jats:p>","DOI":"10.3390\/microorganisms9061231","type":"journal-article","created":{"date-parts":[[2021,6,6]],"date-time":"2021-06-06T23:59:55Z","timestamp":1623023995000},"page":"1231","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Methanogenesis at High Temperature, High Ionic Strength and Low pH in the Volcanic Area of Dallol, Ethiopia"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3226-3967","authenticated-orcid":false,"given":"Jose L.","family":"Sanz","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular, Universidad Aut\u00f3noma de Madrid, Cantoblanco, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuria","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Centro de Astrobiolog\u00eda (INTA-CSIC) Crtera, Ajalvir km 4 Torrej\u00f3n de Ardoz, 28850 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1240-4144","authenticated-orcid":false,"given":"Cristina","family":"Escudero","sequence":"additional","affiliation":[{"name":"Centro de Astrobiolog\u00eda (INTA-CSIC) Crtera, Ajalvir km 4 Torrej\u00f3n de Ardoz, 28850 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1568-4591","authenticated-orcid":false,"given":"Daniel","family":"Carrizo","sequence":"additional","affiliation":[{"name":"Centro de Astrobiolog\u00eda (INTA-CSIC) Crtera, Ajalvir km 4 Torrej\u00f3n de Ardoz, 28850 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7560-1033","authenticated-orcid":false,"given":"Ricardo","family":"Amils","sequence":"additional","affiliation":[{"name":"Centro de Astrobiolog\u00eda (INTA-CSIC) Crtera, Ajalvir km 4 Torrej\u00f3n de Ardoz, 28850 Madrid, Spain"},{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM), Cantoblanco, 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9977-7060","authenticated-orcid":false,"given":"Felipe","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Centro de Astrobiolog\u00eda (INTA-CSIC) Crtera, Ajalvir km 4 Torrej\u00f3n de Ardoz, 28850 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1126\/science.172.3982.468","article-title":"Final desiccation of the Afar Rifth, Ethiopia","volume":"172","author":"Bonatti","year":"1971","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1130\/GES00070.1","article-title":"Vent distribution and crustal thickness in stretched continental crust: The case of the Afar Depression (Ethiopia)","volume":"3","author":"Mazzarini","year":"2007","journal-title":"Geosphere"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1038\/27417","article-title":"Cenozoic magmatism throughout east Africa resulting from impact of a single plume","volume":"395","author":"Ebinger","year":"1998","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lahitte, P., Gillot, P.Y., Kidane, T., Courtillot, V., and Bekele, A. 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