{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T12:42:36Z","timestamp":1791808956641,"version":"4.3.4"},"reference-count":166,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,3]],"date-time":"2021-08-03T00:00:00Z","timestamp":1627948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP190102048"],"award-info":[{"award-number":["DP190102048"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Aedes aegypti, the yellow fever mosquito, and Aedes albopictus, the Asian tiger mosquito, are the most significant vectors of dengue, Zika, and Chikungunya viruses globally. Studies examining host factors that control arbovirus transmission demonstrate that insect-specific viruses (ISVs) can modulate mosquitoes\u2019 susceptibility to arbovirus infection in both in vivo and in vitro co-infection models. While research is ongoing to implicate individual ISVs as proviral or antiviral factors, we have a limited understanding of the composition and diversity of the Aedes virome. To address this gap, we used a meta-analysis approach to uncover virome diversity by analysing ~3000 available RNA sequencing libraries representing a worldwide geographic range for both mosquitoes. We identified ten novel viruses and previously characterised viruses, including mononegaviruses, orthomyxoviruses, negeviruses, and a novel bi-segmented negev-like group. Phylogenetic analysis suggests close relatedness to mosquito viruses implying likely insect host range except for one arbovirus, the multi-segmented Jingmen tick virus (Flaviviridae) in an Italian colony of Ae. albopictus. Individual mosquito transcriptomes revealed remarkable inter-host variation of ISVs within individuals from the same colony and heterogeneity between different laboratory strains. Additionally, we identified striking virus diversity in Wolbachia infected Aedes cell lines. This study expands our understanding of the virome of these important vectors. It provides a resource for further assessing the ecology, evolution, and interaction of ISVs with their mosquito hosts and the arboviruses they transmit.<\/jats:p>","DOI":"10.3390\/microorganisms9081653","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T02:16:07Z","timestamp":1628043367000},"page":"1653","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Uncovering the Worldwide Diversity and Evolution of the Virome of the Mosquitoes Aedes aegypti and Aedes albopictus"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9238-1952","authenticated-orcid":false,"given":"Rhys","family":"Parry","sequence":"first","affiliation":[{"name":"School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6296-0187","authenticated-orcid":false,"given":"Maddie E","family":"James","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Queensland, Brisbane, QLD 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7885-6544","authenticated-orcid":false,"given":"Sassan","family":"Asgari","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Queensland, Brisbane, QLD 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e08347","DOI":"10.7554\/eLife.08347","article-title":"The global distribution of the arbovirus vectors Aedes aegypti and Ae. albopictus","volume":"4","author":"Kraemer","year":"2015","journal-title":"eLife"},{"key":"ref_2","first-page":"1","article-title":"The biology of Aedes albopictus","volume":"1","author":"Hawley","year":"1988","journal-title":"J. Am. Mosq. Control. Assoc. Suppl."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Li, Y., Kamara, F., Zhou, G., Puthiyakunnon, S., Li, C., Liu, Y., Zhou, Y., Yao, L., Yan, G., and Chen, X.G. (2014). Urbanization increases Aedes albopictus larval habitats and accelerates mosquito development and survivorship. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0003301"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.meegid.2018.11.009","article-title":"Aedes aegypti vector competence studies: A review","volume":"67","author":"Powell","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.0269-283X.2004.00513.x","article-title":"Critical review of the vector status of Aedes albopictus","volume":"18","author":"Gratz","year":"2004","journal-title":"Med. Vet. Entomol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"6294","DOI":"10.1128\/JVI.00370-14","article-title":"High level of vector competence of Aedes aegypti and Aedes albopictus from ten American countries as a crucial factor in the spread of Chikungunya virus","volume":"88","author":"Zouache","year":"2014","journal-title":"J. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Bouzid, M., Colon-Gonzalez, F.J., Lung, T., Lake, I.R., and Hunter, P.R. (2014). Climate change and the emergence of vector-borne diseases in Europe: Case study of dengue fever. BMC Public Health, 14.","DOI":"10.1186\/1471-2458-14-781"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Lambrechts, L., Scott, T.W., and Gubler, D.J. (2010). Consequences of the expanding global distribution of Aedes albopictus for dengue virus transmission. PLoS Negl. Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000646"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.cll.2010.01.001","article-title":"Yellow fever: A reemerging threat","volume":"30","author":"Gardner","year":"2010","journal-title":"Clin. Lab. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1128\/CMR.00072-15","article-title":"Zika Virus","volume":"29","author":"Musso","year":"2016","journal-title":"Clin. Microbiol Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/nature22402","article-title":"Zika virus evolution and spread in the Americas","volume":"546","author":"Metsky","year":"2017","journal-title":"Nature"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1186\/s12985-017-0772-y","article-title":"Zika vector transmission risk in temperate Australia: A vector competence study","volume":"14","author":"Duchemin","year":"2017","journal-title":"Virol. J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S1473-3099(01)00016-0","article-title":"Yellow fever: An update","volume":"1","author":"Monath","year":"2001","journal-title":"Lancet Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.coviro.2015.10.003","article-title":"Dissecting vectorial capacity for mosquito-borne viruses","volume":"15","author":"Kramer","year":"2015","journal-title":"Curr. Opin. