{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:31:28Z","timestamp":1787941888323,"version":"build-2784847793"},"reference-count":37,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T00:00:00Z","timestamp":1639699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Erysipelothrix rhusiopathiae can cause erysipelas in animals and erysipeloid in humans. Since its recurrence in 2012, swine erysipelas has caused serious losses within the pig industry in China. The aim of this study was to perform multilocus sequence typing and understand the virulence and antimicrobial susceptibility of E. rhusiopathiae isolates in China. Multilocus sequence typing (MLST) of a total of 120 strains was performed, and as a result, three different sequence types were identified, of which ST48 was the main one. Five isolates of each MLST type were randomly selected to be used to challenge mice. ST48 was associated with a higher virulence. Antimicrobial susceptibility was tested using a microdilution technique and, to analyze the resistance mechanism, six strains were selected for genome sequencing. A comparison of the six genomes indicated the presence of a suspected macrolide resistance gene, namely, Erm(A)-like, in erythromycin-resistant strains, which increased the minimum inhibitory concentration (MIC) of erythromycin against E. coli C600 at least four-fold. In addition, three mutations (gyrA86T-I, gyrA90D-N, and parC81S-I) were observed in the quinolone resistance-determining regions (QRDRs) of gyrA and parC in quinolone-resistant strains. After the gyrA gene with the 86T-I mutation or the parC gene with the 81S-I mutation was transfected into E. coli C600, the MIC of enrofloxacin against this strain increased at least two-fold. Our findings provide a theoretical basis for developing antibacterial drugs and may contribute to the clinical prevention and control of E. rhusiopathiae.<\/jats:p>","DOI":"10.3390\/microorganisms9122615","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T02:50:18Z","timestamp":1639968618000},"page":"2615","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Characterization of Erysipelothrix rhusiopathiae Isolates from Diseased Pigs in 15 Chinese Provinces from 2012 to 2018"],"prefix":"10.3390","volume":"9","author":[{"given":"Chao","family":"Wu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjie","family":"Lv","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weifeng","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Kang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomei","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6503-8590","authenticated-orcid":false,"given":"Qiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biomedicine and Health, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4332-4083","authenticated-orcid":false,"given":"Meilin","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1111\/j.1365-2672.2009.04560.x","article-title":"Rapid detection and differentiation of Erysipelothrix spp. by a novel multiplex real-time PCR assay","volume":"108","author":"Pal","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1186\/s13567-015-0216-x","article-title":"A combinational approach of multilocus sequence typing and other molecular typing methods in unravelling the epidemiology of Erysipelothrix rhusiopathiae strains from poultry and mammals","volume":"46","author":"Janssen","year":"2015","journal-title":"Vet. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1080\/03079457.2010.518313","article-title":"Characterization of Erysipelothrix rhusiopathiae isolates from laying hens and poultry red mites (Dermanyssus gallinae) from an outbreak of erysipelas","volume":"39","author":"Eriksson","year":"2010","journal-title":"Avian Pathol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1177\/0300985814556187","article-title":"Acute and Chronic Erysipelothrix rhusiopathiae Infection in Lambs","volume":"52","author":"Ersdal","year":"2015","journal-title":"Vet. Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Jaeger, A., Lebarbenchon, C., Bourret, V., Bastien, M., Lagadec, E., Thiebot, J.B., Boulinier, T., Delord, K., Barbraud, C., and Marteau, C. (2018). Avian cholera outbreaks threaten seabird species on Amsterdam Island. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0197291"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1292\/jvms.10-0248","article-title":"Characterization and identification of Erysipelothrix rhusiopathiae isolated from an unnatural host, a cat, with a clinical manifestation of depression","volume":"73","author":"Lee","year":"2011","journal-title":"J. Vet. Med. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"434-e134","DOI":"10.1111\/vde.12773","article-title":"Erysipeloid lesions caused by Erysipelothrix rhusiopathiae in a dog: Clinical and histopathological findings, molecular diagnosis and treatment","volume":"30","author":"Marshall","year":"2019","journal-title":"Vet. Dermatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1111\/1348-0421.12736","article-title":"Wild boars: A potential source of Erysipelothrix rhusiopathiae infection in Japan","volume":"63","author":"Shimoji","year":"2019","journal-title":"Microbiol. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Cabrera-Garcia, A.I., Muller, F., Rodler, F.S., Traub, F., and Heilmann, R.M. (2020). Infective endocarditis due to Erysipelothrix rhusiopathiae in a dog\u2014A case report. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02546-6"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.vetmic.2014.06.017","article-title":"Long-term monitoring of 10 selected pathogens in wild boar (Sus scrofa) in Sierra Nevada National Park, southern Spain","volume":"174","author":"Fandos","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.tvjl.2017.04.012","article-title":"Serotypes and Spa types of Erysipelothrix rhusiopathiae isolates from British pigs (1987 to 2015)","volume":"225","author":"McNeil","year":"2017","journal-title":"Vet. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1292\/jvms.11-0533","article-title":"Characterization of Erysipelothrix rhusiopathiae strains isolated from recent swine erysipelas outbreaks in Japan","volume":"74","author":"To","year":"2012","journal-title":"J. Vet. Med. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e69","DOI":"10.1038\/emi.2015.69","article-title":"Virulence determinants, antimicrobial susceptibility, and molecular profiles of Erysipelothrix rhusiopathiae strains isolated from China","volume":"4","author":"Ding","year":"2015","journal-title":"Emerg. Microbes Infect."