{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:46:26Z","timestamp":1792197986539,"version":"4.4.0"},"reference-count":50,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T00:00:00Z","timestamp":1633910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The skin microbiome and sebum may be associated with inflammation-related diseases of the scalp. To assess the pathogenesis and progression of androgenetic alopecia (AGA), we analyzed the composition of sebum and the bacterial and fungal microbiomes of the scalps of 118 Japanese male individuals with and without AGA, then discussed their roles in the pathogenesis of AGA. Sebum triglyceride and palmitic acid contents were higher in the AGA group than in the non-AGA group. Malassezia restricta, a lipophilic fungus that consumes palmitic acid, was abundant on the scalps of patients with AGA. Cutibacterium, Corynebacterium, and Staphylococcus were the most common genera in both groups, and patients with AGA exhibited scalp dysbiosis (increased abundance of Cutibacterium and decreased abundance of Corynebacterium). Our findings suggest that both sebum and the bacterial and fungal microbiomes of the scalp may be involved in the development of AGA.<\/jats:p>","DOI":"10.3390\/microorganisms9102132","type":"journal-article","created":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T09:16:42Z","timestamp":1633943802000},"page":"2132","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Scalp Microbiome and Sebum Composition in Japanese Male Individuals with and without Androgenetic Alopecia"],"prefix":"10.3390","volume":"9","author":[{"given":"Kazuhiro","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Microbiology, Meiji Pharmaceutical University, Kiyose 204-8588, Japan"},{"name":"Research & Development Headquarters Self-Medication, Taisho Pharmaceutical Co., Ltd., Saitama 331-9530, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mizuna","family":"Inoue","sequence":"additional","affiliation":[{"name":"Research & Development Headquarters Self-Medication, Taisho Pharmaceutical Co., Ltd., Saitama 331-9530, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Otomi","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Meiji Pharmaceutical University, Kiyose 204-8588, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumiko","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Research & Development Headquarters Self-Medication, Taisho Pharmaceutical Co., Ltd., Saitama 331-9530, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Research & Development Headquarters Self-Medication, Taisho Pharmaceutical Co., Ltd., Saitama 331-9530, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Nagahama","sequence":"additional","affiliation":[{"name":"Research & Development Headquarters Self-Medication, Taisho Pharmaceutical Co., Ltd., Saitama 331-9530, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Sugita","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Meiji Pharmaceutical University, Kiyose 204-8588, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/S0190-9622(96)90627-1","article-title":"Guidelines of care for androgenetic alopecia","volume":"35","author":"Drake","year":"1996","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1067\/mjd.2003.81","article-title":"Current and novel methods for assessing efficacy of hair growth promoters in pattern hair loss","volume":"48","author":"Olsen","year":"2003","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.jaad.2004.04.008","article-title":"Evaluation and treatment of male and female pattern hair loss","volume":"52","author":"Olsen","year":"2005","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Orfanos, C.E., Montagna, W., and St\u00fcttgen, G. (1981). Alopecia androgenetica\u2014Its incidence in Japanese and associated conditions. Hair Research, Springer.","DOI":"10.1007\/978-3-642-81650-5"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1111\/j.1365-2133.2003.05565.x","article-title":"Androgenetic alopecia in men aged 40\u201369 years: Prevalence and risk factors","volume":"149","author":"Severi","year":"2003","journal-title":"Br. J. Dermatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"5407","DOI":"10.1038\/s41467-018-07862-y","article-title":"Dissection of gnetic variation and evidence for pleiotropy in male pattern baldness","volume":"9","author":"Yap","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1111\/j.1523-1747.2003.12615.x","article-title":"Genetic basis of male pattern baldness","volume":"121","author":"Nyholt","year":"2003","journal-title":"J. Investig. Dermatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1093\/gerona\/60.8.1077","article-title":"Hair loss among elderly men: Etiology and impact on perceived age","volume":"60","author":"Rexbye","year":"2005","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"794e","DOI":"10.1097\/PRS.0b013e3182865ca9","article-title":"The contribution of endogenous and exogenous factors to male alopecia: A study of identical twins","volume":"131","author":"Gatherwright","year":"2013","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1038\/jid.1969.157","article-title":"Variability in the chemical composition of human skin surface lipids","volume":"53","author":"Downing","year":"1969","journal-title":"J. Investig. Dermatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1111\/1523-1747.ep12277224","article-title":"Age-related changes in sebaceous wax ester secretion rates in men and women","volume":"85","author":"Jacobsen","year":"1985","journal-title":"J. Investig. Dermatol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Grimshaw, S.G., Smith, A.M., Arnold, D.S., Xu, E., Hoptroff, M., and Murphy, B. (2019). The diversity and abundance of fungi and bacteria on the healthy and dandruff affected human scalp. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0225796"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"346","DOI":"10.3389\/fcimb.2018.00346","article-title":"Comparison of healthy and dandruff scalp microbiome reveals the role of commensals in scalp health","volume":"4","author":"Saxena","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1111\/jocd.12736","article-title":"Sensitive scalp is associated with excessive sebum and perturbed microbiome","volume":"18","author":"Ma","year":"2019","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ho, B.S., Ho, E.X.P., Chu, C.W., Ramasamy, S., Bigliardi-Qi, M., de Sessions, P.F., and Bigliardi, P.L. (2019). Microbiome in the hair follicle of androgenetic alopecia patients. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0216330"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1007\/s11046-019-00345-8","article-title":"Investigation on microecology of hair root fungi in androgenetic alopecia patients","volume":"184","author":"Huang","year":"2019","journal-title":"Mycopathologia"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"24877","DOI":"10.1038\/srep24877","article-title":"Dandruff is associated with the conjoined interactions between host and microorganisms","volume":"6","author":"Xu","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.jaad.2003.12.034","article-title":"Skin diseases associated with Malassezia species","volume":"51","author":"Gupta","year":"2004","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"18730","DOI":"10.1073\/pnas.0706756104","article-title":"Dandruff-associated Malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens","volume":"104","author":"Xu","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.1038\/jid.2009.46","article-title":"Involvement of Propionibacterium acnes in the augmentation of lipogenesis in hamster sebaceous glands in vivo and in vitro","volume":"129","author":"Iinuma","year":"2009","journal-title":"J. Investig. Dermatol."},{"key":"ref_21","first-page":"101","article-title":"Development of patterned alopecia","volume":"2","author":"Ogata","year":"1953","journal-title":"Sogo Rinsho"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3486","DOI":"10.1128\/JCM.39.10.3486-3490.2001","article-title":"Molecular analysis of Malassezia microflora on the skin of atopic dermatitis patients and healthy subjects","volume":"39","author":"Sugita","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1093\/mmy\/myaa067","article-title":"The skin mycobiome of an astronaut during a 1-year stay on the International Space Station","volume":"59","author":"Sugita","year":"2021","journal-title":"Med. Mycol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: High-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1038\/ismej.2017.119","article-title":"Exact sequence variants should replace operational taxonomic units in marker-gene data analysis","volume":"11","author":"Callahan","year":"2017","journal-title":"ISME J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1093\/nar\/gkf436","article-title":"MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform","volume":"30","author":"Katoh","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Price, M.N., Dehal, P.S., and Arkin, A.P. (2010). FastTree 2\u2013approximately maximum-likelihood trees for large alignments. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0009490"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1111\/j.1348-0421.2006.tb03825.x","article-title":"Quantitative analysis of cutaneous Malassezia in atopic dermatitis patients using real-time PCR","volume":"50","author":"Sugita","year":"2006","journal-title":"Microbiol. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1111\/bjd.18230","article-title":"The role of the skin microbiota in acne pathophysiology","volume":"181","author":"Ramasamy","year":"2019","journal-title":"Br. J. Dermatol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/j.jaad.2019.06.024","article-title":"Psoriatic lesions are characterized by higher bacterial load and imbalance between Cutibacterium and Corynebacterium","volume":"82","author":"Quan","year":"2020","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Rozas, M., Hart de Ruijter, A., Fabrega, M.J., Zorgani, A., Guell, M., Paetzold, B., and Brillet, F. (2021). From dysbiosis to healthy skin: Major contributions of Cutibacterium acnes to skin homeostasis. Microorganisms, 9.","DOI":"10.3390\/microorganisms9030628"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Pinto, D., Sorbellini, E., Marzani, B., Rucco, M., Giuliani, G., and Rinaldi, F. (2019). Scalp bacterial shift in alopecia areata. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0215206"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"eaay5445","DOI":"10.1126\/scitranslmed.aay5445","article-title":"A Cutibacterium acnes antibiotic modulates human skin microbiota composition in hair follicles","volume":"12","author":"Claesen","year":"2020","journal-title":"Sci. Transl. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"293ra103","DOI":"10.1126\/scitranslmed.aab2009","article-title":"Vitamin B12 modulates the transcriptome of the skin microbiota in acne pathogenesis","volume":"7","author":"Kang","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1159\/000074111","article-title":"Is androgenetic alopecia a photoaggravated dermatosis?","volume":"207","year":"2003","journal-title":"Dermatology (Basel)"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e00793-19","DOI":"10.1128\/mSphere.00793-19","article-title":"Porphyrin production and regulation in cutaneous propionibacteria","volume":"5","author":"Barnard","year":"2020","journal-title":"mSphere"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/nrmicro.2017.157","article-title":"The human skin microbiome","volume":"16","author":"Byrd","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"229","DOI":"10.3109\/13693780902977976","article-title":"Quantitative analysis of the cutaneous Malassezia microbiota in 770 healthy Japanese by age and gender using a real-time PCR assay","volume":"48","author":"Sugita","year":"2010","journal-title":"Med. Mycol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/sj.jid.5701017","article-title":"Molecular analysis of Malassezia microflora in seborrheic dermatitis patients: Comparison with other diseases and healthy subjects","volume":"128","author":"Tajima","year":"2008","journal-title":"J. Investig. Dermatol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1111\/ced.12252","article-title":"Acne, quorum sensing and danger","volume":"39","author":"Lwin","year":"2014","journal-title":"Clin. Exp. Dermatol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/j.1087-0024.2005.10104.x","article-title":"The role of sebaceous gland activity and scalp microfloral metabolism in the etiology of seborrheic dermatitis and dandruff","volume":"10","author":"Ro","year":"2005","journal-title":"J. Investig. Dermatol. Symp. Proc."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1046\/j.1365-4362.2000.00612.x","article-title":"Androgenetic alopecia and microinflammation","volume":"39","author":"Michelet","year":"2000","journal-title":"Int. J. Dermatol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Baccaredda-Boy, A., Moretti, G., and Frey, J.R. (1968). Scalp and forehead sebum production in male patterned alopecia 1. International Symposium, Rapallo, July 1967: Proceedings. Biopathology of Pattern Alopecia, Karger.","DOI":"10.1159\/000387739"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1128\/jcm.33.4.1005-1007.1995","article-title":"Improved detection of Malassezia species in lipid-supplemented Peds Plus blood culture bottles","volume":"33","author":"Nelson","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1111\/jocd.14055","article-title":"Lipidomics demonstrates the association of sex hormones with sebum","volume":"20","author":"Chen","year":"2021","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/1523-1747.ep12260136","article-title":"Sebaceous gland response in man to prolonged total caloric deprivation","volume":"55","author":"Pochi","year":"1970","journal-title":"J. Investig. Dermatol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jdermsci.2007.11.005","article-title":"The effect of a low glycemic load diet on acne vulgaris and the fatty acid composition of skin surface triglycerides","volume":"50","author":"Smith","year":"2008","journal-title":"J. Dermatol. Sci."},{"key":"ref_49","first-page":"615","article-title":"Stress and skin: The change of physiological properties of overcrowding stress in guinea pigs","volume":"107","author":"Kaminaga","year":"1997","journal-title":"Jpn. J. Dermatol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"361","DOI":"10.3892\/etm.2017.4461","article-title":"Triptolide suppresses ultraviolet B-enhanced sebum production by inhibiting the biosynthesis of triacylglycerol in hamster sebaceous glands in vivo and in vitro","volume":"14","author":"Sato","year":"2017","journal-title":"Exp. Ther. Med."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/10\/2132\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:11:38Z","timestamp":1760166698000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/10\/2132"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,11]]},"references-count":50,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["microorganisms9102132"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms9102132","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,11]]}}}