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.ibmb.2019.04.004","article-title":"Expanding the canon: Non-classical mosquito genes at the interface of arboviral infection","volume":"109","author":"Sigle","year":"2019","journal-title":"Insect. Biochem. Mol. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.coviro.2015.08.011","article-title":"The microbiome modulates arbovirus transmission in mosquitoes","volume":"15","author":"Hegde","year":"2015","journal-title":"Curr. Opin. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e00224-18","DOI":"10.1128\/JVI.00224-18","article-title":"Aedes Anphevirus: An Insect-Specific Virus Distributed Worldwide in Aedes aegypti Mosquitoes That Has Complex Interplays with Wolbachia and Dengue Virus Infection in Cells","volume":"92","author":"Parry","year":"2018","journal-title":"J. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6935","DOI":"10.1038\/s41598-017-07279-5","article-title":"Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines","volume":"7","author":"Zhang","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Hobson-Peters, J., Yam, A.W., Lu, J.W., Setoh, Y.X., May, F.J., Kurucz, N., Walsh, S., Prow, N.A., Davis, S.S., and Weir, R. (2013). A new insect-specific flavivirus from northern Australia suppresses replication of West Nile virus and Murray Valley encephalitis virus in co-infected mosquito cells. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0056534"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1186\/s13071-016-1683-2","article-title":"The insect-specific Palm Creek virus modulates West Nile virus infection in and transmission by Australian mosquitoes","volume":"9","author":"McLean","year":"2016","journal-title":"Parasit Vectors"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.virol.2015.05.009","article-title":"Eilat virus induces both homologous and heterologous interference","volume":"484","author":"Nasar","year":"2015","journal-title":"Virology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e00705-19","DOI":"10.1128\/JVI.00705-19","article-title":"Cell-Fusing Agent Virus Reduces Arbovirus Dissemination in Aedes aegypti Mosquitoes In Vivo","volume":"93","author":"Baidaliuk","year":"2019","journal-title":"J. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e00736","DOI":"10.1016\/j.heliyon.2018.e00736","article-title":"Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses","volume":"4","author":"Fujita","year":"2018","journal-title":"Heliyon"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1590\/S0074-02762011000300006","article-title":"Genetic and biological characterization of a densovirus isolate that affects dengue virus infection","volume":"106","author":"Mosimann","year":"2011","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.virol.2018.03.022","article-title":"Dual Insect specific virus infection limits Arbovirus replication in Aedes mosquito cells","volume":"518","author":"Schultz","year":"2018","journal-title":"Virology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e00680-17","DOI":"10.1128\/JVI.00680-17","article-title":"High-Resolution Metatranscriptomics Reveals the Ecological Dynamics of Mosquito-Associated RNA Viruses in Western Australia","volume":"91","author":"Shi","year":"2017","journal-title":"J. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Webster, C.L., Waldron, F.M., Robertson, S., Crowson, D., Ferrari, G., Quintana, J.F., Brouqui, J.M., Bayne, E.H., Longdon, B., and Buck, A.H. (2015). The Discovery, Distribution, and Evolution of Viruses Associated with Drosophila melanogaster. PLoS Biol., 13.","DOI":"10.1101\/021154"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.virol.2018.12.020","article-title":"A reverse-transcription\/RNase H based protocol for depletion of mosquito ribosomal RNA facilitates viral intrahost evolution analysis, transcriptomics and pathogen discovery","volume":"528","author":"Fauver","year":"2018","journal-title":"Virology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3389\/fmicb.2015.00185","article-title":"RNA shotgun metagenomic sequencing of northern California (USA) mosquitoes uncovers viruses, bacteria, and fungi","volume":"6","author":"Chandler","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Sabin, L.R., Zheng, Q., Thekkat, P., Yang, J., Hannon, G.J., Gregory, B.D., Tudor, M., and Cherry, S. (2013). Dicer-2 processes diverse viral RNA species. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0055458"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"13729","DOI":"10.1128\/JVI.02041-12","article-title":"RNA interference as a cellular defense mechanism against the DNA virus baculovirus","volume":"86","author":"Jayachandran","year":"2012","journal-title":"J. