{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:24:35Z","timestamp":1787063075984,"version":"build-2736575974"},"reference-count":59,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,31]],"date-time":"2021-10-31T00:00:00Z","timestamp":1635638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Biofilm formation in pathogenic bacteria is an important factor of resistance to antimicrobial treatments, allowing them to survive for a long time in their hosts. In the search for new antibiofilm agents, in this work we report the activity of a copper (I) complex, [Cu(NN1)2]ClO4, synthesized with Cu (I) and NN1, an imine ligand 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one, a derivate of natural compound coumarin. The antibacterial and antibiofilm capacity was evaluated in Vibrio harveyi BB170 used as model bacteria. Antibacterial activity was measured in vitro by minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and half-maximal inhibitory concentration (IC50) determination. Antibiofilm capacity of copper (I) complex was analyzed by different concentrations of IC50 values. The results showed that the sub-IC50 concentration, 12.6 \u00b5g\/mL of the copper (I) complex, was able to reduce biofilm formation by more than 75%, and bacterial viability was reduced by 50%. Inverted and confocal laser scanning microscopy showed that the [Cu(NN1)2]ClO4 complex affected the biofilm structure. Therefore, the copper (I) complex is effective as an antibiofilm compound in V. harveyi BB170.<\/jats:p>","DOI":"10.3390\/microorganisms9112273","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T22:22:24Z","timestamp":1635805344000},"page":"2273","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["In Vitro Effect of Copper (I) Complex [Cu(NN1)2](ClO4) on Vibrio harveyi BB170 Biofilm Formation"],"prefix":"10.3390","volume":"9","author":[{"given":"Sarita","family":"Soto-Aguilera","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry and Oral Biology, Faculty of Dentistry, Institute of Research in Dental Sciences, University of Chile, Olivos 943, Independencia, Santiago 8380492, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5292-0480","authenticated-orcid":false,"given":"Brenda","family":"Modak","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products Chemistry, Department of Environmental Sciences, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"},{"name":"Aquaculture Biotechnology Center, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maialen","family":"Aldabaldetrecu","sequence":"additional","affiliation":[{"name":"Laboratory of Coordination compounds and Supramolecularity, Department of Materials Chemistry, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla P.","family":"Lozano","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Oral Biology, Faculty of Dentistry, Institute of Research in Dental Sciences, University of Chile, Olivos 943, Independencia, Santiago 8380492, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9827-5162","authenticated-orcid":false,"given":"Juan","family":"Guerrero","sequence":"additional","affiliation":[{"name":"Laboratory of Coordination compounds and Supramolecularity, Department of Materials Chemistry, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Lefimil","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Oral Biology, Faculty of Dentistry, Institute of Research in Dental Sciences, University of Chile, Olivos 943, Independencia, Santiago 8380492, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mick","family":"Parra","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products Chemistry, Department of Environmental Sciences, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"},{"name":"Aquaculture Biotechnology Center, Faculty of Chemistry and Biology, University of Santiago of Chile, Av. Bernardo O\u2019Higgins 3363, Santiago 9170022, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1007\/s12275-017-7274-x","article-title":"Antibiofilm Agents: A New Perspective for Antimicrobial Strategy","volume":"55","author":"Li","year":"2017","journal-title":"J. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mulcahy, H., Charron-Mazenod, L., and Lewenza, S. (2008). Extracellular DNA Chelates Cations and Induces Antibiotic Resistance in Pseudomonas Aeruginosa Biofilms. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000213"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"493","DOI":"10.4155\/fmc.15.6","article-title":"Biofilm Formation Mechanisms and Targets for Developing Antibiofilm Agents","volume":"7","author":"Rabin","year":"2015","journal-title":"Future Med. 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