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Kwok, A.H., Li, Y., Jiang, J., Jiang, P., and Leung, F.C. (2014). Complete genome assembly and characterization of an outbreak strain of the causative agent of swine erysipelas\u2014Erysipelothrix rhusiopathiae SY1027. BMC Microbiol., 14.","DOI":"10.1186\/1471-2180-14-176"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Yang, L., Zhu, Y., Peng, Z., Ding, Y., Jie, K., Wang, Z., Peng, Y., Tang, X., Wang, X., and Chen, H. (2020). Comparative Genome Analysis of a Pathogenic Erysipelothrix rhusiopathiae Isolate WH13013 from Pig Reveals Potential Genes Involve in Bacterial Adaptions and Pathogenesis. Vet. Sci., 7.","DOI":"10.3390\/vetsci7020074"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1292\/jvms.09-0448","article-title":"Antimicrobial susceptibility of Erysipelothrix rhusiopathiae isolated from pigs in Southern Japan with a modified agar dilution method","volume":"72","author":"Chuma","year":"2010","journal-title":"J. Vet. Med. Sci."},{"key":"ref_17","first-page":"115","article-title":"Antimicrobial susceptibilities of Erysipelothrix rhusiopathiae isolated from pigs with swine erysipelas in Japan, 1988\u20131998","volume":"48","author":"Yamamoto","year":"2001","journal-title":"J. Vet. Med. B Infect. Dis. Vet. Public Health"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"9908","DOI":"10.1038\/s41598-019-45482-8","article-title":"Prevalence and antimicrobial susceptibilities of bacterial pathogens in Chinese pig farms from 2013 to 2017","volume":"9","author":"Zhang","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1046\/j.1439-0450.2001.00442.x","article-title":"Identification of the tetracycline resistance gene, tet(M), in Erysipelothrix rhusiopathiae","volume":"48","author":"Yamamoto","year":"2001","journal-title":"J. Vet. Med. B Infect. Dis. Vet. Public Health"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.vetmic.2015.02.014","article-title":"Presence and new genetic environment of pleuromutilin-lincosamide-streptogramin A resistance gene lsa(E) in Erysipelothrix rhusiopathiae of swine origin","volume":"177","author":"Zhang","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2462","DOI":"10.1128\/AAC.00228-15","article-title":"First report of macrolide resistance gene erm(T) harbored by a novel small plasmid from Erysipelothrix rhusiopathiae","volume":"59","author":"Xu","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jprot.2017.03.004","article-title":"iTRAQ-based quantitative proteomic analysis reveals potential virulence factors of Erysipelothrix rhusiopathiae","volume":"160","author":"Wang","year":"2017","journal-title":"J. Proteom."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1093\/femsle\/fnx036","article-title":"Erysipelothrix rhusiopathiae recruits host plasminogen via the major protective antigen SpaA","volume":"364","author":"Zhu","year":"2017","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"108649","DOI":"10.1016\/j.vetmic.2020.108649","article-title":"Characterization of Pasteurella multocida isolates from rabbits in China","volume":"244","author":"Zhu","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5957","DOI":"10.2147\/CMAR.S313775","article-title":"LncRNA IGFL2-AS1 Promotes the Proliferation, Migration, and Invasion of Colon Cancer Cells and is Associated with Patient Prognosis","volume":"13","author":"Cen","year":"2021","journal-title":"Cancer Manag. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/nmeth.2474","article-title":"Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data","volume":"10","author":"Chin","year":"2013","journal-title":"Nat. Methods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1101\/gr.215087.116","article-title":"Canu: Scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation","volume":"27","author":"Koren","year":"2017","journal-title":"Genome Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Chaisson, M.J., and Tesler, G. (2012). Mapping single molecule sequencing reads using basic local alignment with successive refinement (BLASR): Application and theory. BMC Bioinform., 13.","DOI":"10.1186\/1471-2105-13-238"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1093\/bioinformatics\/btm009","article-title":"Identifying bacterial genes and endosymbiont DNA with Glimmer","volume":"23","author":"Delcher","year":"2007","journal-title":"Bioinformatics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3422","DOI":"10.1093\/bioinformatics\/bti553","article-title":"ACT: The Artemis Comparison Tool","volume":"21","author":"Carver","year":"2005","journal-title":"Bioinformatics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1128\/jcm.32.6.1526-1531.1994","article-title":"Direct and rapid detection of Erysipelothrix rhusiopathiae DNA in animals by PCR","volume":"32","author":"Makino","year":"1994","journal-title":"J. Clin. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8079","DOI":"10.1016\/j.vaccine.2018.10.071","article-title":"Evaluation of the protective efficacy of four newly identified surface proteins of Erysipelothrix rhusiopathiae","volume":"36","author":"Zhu","year":"2018","journal-title":"Vaccine"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e00377-19","DOI":"10.1128\/JCM.00377-19","article-title":"Serotype and Genotype (Multilocus Sequence Type) of Streptococcus suis Isolates from the United States Serve as Predictors of Pathotype","volume":"57","author":"Estrada","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.vetmic.2013.06.009","article-title":"Characterization of Streptococcus suis serotype 2 isolates from China","volume":"166","author":"Zhu","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1007\/s44197-021-00008-2","article-title":"Easy Access to Antibiotics; Spread of Antimicrobial Resistance and Implementation of One Health Approach in India","volume":"11","author":"Jani","year":"2021","journal-title":"J. Epidemiol. Glob. Health"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"105306","DOI":"10.1016\/j.micpath.2021.105306","article-title":"Research on the drug resistance mechanism of foodborne pathogens","volume":"162","author":"Ge","year":"2022","journal-title":"Microb. Pathog."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1128\/MMBR.64.4.672-693.2000","article-title":"Molecular properties of bacterial multidrug transporters","volume":"64","author":"Putman","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev. MMBR"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/12\/2615\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:50:58Z","timestamp":1760169058000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/9\/12\/2615"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,17]]},"references-count":37,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["microorganisms9122615"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms9122615","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,17]]}}}