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"702","DOI":"10.3390\/genes3040702","article-title":"RNAi in Arthropods: Insight into the Machinery and Applications for Understanding the Pathogen-Vector Interface","volume":"3","author":"Barnard","year":"2012","journal-title":"Genes"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1073\/pnas.0911353107","article-title":"Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAs","volume":"107","author":"Wu","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"6191","DOI":"10.1093\/nar\/gkv587","article-title":"Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host","volume":"43","author":"Aguiar","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1093\/bioinformatics\/btl158","article-title":"Cd-hit: A fast program for clustering and comparing large sets of protein or nucleotide sequences","volume":"22","author":"Li","year":"2006","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and applications. BMC Bioinform., 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Katzourakis, A., and Gifford, R.J. (2010). Endogenous viral elements in animal genomes. PLoS Genet., 6.","DOI":"10.1371\/journal.pgen.1001191"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e02124-18","DOI":"10.1128\/JVI.02124-18","article-title":"Endogenous Viral Elements Are Widespread in Arthropod Genomes and Commonly Give Rise to PIWI-Interacting RNAs","volume":"93","author":"Nigg","year":"2019","journal-title":"J. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/S0968-0004(98)01298-5","article-title":"Iterated profile searches with PSI-BLAST\u2013A tool for discovery in protein databases","volume":"23","author":"Altschul","year":"1998","journal-title":"Trends Biochem. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"10171","DOI":"10.1073\/pnas.0900604106","article-title":"Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs","volume":"106","author":"Kosugi","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1093\/bioinformatics\/btp698","article-title":"Fast and accurate long-read alignment with Burrows-Wheeler transform","volume":"26","author":"Li","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1093\/bib\/bbs017","article-title":"Integrative Genomics Viewer (IGV): High-performance genomics data visualization and exploration","volume":"14","author":"Thorvaldsdottir","year":"2013","journal-title":"Brief. Bioinform."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: A flexible suite of utilities for comparing genomic features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Parry, R., Naccache, F., Ndiaye, E.H., Fall, G., Castelli, I., Luhken, R., Medlock, J., Cull, B., Hesson, J.C., and Montarsi, F. (2020). Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes. Viruses, 12.","DOI":"10.3390\/v12040440"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: Multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1093\/bioinformatics\/btp348","article-title":"trimAl: A tool for automated alignment trimming in large-scale phylogenetic analyses","volume":"25","author":"Gabaldon","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/molbev\/msu300","article-title":"IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies","volume":"32","author":"Nguyen","year":"2015","journal-title":"Mol. Biol. Evol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1093\/molbev\/msn067","article-title":"An improved general amino acid replacement matrix","volume":"25","author":"Le","year":"2008","journal-title":"Mol. Biol. Evol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1093\/molbev\/mst024","article-title":"Ultrafast approximation for phylogenetic bootstrap","volume":"30","author":"Minh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1038\/nature13964","article-title":"Evolution of mosquito preference for humans linked to an odorant receptor","volume":"515","author":"McBride","year":"2014","journal-title":"Nature"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.virol.2018.07.029","article-title":"Virome of >12 thousand Culex mosquitoes from throughout California","volume":"523","author":"Sadeghi","year":"2018","journal-title":"Virology"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3766","DOI":"10.1128\/JVI.03483-13","article-title":"Common mechanism for RNA encapsidation by negative-strand RNA viruses","volume":"88","author":"Green","year":"2014","journal-title":"J. Virol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1534\/g3.119.401006","article-title":"The Developmental Transcriptome of Ae. albopictus, a Major Worldwide Human Disease Vector","volume":"10","author":"Gamez","year":"2020","journal-title":"G3"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1101\/gr.265157.120","article-title":"A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons","volume":"31","author":"Ma","year":"2021","journal-title":"Genome Res."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Frangeul, L., Blanc, H., Saleh, M.C., and Suzuki, Y. (2020). Differential Small RNA Responses against Co-Infecting Insect-Specific Viruses in Aedes albopictus Mosquitoes. Viruses, 12.","DOI":"10.1101\/2020.03.28.012062"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3389\/fcimb.2018.00359","article-title":"Metagenomic Analysis of Flaviviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes From Dengue and Zika Viruses","volume":"8","author":"Xiao","year":"2018","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Kubacki, J., Flacio, E., Qi, W., Guidi, V., Tonolla, M., and Fraefel, C. (2020). Viral Metagenomic Analysis of Aedes albopictus Mosquitos from Southern Switzerland. Viruses, 12.","DOI":"10.3390\/v12090929"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1038\/ismej.2013.192","article-title":"Integrated transcriptomic and proteomic analysis of the global response of Wolbachia to doxycycline-induced stress","volume":"8","author":"Darby","year":"2014","journal-title":"ISME J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1111\/mmi.14668","article-title":"Altered gene expression profile of Wolbachia pipientis wAlbB strain following transinfection from its native host Aedes albopictus to Aedes aegypti cells","volume":"115","author":"Bishop","year":"2020","journal-title":"Mol. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Woolfit, M., Algama, M., Keith, J.M., McGraw, E.A., and Popovici, J. (2015). Discovery of putative small non-coding RNAs from the obligate intracellular bacterium Wolbachia pipientis. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0118595"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e00581-19","DOI":"10.1128\/JVI.00581-19","article-title":"Infection of Aedes albopictus Mosquito C6\/36 Cells with the wMelpop Strain of Wolbachia Modulates Dengue Virus-Induced Host Cellular Transcripts and Induces Critical Sequence Alterations in the Dengue Viral Genome","volume":"93","author":"Teramoto","year":"2019","journal-title":"J. Virol."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Mayoral, J.G., Etebari, K., Hussain, M., Khromykh, A.A., and Asgari, S. (2014). Wolbachia infection modifies the profile, shuttling and structure of microRNAs in a mosquito cell line. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0096107"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"18721","DOI":"10.1073\/pnas.1420131112","article-title":"Wolbachia small noncoding RNAs and their role in cross-kingdom communications","volume":"111","author":"Mayoral","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Xu, J.B., Su, X.H., Bonizzoni, M., Zhong, D.B., Li, Y.J., Zhou, G.F., Nguyen, H., Tong, S., Yan, G.Y., and Chen, X.G. (2018). Comparative transcriptome analysis and RNA interference reveal CYP6A8 and SNPs related to pyrethroid resistance in Aedes albopictus. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006828"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"e5907","DOI":"10.1073\/pnas.1516410112","article-title":"Genome sequence of the Asian Tiger mosquito, Aedes albopictus, reveals insights into its biology, genetics, and evolution","volume":"112","author":"Chen","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1126\/science.aaa2850","article-title":"Sex Determination. A male-determining factor in the mosquito Aedes aegypti","volume":"348","author":"Hall","year":"2015","journal-title":"Science"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Lombardo, F., Salvemini, M., Fiorillo, C., Nolan, T., Zwiebel, L.J., Ribeiro, J.M., and Arca, B. (2017). Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus. BMC Genom., 18.","DOI":"10.1186\/s12864-017-4144-1"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Vedururu, R.K., Neave, M.J., Tachedjian, M., Klein, M.J., Gorry, P.R., Duchemin, J.B., and Paradkar, P.N. (2019). RNASeq Analysis of Aedes albopictus Mosquito Midguts after Chikungunya Virus Infection. Viruses, 11.","DOI":"10.3390\/v11060513"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/nature20167","article-title":"Redefining the invertebrate RNA virosphere","volume":"540","author":"Shi","year":"2016","journal-title":"Nature"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/s41559-017-0403-4","article-title":"Pan-arthropod analysis reveals somatic piRNAs as an ancestral defence against transposable elements","volume":"2","author":"Lewis","year":"2018","journal-title":"Nat. Ecol. Evol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1186\/s13071-016-1634-y","article-title":"Larval application of sodium channel homologous dsRNA restores pyrethroid insecticide susceptibility in a resistant adult mosquito population","volume":"9","author":"Bona","year":"2016","journal-title":"Parasit Vectors"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"14899","DOI":"10.1038\/s41598-020-71904-z","article-title":"Mating and blood-feeding induce transcriptome changes in the spermathecae of the yellow fever mosquito Aedes aegypti","volume":"10","author":"Camargo","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Zhao, L., Alto, B.W., Shin, D., and Yu, F. (2018). The Effect of Permethrin Resistance on Aedes aegypti Transcriptome Following Ingestion of Zika Virus Infected Blood. Viruses, 10.","DOI":"10.3390\/v10090470"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"16087","DOI":"10.1038\/nmicrobiol.2016.87","article-title":"Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes","volume":"1","author":"Liu","year":"2016","journal-title":"Nat. Microbiol."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Ribeiro, J.M., Martin-Martin, I., Arca, B., and Calvo, E. (2016). A Deep Insight into the Sialome of Male and Female Aedes aegypti Mosquitoes. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0151400"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Kang, D.S., Barron, M.S., Lovin, D.D., Cunningham, J.M., Eng, M.W., Chadee, D.D., Li, J., and Severson, D.W. (2018). A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006568"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"4690","DOI":"10.1038\/s41598-018-22945-y","article-title":"Mapping the virome in wild-caught Aedes aegypti from Cairns and Bangkok","volume":"8","author":"Zakrzewski","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"e00316-20","DOI":"10.1128\/mSphere.00316-20","article-title":"Under-the-Radar Dengue Virus Infections in Natural Populations of Aedes aegypti Mosquitoes","volume":"5","author":"Boyles","year":"2020","journal-title":"mSphere"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Huang, X., Poelchau, M.F., and Armbruster, P.A. (2015). Global Transcriptional Dynamics of Diapause Induction in Non-Blood-Fed and Blood-Fed Aedes albopictus. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003724"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Tsujimoto, H., Hanley, K.A., Sundararajan, A., Devitt, N.P., Schilkey, F.D., and Hansen, I.A. (2018). Dengue virus serotype 2 infection alters midgut and carcass gene expression in the Asian tiger mosquito, Aedes albopictus. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0192128"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Esquivel, C.J., Cassone, B.J., and Piermarini, P.M. (2014). Transcriptomic evidence for a dramatic functional transition of the malpighian tubules after a blood meal in the Asian tiger mosquito Aedes albopictus. