{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:33:01Z","timestamp":1761989581537,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Cystic echinococcosis (CE) is a zoonosis that is prevalent worldwide. It is considered endemic in Portugal but few studies have been performed on Echinococcus granulosus sensu lato and their hosts. In this study, CE cysts are reported for the first time in a free-living wild boar (Sus scrofa) in Portugal. The presence of the metacestodes in the liver of the wild boar was identified by morphological features, microscopic examination and molecular analysis. The sequencing of part of the DNA nuclear ribosomal internal transcribed spacer-1 (ITS-1) region revealed a G5 genotype that presently corresponds to Echinococcus ortleppi. This is the first report of E. ortleppi in Portugal and to the best of the authors\u2019 knowledge, in Europe. These results suggest that wild boar may be a host of CE, namely, crossing the livestock\u2013wildlife interface, which has important public health implications. Wildlife reservoirs must be taken into account as CE hosts and surveillance of game as well as health education for hunters should be implemented using a One Health approach, with implementation of feasible and tailor-made control strategies, namely, proper elimination of byproducts in the field.<\/jats:p>","DOI":"10.3390\/microorganisms9061256","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T14:16:04Z","timestamp":1623248164000},"page":"1256","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["First Report of Echinococcus ortleppi in Free-Living Wild Boar (Sus scrofa) from Portugal"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1236-3012","authenticated-orcid":false,"given":"Teresa Letra","family":"Mateus","sequence":"first","affiliation":[{"name":"CISAS\u2014Centre for Research and Development in Agrifood Systems and Sustainability, Escola Superior Agr\u00e1ria, Instituto Polit\u00e9cnico de Viana do Castelo, 4900-347 Viana do Castelo, Portugal"},{"name":"Epidemiology Research Unit (EPIUnit), Institute of Public Health, University of Porto, Laboratory for Integrative and Translational Research in Population Health (ITR), 4050-091 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Jo\u00e3o","family":"Gargat\u00e9","sequence":"additional","affiliation":[{"name":"National Reference Laboratory of Parasitic and Fungal Infections, Infectious Diseases Department of the National Institute of Health Dr. Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisboa, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0844-4442","authenticated-orcid":false,"given":"Anabela","family":"Vilares","sequence":"additional","affiliation":[{"name":"National Reference Laboratory of Parasitic and Fungal Infections, Infectious Diseases Department of the National Institute of Health Dr. Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisboa, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Idalina","family":"Ferreira","sequence":"additional","affiliation":[{"name":"National Reference Laboratory of Parasitic and Fungal Infections, Infectious Diseases Department of the National Institute of Health Dr. Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisboa, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Veterin\u00e1rias, Universidade de Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catarina","family":"Coelho","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancia Animal e Veterin\u00e1ria, (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8588-7071","authenticated-orcid":false,"given":"Madalena","family":"Vieira-Pinto","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Veterin\u00e1rias, Universidade de Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Centro de Ci\u00eancia Animal e Veterin\u00e1ria, (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/bs.apar.2016.11.001","article-title":"Global Distribution of Alveolar and Cystic Echinococcosis","volume":"95","author":"Deplazes","year":"2017","journal-title":"Adv. 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