{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:17:41Z","timestamp":1792095461945,"version":"4.4.0"},"reference-count":56,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T00:00:00Z","timestamp":1631232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Periodontal and Endodontic diseases are biofilm-related diseases. The presence of microorganisms in root canals (RCs) and the complex microbiota of periodontal pockets (PPs) contribute to the development of endodontic-periodontal diseases. This study performed a systemic analysis using state-of-the-art sequence data to assess the microbial composition of infected RCs and PPs to further assess the microbiota and verify the possibility of cross-infection between these sites. The microbiomes of these combined diseases were examined with a focus on the V3-V4 hypervariable region of the 16S rRNA gene. The number of species in PP was higher than in RC, and there was a predominance of obligate anaerobes and gram-negative bacteria. In the RCs, the genera Enterococcus, Parvimonas, Stomatobaculum predominated, in contrast, the PPs revealed a predominance of Enterococcus, Parvimonas, Stomatobaculum, Peptostreptococcus and Mogibacterium. The RC and PP microbiome was not similar with regards to the sharing of OTUs for phyla and genera (8 and 67, respectively). The evaluation of molecular markers revealed a large number of markers for resistance to antibiotics of the carbapenem and beta-lactam type (broad spectrum). Another relevant finding of this study was the markers related to systemic diseases related to cardiac muscle and rheumatology, among others. In conclusion, the RC microbiota was less complex and diverse than PP. Interactions between microbial communities were present. The shared genus can signal communication between the endodontic and periodontal microbiomes.<\/jats:p>","DOI":"10.3390\/microorganisms9091925","type":"journal-article","created":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T21:45:57Z","timestamp":1631483157000},"page":"1925","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Interrelationship between the Microbial Communities of the Root Canals and Periodontal Pockets in Combined Endodontic-Periodontal Diseases"],"prefix":"10.3390","volume":"9","author":[{"given":"Erica M.","family":"Lopes","sequence":"first","affiliation":[{"name":"Department of Restorative Dentistry, Division of Endodontics, Piracicaba Dental School, State University of Campinas -UNICAMP, Piracicaba 13400-001, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maicon R. Z.","family":"Passini","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Division of Endodontics, Piracicaba Dental School, State University of Campinas -UNICAMP, Piracicaba 13400-001, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano T.","family":"Kishi","sequence":"additional","affiliation":[{"name":"National Laboratory of Scientific Computing, Petr\u00f3polis 25715-183, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6689-317X","authenticated-orcid":false,"given":"Tsute","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, The Forsyth Institute, Cambridge, MA 02142, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce J.","family":"Paster","sequence":"additional","affiliation":[{"name":"Microbiology Department, The Forsyth Institute, Cambridge, MA 02142, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda P. F. A.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Division of Endodontics, Piracicaba Dental School, State University of Campinas -UNICAMP, Piracicaba 13400-001, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/prd.12188","article-title":"Interaction between Endodontics and Periodontics","volume":"74","author":"Rotstein","year":"2017","journal-title":"Periodontol 2000"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1016\/j.joen.2015.08.022","article-title":"Microbiomes of Endodontic-Periodontal Lesions before and after Chemomechanical Preparation","volume":"41","author":"Gomes","year":"2015","journal-title":"J. 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