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002929"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.chom.2018.02.001","article-title":"Dicer-2-Dependent Generation of Viral DNA from Defective Genomes of RNA Viruses Modulates Antiviral Immunity in Insects","volume":"23","author":"Poirier","year":"2018","journal-title":"Cell Host Microbe"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1111\/imb.12332","article-title":"MicroRNAs are differentially abundant during Aedes albopictus diapause maintenance but not diapause induction","volume":"26","author":"Batz","year":"2017","journal-title":"Insect. Mol. Biol."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Poelchau, M.F., Reynolds, J.A., Denlinger, D.L., Elsik, C.G., and Armbruster, P.A. (2011). A de novo transcriptome of the Asian tiger mosquito, Aedes albopictus, to identify candidate transcripts for diapause preparation. BMC Genom., 12.","DOI":"10.1186\/1471-2164-12-619"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1111\/phen.12016","article-title":"Transcriptome sequencing as a platform to elucidate molecular components of the diapause response in the Asian tiger mosquito Aedes albopictus","volume":"38","author":"Poelchau","year":"2013","journal-title":"Physiol. Entomol."},{"key":"ref_90","first-page":"20130143","article-title":"Deep sequencing reveals complex mechanisms of diapause preparation in the invasive mosquito, Aedes albopictus","volume":"280","author":"Poelchau","year":"2013","journal-title":"Proc. Biol. Sci."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"18404","DOI":"10.1038\/s41598-020-75042-4","article-title":"Experimental adaptation of dengue virus 1 to Aedes albopictus mosquitoes by in vivo selection","volume":"10","author":"Bellone","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Adelman, Z.N., Anderson, M.A., Wiley, M.R., Murreddu, M.G., Samuel, G.H., Morazzani, E.M., and Myles, K.M. (2013). Cooler temperatures destabilize RNA interference and increase susceptibility of disease vector mosquitoes to viral infection. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002239"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1186\/s13059-020-02141-w","article-title":"Improved reference genome of the arboviral vector Aedes albopictus","volume":"21","author":"Palatini","year":"2020","journal-title":"Genome Biol."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Marconcini, M., Pischedda, E., Hou\u00e9, V., Palatini, U., Lozada-Ch\u00e1vez, N., Sogliani, D., Failloux, A.B., and Bonizzoni, M. (2021). Profile of Small RNAs, vDNA Forms and Viral Integrations in Late Chikungunya Virus Infection of Aedes albopictus Mosquitoes. Viruses, 13.","DOI":"10.3390\/v13040553"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"975","DOI":"10.3389\/fgene.2019.00975","article-title":"Differentiation of Long Non-Coding RNA and mRNA Expression Profiles in Male and Female Aedes albopictus","volume":"10","author":"Xu","year":"2019","journal-title":"Front. Genet."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1111\/j.1365-2583.2012.01141.x","article-title":"Sindbis virus induces the production of a novel class of endogenous siRNAs in Aedes aegypti mosquitoes","volume":"21","author":"Adelman","year":"2012","journal-title":"Insect. Mol. Biol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1111\/imb.12152","article-title":"MicroRNAs of two medically important mosquito species: Aedes aegypti and Anopheles stephensi","volume":"24","author":"Hu","year":"2015","journal-title":"Insect. Mol. Biol."},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Biedler, J.K., Hu, W., Tae, H., and Tu, Z. (2012). Identification of early zygotic genes in the yellow fever mosquito Aedes aegypti and discovery of a motif involved in early zygotic genome activation. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0033933"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1093\/gbe\/evv115","article-title":"Complete Dosage Compensation in Anopheles stephensi and the Evolution of Sex-Biased Genes in Mosquitoes","volume":"7","author":"Jiang","year":"2015","journal-title":"Genome Biol. Evol."},{"key":"ref_100","first-page":"e7738","article-title":"Juvenile hormone-regulated alternative splicing of the taiman gene primes the ecdysteroid response in adult mosquitoes","volume":"115","author":"Liu","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Choi, Y.J., Aliota, M.T., Mayhew, G.F., Erickson, S.M., and Christensen, B.M. (2014). Dual RNA-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002905"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"e01070-20","DOI":"10.1128\/JVI.01070-20","article-title":"Partitiviruses Infecting Drosophila melanogaster and Aedes aegypti Exhibit Efficient Biparental Vertical Transmission","volume":"94","author":"Cross","year":"2020","journal-title":"J. Virol."},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Bonizzoni, M., Dunn, W.A., Campbell, C.L., Olson, K.E., Marinotti, O., and James, A.A. (2012). Complex modulation of the Aedes aegypti transcriptome in response to dengue virus infection. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0050512"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"3656","DOI":"10.1073\/pnas.1810771116","article-title":"Engineered resistance to Zika virus in transgenic Aedes aegypti expressing a polycistronic cluster of synthetic small RNAs","volume":"116","author":"Buchman","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"15968","DOI":"10.1038\/srep15968","article-title":"Dengue virus infection alters post-transcriptional modification of microRNAs in the mosquito vector Aedes aegypti","volume":"5","author":"Etebari","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1038\/s41467-020-16086-y","article-title":"Zika virus noncoding RNA suppresses apoptosis and is required for virus transmission by mosquitoes","volume":"11","author":"Slonchak","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"200246","DOI":"10.1098\/rsob.200246","article-title":"Temperature modulates immune gene expression in mosquitoes during arbovirus infection","volume":"11","author":"Barrero","year":"2021","journal-title":"Open Biol."