{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T16:10:17Z","timestamp":1784909417782,"version":"3.55.0"},"reference-count":11,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,9,4]],"date-time":"2021-09-04T00:00:00Z","timestamp":1630713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wroc\u0142aw Medical University in Wroc\u0142aw, Poland","award":["Scientific grant"],"award-info":[{"award-number":["Scientific grant"]}]},{"DOI":"10.13039\/501100015829","name":"Ministerstwo Zdrowia","doi-asserted-by":"publisher","award":["Narodowy Program Ochrony Antybiotyk\u00f3w"],"award-info":[{"award-number":["Narodowy Program Ochrony Antybiotyk\u00f3w"]}],"id":[{"id":"10.13039\/501100015829","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Polish Ministry of Science and Higher Education","award":["SPUB MIKROBANK"],"award-info":[{"award-number":["SPUB MIKROBANK"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Cronobacter sakazakii can cause severe life-threatening invasive infections in neonates, with a high mortality rate mostly associated with powdered infant formula consumption. The study describes a fatal C. sakazakii infection in premature infant fed only with expressed human milk. Despite the identification of etiological factor from patient\u2019s blood, the epidemiological investigation, including mother\u2019s skin, hospital surfaces, milk expressing devices, and milk samples, did not show bacterial contamination. The infection was caused by C. sakazakii ST1, being one of the leading genotypes reported in invasive infections. The phylogenetic analysis of the international collection of the ST1 organisms allowed us to identify the isolate as a member of the main cluster. The pathogenic potential of the isolate was augmented by the presence of IncFIB-like molecule representing virulence plasmids of pESA-3 family. Isolate presented ESBL phenotype associated with blaSHV-12 gene harboured by IncX3 plasmid. The described case gave valuable information to genetics of Cronobacter, and also urges the need of wider whole-genome sequencing implementation as a part of diagnostic procedure.<\/jats:p>","DOI":"10.3390\/microorganisms9091878","type":"journal-article","created":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T13:18:26Z","timestamp":1630934306000},"page":"1878","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Infection of Cronobacter sakazakii ST1 Producing SHV-12 in a Premature Infant Born from Triplet Pregnancy"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8396-0124","authenticated-orcid":false,"given":"Monika","family":"Lachowska","sequence":"first","affiliation":[{"name":"Department of Neonatology, Wroc\u0142aw Medical University, 50-556 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1195-9471","authenticated-orcid":false,"given":"Rados\u0142aw","family":"Izdebski","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, National Medicines Institute, 00-725 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8622-706X","authenticated-orcid":false,"given":"Pawe\u0142","family":"Urbanowicz","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, National Medicines Institute, 00-725 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9386-8885","authenticated-orcid":false,"given":"Dorota","family":"\u017babicka","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Clinical Microbiology, National Medicines Institute, 00-725 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6493-0333","authenticated-orcid":false,"given":"Barbara","family":"Kr\u00f3lak-Olejnik","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Wroc\u0142aw Medical University, 50-556 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2672.2012.05281.x","article-title":"Cronobacter species (formerly known as Enterobacter sakazakii) in powdered infant formula: A review of our current understanding of the biology of this bacterium","volume":"113","author":"Yan","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Jang, H., Gopinath, G.R., Eshwar, A., Srikumar, S., Nguyen, S., Gangiredla, J., Patel, I.R., Finkelstein, S.B., Negrete, F., and Woo, J. (2020). The secretion of toxins and other exoproteins of Cronobacter: Role in virulence, adaption, and persistence. Microorganisms, 8.","DOI":"10.3390\/microorganisms8020229"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"857","DOI":"10.3201\/eid2605.190858","article-title":"Food safety and invasive Cronobacter infections during early infancy, 1961\u20132018","volume":"26","author":"Strysko","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/jp.2013.26","article-title":"Cronobacter: An emerging opportunistic pathogen associated with neonatal meningitis, sepsis and necrotizing enterocolitis","volume":"33","author":"Hunter","year":"2013","journal-title":"J. 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