},{"key":"ref_108","first-page":"3141","article-title":"The Transcriptional Response of Aedes aegypti with Variable Extrinsic Incubation Periods for Dengue Virus","volume":"10","author":"Koh","year":"2018","journal-title":"Genome Biol. Evol."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"e00456-17","DOI":"10.1128\/mSphere.00456-17","article-title":"Global Transcriptome Analysis of Aedes aegypti Mosquitoes in Response to Zika Virus Infection","volume":"2","author":"Etebari","year":"2017","journal-title":"mSphere"},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Saldana, M.A., Etebari, K., Hart, C.E., Widen, S.G., Wood, T.G., Thangamani, S., Asgari, S., and Hughes, G.L. (2017). Zika virus alters the microRNA expression profile and elicits an RNAi response in Aedes aegypti mosquitoes. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005760"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"901","DOI":"10.3389\/fmicb.2020.00901","article-title":"Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions","volume":"11","author":"Ferreira","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1099\/jgv.0.000832","article-title":"Understanding the role of microRNAs in the interaction of Aedes aegypti mosquitoes with an insect-specific flavivirus","volume":"98","author":"Lee","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1111\/eva.13177","article-title":"A genomic amplification affecting a carboxylesterase gene cluster confers organophosphate resistance in the mosquito Aedes aegypti: From genomic characterization to high-throughput field detection","volume":"14","author":"Cattel","year":"2021","journal-title":"Evol. Appl."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.bbrc.2017.01.162","article-title":"Transcriptome analysis of Aedes aegypti in response to mono-infections and co-infections of dengue virus-2 and chikungunya virus","volume":"492","author":"Shrinet","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Chowdhury, A., Modahl, C.M., Tan, S.T., Wong Wei Xiang, B., Misse, D., Vial, T., Kini, R.M., and Pompon, J.F. (2020). JNK pathway restricts DENV2, ZIKV and CHIKV infection by activating complement and apoptosis in mosquito salivary glands. PLoS Pathog., 16.","DOI":"10.1371\/journal.ppat.1008754"},{"key":"ref_116","doi-asserted-by":"crossref","unstructured":"Choy, M.M., Sessions, O.M., Gubler, D.J., and Ooi, E.E. (2015). Production of Infectious Dengue Virus in Aedes aegypti Is Dependent on the Ubiquitin Proteasome Pathway. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0004227"},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1038\/s41586-020-2159-2","article-title":"A satellite repeat-derived piRNA controls embryonic development of Aedes","volume":"580","author":"Halbach","year":"2020","journal-title":"Nature"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1074\/mcp.RA118.001067","article-title":"Proteins, Transcripts, and Genetic Architecture of Seminal Fluid and Sperm in the Mosquito Aedes aegypti","volume":"18","author":"Degner","year":"2019","journal-title":"Mol. Cell Proteom."},{"key":"ref_119","doi-asserted-by":"crossref","unstructured":"Raquin, V., Merkling, S.H., Gausson, V., Moltini-Conclois, I., Frangeul, L., Varet, H., Dillies, M.A., Saleh, M.C., and Lambrechts, L. (2017). Individual co-variation between viral RNA load and gene expression reveals novel host factors during early dengue virus infection of the Aedes aegypti midgut. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0006152"},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Eng, M.W., Clemons, A., Hill, C., Engel, R., Severson, D.W., and Behura, S.K. (2018). Multifaceted functional implications of an endogenously expressed tRNA fragment in the vector mosquito Aedes aegypti. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006186"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1186\/s40168-019-0734-2","article-title":"Stable distinct core eukaryotic viromes in different mosquito species from Guadeloupe, using single mosquito viral metagenomics","volume":"7","author":"Shi","year":"2019","journal-title":"Microbiome"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1007\/s00705-019-04247-4","article-title":"Taxonomy of the order Mononegavirales: Update 2019","volume":"164","author":"Amarasinghe","year":"2019","journal-title":"Arch. Virol."},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Vedururu, R.K., Neave, M.J., Sundaramoorthy, V., Green, D., Harper, J.A., Gorry, P.R., Duchemin, J.B., and Paradkar, P.N. (2019). Whole Transcriptome Analysis of Aedes albopictus Mosquito Head and Thorax Post-Chikungunya Virus Infection. Pathogens, 8.","DOI":"10.3390\/pathogens8030132"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1128\/JVI.00776-12","article-title":"Negevirus: A proposed new taxon of insect-specific viruses with wide geographic distribution","volume":"87","author":"Vasilakis","year":"2013","journal-title":"J. Virol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"4346","DOI":"10.3390\/v6114346","article-title":"Genetic characterization of goutanap virus, a novel virus related to negeviruses, cileviruses and higreviruses","volume":"6","author":"Kallies","year":"2014","journal-title":"Viruses"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.virol.2016.07.031","article-title":"West African Anopheles gambiae mosquitoes harbor a taxonomically diverse virome including new insect-specific flaviviruses, mononegaviruses, and totiviruses","volume":"498","author":"Fauver","year":"2016","journal-title":"Virology"},{"key":"ref_127","first-page":"13","article-title":"Twenty-Five New Viruses Associated with the Drosophilidae (Diptera)","volume":"12","author":"Webster","year":"2016","journal-title":"Evol Bioinform Online"},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1099\/jgv.0.000884","article-title":"ICTV Virus Taxonomy Profile: Virgaviridae","volume":"98","author":"Adams","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_129","doi-asserted-by":"crossref","unstructured":"Morazzani, E.M., Wiley, M.R., Murreddu, M.G., Adelman, Z.N., and Myles, K.M. (2012). Production of virus-derived ping-pong-dependent piRNA-like small RNAs in the mosquito soma. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1002470"},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"e00639-16","DOI":"10.1128\/genomeA.00639-16","article-title":"Complete Genome Sequence of Diaphorina citri-associated C virus, a Novel Putative RNA Virus of the Asian Citrus Psyllid, Diaphorina citri","volume":"4","author":"Nouri","year":"2016","journal-title":"Genome Announc."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/JVI.02595-13","article-title":"Powerful sequence similarity search methods and in-depth manual analyses can identify remote homologs in many apparently \u201corphan\u201d viral proteins","volume":"88","author":"Kuchibhatla","year":"2014","journal-title":"J. Virol."},{"key":"ref_132","doi-asserted-by":"crossref","unstructured":"Cook, S., Chung, B.Y., Bass, D., Moureau, G., Tang, S., McAlister, E., Culverwell, C.L., Glucksman, E., Wang, H., and Brown, T.D. (2013). Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0080720"},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.cois.2018.05.009","article-title":"Densonucleosis viruses (\u2018densoviruses\u2019) for mosquito and pathogen control","volume":"28","author":"Johnson","year":"2018","journal-title":"Curr. Opin. Insect. Sci."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1099\/jgv.0.001212","article-title":"ICTV Virus Taxonomy Profile: Parvoviridae","volume":"100","author":"Cotmore","year":"2019","journal-title":"J. Gen. Virol."},{"key":"ref_135","doi-asserted-by":"crossref","unstructured":"Ren, X., Hoiczyk, E., and Rasgon, J.L. (2008). Viral paratransgenesis in the malaria vector Anopheles gambiae. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000135"},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.virusres.2017.03.005","article-title":"Analysis of the miRNA profile in C6\/36 cells persistently infected with dengue virus type 2","volume":"232","author":"Marchat","year":"2017","journal-title":"Virus Res."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.3389\/fmicb.2019.01549","article-title":"A Novel Densovirus Isolated From the Asian Tiger Mosquito Displays Varied Pathogenicity Depending on Its Host Species","volume":"10","author":"Li","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"6744","DOI":"10.1073\/pnas.1324194111","article-title":"A tick-borne segmented RNA virus contains genome segments derived from unsegmented viral ancestors","volume":"111","author":"Qin","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.ebiom.2019.04.004","article-title":"Emergence of human infection with Jingmen tick virus in China: A retrospective study","volume":"43","author":"Jia","year":"2019","journal-title":"EBioMedicine"},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.meegid.2018.07.010","article-title":"Viral metagenomics, genetic and evolutionary characteristics of Crimean-Congo hemorrhagic fever orthonairovirus in humans, Kosovo","volume":"65","author":"Emmerich","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"ref_141","doi-asserted-by":"crossref","unstructured":"Dittmer, J., Alafndi, A., and Gabrieli, P. (2019). Fat body-specific vitellogenin expression regulates host-seeking behaviour in the mosquito Aedes albopictus. PLoS Biol., 17.","DOI":"10.1371\/journal.pbio.3000238"},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"e00645-19","DOI":"10.1128\/mSphere.00645-19","article-title":"Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses","volume":"4","author":"Temmam","year":"2019","journal-title":"mSphere"},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"10421","DOI":"10.1038\/s41598-019-46914-1","article-title":"Viral Diversity of Tick Species Parasitizing Cattle and Dogs in Trinidad and Tobago","volume":"9","author":"Sameroff","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.ibmb.2019.04.008","article-title":"Small RNA responses of Culex mosquitoes and cell lines during acute and persistent virus infection","volume":"109","author":"Ruckert","year":"2019","journal-title":"Insect. Biochem. Mol. Biol."},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"e68353","DOI":"10.7554\/eLife.68353","article-title":"Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay","volume":"10","author":"Batson","year":"2021","journal-title":"eLife"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"e00640-20","DOI":"10.1128\/mSystems.00640-20","article-title":"Stability of the Virome in Lab- and Field-Collected Aedes albopictus Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of Aedes Mosquitoes","volume":"5","author":"Shi","year":"2020","journal-title":"mSystems"},{"key":"ref_147","doi-asserted-by":"crossref","unstructured":"Brackney, D.E., Scott, J.C., Sagawa, F., Woodward, J.E., Miller, N.A., Schilkey, F.D., Mudge, J., Wilusz, J., Olson, K.E., and Blair, C.D. (2010). C6\/36 Aedes albopictus cells have a dysfunctional antiviral RNA interference response. PLoS Negl. Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000856"},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.coviro.2018.08.008","article-title":"Expansion of the metazoan virosphere: Progress, pitfalls, and prospects","volume":"31","author":"Obbard","year":"2018","journal-title":"Curr. Opin. Virol."},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1603\/ME10152","article-title":"Early history of laboratory breeding of Aedes aegypti (Diptera: Culicidae) focusing on the origins and use of selected strains","volume":"47","author":"Kuno","year":"2010","journal-title":"J. Med. Entomol."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"399","DOI":"10.4269\/ajtmh.1991.45.399","article-title":"Genetic selection of a flavivirus-refractory strain of the yellow fever mosquito Aedes aegypti","volume":"45","author":"Miller","year":"1991","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.virol.2018.06.007","article-title":"Adventitious viruses persistently infect three commonly used mosquito cell lines","volume":"521","author":"Ruckert","year":"2018","journal-title":"Virology"},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/0042-6822(75)90113-0","article-title":"An agent in the Aedes aegypti cell line (Peleg) which causes fusion of Aedes albopictus cells","volume":"64","author":"Stollar","year":"1975","journal-title":"Virology"},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/0042-6822(76)90139-2","article-title":"Morphological, Biochemical, and Serological Studies on a Viral Agent (Cfa) Which Replicates in and Causes Fusion of Aedes-Albopictus (Singh) Cells","volume":"74","author":"Igarashi","year":"1976","journal-title":"Virology"},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/0042-6822(92)90575-A","article-title":"The complete nucleotide sequence of cell fusing agent (CFA): Homology between the nonstructural proteins encoded by CFA and the nonstructural proteins encoded by arthropod-borne flaviviruses","volume":"189","author":"Cisar","year":"1992","journal-title":"Virology"},{"key":"ref_155","doi-asserted-by":"crossref","unstructured":"Maringer, K., Yousuf, A., Heesom, K.J., Fan, J., Lee, D., Fernandez-Sesma, A., Bessant, C., Matthews, D.A., and Davidson, A.D. (2017). Proteomics informed by transcriptomics for characterising active transposable elements and genome annotation in Aedes aegypti. BMC Genom., 18.","DOI":"10.1186\/s12864-016-3432-5"},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1099\/jgv.0.001079","article-title":"Complete genome of Aedes aegypti anphevirus in the Aag2 mosquito cell line","volume":"99","author":"Audsley","year":"2018","journal-title":"J. Gen. Virol."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.virol.2018.11.012","article-title":"Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines","volume":"527","author":"McLean","year":"2019","journal-title":"Virology"},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1099\/jgv.0.001173","article-title":"Detection, infection dynamics and small RNA response against Culex Y virus in mosquito-derived cells","volume":"99","author":"Franzke","year":"2018","journal-title":"J. Gen. Virol."},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1099\/0022-1317-76-8-2067","article-title":"Insect densoviruses may be widespread in mosquito cell lines","volume":"76","author":"Kittayapong","year":"1995","journal-title":"J. Gen. Virol."},{"key":"ref_160","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/0168-1702(93)90052-O","article-title":"A parvo-like virus persistently infecting a C6\/36 clone of Aedes albopictus mosquito cell line and pathogenic for Aedes aegypti larvae","volume":"29","author":"Jousset","year":"1993","journal-title":"Virus Res."},{"key":"ref_161","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1006\/viro.1994.1239","article-title":"Complete nucleotide sequence and genomic organization of the Aedes albopictus parvovirus (AaPV) pathogenic for Aedes aegypti larvae","volume":"200","author":"Boublik","year":"1994","journal-title":"Virology"},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1099\/0022-1317-40-3-531","article-title":"Isolation of a singhs Aedes albopictus cell Clone sensitive to Dengue and chikungunya viruses","volume":"40","author":"Igarashi","year":"1978","journal-title":"J. Gen. Virol."},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.virol.2018.12.006","article-title":"Density-dependent enhanced replication of a densovirus in Wolbachia-infected Aedes cells is associated with production of piRNAs and higher virus-derived siRNAs","volume":"528","author":"Parry","year":"2019","journal-title":"Virology"},{"key":"ref_164","doi-asserted-by":"crossref","unstructured":"Skalsky, R.L., Vanlandingham, D.L., Scholle, F., Higgs, S., and Cullen, B.R. (2010). Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus. BMC Genom., 11.","DOI":"10.1186\/1471-2164-11-119"},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"e00339-17","DOI":"10.1128\/JVI.00339-17","article-title":"Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures","volume":"91","author":"Schultz","year":"2017","journal-title":"J. Virol."},{"key":"ref_166","doi-asserted-by":"crossref","first-page":"3024","DOI":"10.1099\/jgv.0.000617","article-title":"Wolbachia restricts insect-specific flavivirus infection in Aedes aegypti cells","volume":"97","author":"Schnettler","year":"2016","journal-title":"J. Gen. Virol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/8\/1653\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:39:24Z","timestamp":1760164764000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/8\/1653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,3]]},"references-count":166,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2021,8]]}},"alternative-id":["microorganisms9081653"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms9081653","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,3]]}}}