{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T09:25:36Z","timestamp":1780478736798,"version":"3.54.1"},"reference-count":12,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2008,3,4]],"date-time":"2008-03-04T00:00:00Z","timestamp":1204588800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>We report here the characterisation of eight newly synthesized thioureides of 2-(4-chlorophenoxymethyl)-benzoic acid and the evaluation of the in vitro antimicrobialactivity of the new compounds against Gram-positive [Listeria monocytogenes,Staphylococcus aureus, Bacillus subtilis], Gram-negative [Psedomonas aeruginosa,Escherichia coli, Salmonella enteritidis], as well as Candida spp., using both reference andclinical multidrug resistant strains to establish the minimal inhibitory concentration (MIC)values. Our results showed that the tested compounds exhibited specific antimicrobialactivities, both concerning the spectrum of antimicrobial activity and the correspondingMIC values, which ranged widely between 1024 and 32 \u03bcg\/mL, depending on the natureand position of the substituents on the benzene ring. The most active compounds were N-[2-(4-chlorophenoxymethyl)-benzoyl]-N'-(2,6-dichlorophenyl)-thiourea (5g) and N-[2-(4-chlorophenoxymethyl)-benzoyl]-N'-(4-bromophenyl)-thiourea (5h), which showed a broadspectrum of antimicrobial activity against enterobacterial strains (E. coli and S. enteritidis),P. aeruginosa, S. aureus and Candida spp. All the tested compounds except 5f were highly active against S. aureus (MIC=32 \u03bcg\/mL), suggesting their possible use in the treatment ofMRSA infections. Four of compounds also exhibited antifungal activity (MIC =256-32\u03bcg\/mL) against C. albicans, but L. monocytogenes as well as B. subtilis were resistant toall tested compounds. Our studies thus demonstrated that among other biological activities,the thioureides of 2-(4-chlorophenoxymethyl)-benzoic acid also exhibit selective andeffective antimicrobial properties that could lead to the selection and use of thesecompounds as efficient antimicrobial agents, especially for the treatment of multidrugresistant infections.<\/jats:p>","DOI":"10.3390\/molecules13030567","type":"journal-article","created":{"date-parts":[[2008,10,2]],"date-time":"2008-10-02T12:45:09Z","timestamp":1222951509000},"page":"567-580","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Antimicrobial Activity of Some New Thioureides Derived from 2-(4-Chlorophenoxymethyl)benzoic Acid"],"prefix":"10.3390","volume":"13","author":[{"given":"Carmen","family":"Limban","sequence":"first","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Farmacy, \u201cCarol Davila\u201d University of Medicine and Pharmacy, Traian Vuia 6, sect. 2, 020956, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana-Carmen Balotescu","family":"Chifiriuc","sequence":"additional","affiliation":[{"name":"Microbiology-Immunology Department, Faculty of Biology, University of Bucharest, Ale. Portocalelor 1-3, sect. 5, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandru-Vasile","family":"Missir","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Farmacy, \u201cCarol Davila\u201d University of Medicine and Pharmacy, Traian Vuia 6, sect. 2, 020956, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ileana Cornelia","family":"Chiri\u0163\u0103","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Farmacy, \u201cCarol Davila\u201d University of Medicine and Pharmacy, Traian Vuia 6, sect. 2, 020956, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9031-338X","authenticated-orcid":false,"given":"Coralia","family":"Bleotu","sequence":"additional","affiliation":[{"name":"Institute of Virology \u201cProf. St. Nicolau\u201d, Sos. M. Bravu, 36, sect.2, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2008,3,4]]},"reference":[{"key":"ref_1","first-page":"73","article-title":"Noi tioureide ale acidului 2-fenoximetil-benzoic. Nota I","volume":"6","author":"Limban","year":"2000","journal-title":"Farmacia"},{"key":"ref_2","first-page":"7","article-title":"Noi tioureide ale acidului 2-fenoximetil-benzoic. Nota II","volume":"5","author":"Limban","year":"2004","journal-title":"Farmacia"},{"key":"ref_3","unstructured":"Limban, C., Missir, Al., Chiri\u0163\u0103, I., and Missir, C. (2005, January 22-24). The synthesis and characterization of some new thioureides of 2-(4-methylphenoxymethyl)-benzoic acid with potential pharmacological activity. Romanian International Conference on Chemistry and Chemical Engineering-RICCCE XIV, Bucarest, Rumania. S-02-99."},{"key":"ref_4","first-page":"42","article-title":"Synthesis and characterization of some new thioureides of 2-(4-methoxyphenoxymethyl)-benzoic acid with potential pharmacological activity","volume":"55","author":"Limban","year":"2005","journal-title":"TimisoaraMed. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1582-4934.2001.tb00137.x","article-title":"Detection of human papillomavirus gene sequence in cell lines derived from laryngeal tumors","volume":"5","author":"Mutiu","year":"2001","journal-title":"J. Cell. Mol. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1111\/j.1582-4934.2004.tb00263.x","article-title":"The development of larger cells that spontaneously escape senescence - a step during the immortalization of a human cancer cell line","volume":"8","author":"Diaconu","year":"2004","journal-title":"J. Cell. Mol. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/s10973-005-0676-8","article-title":"Thermal behavior, spectroscopic and biological characterization of Co (II), Zn (II) and Pt (II) complexes with N,N-dimethylbiguanide","volume":"80","author":"Olar","year":"2005","journal-title":"J. Therm. Anal. Calorim."},{"key":"ref_8","first-page":"2225","article-title":"Comparative evaluation of qualitative and quantitative methods used in the study of antifungal and antibacterial activity of hydroalcoholic vegetal extracts","volume":"10","author":"Lazar","year":"2005","journal-title":"Roum. Biotechnol. Lett."},{"key":"ref_9","first-page":"2481","article-title":"Antibacterial, antifungal and cytotoxic activity of salvia officinalis essential oil and tinctures","volume":"10","author":"Balotescu","year":"2005","journal-title":"Roum. Biotechnol. Lett."},{"key":"ref_10","unstructured":"Clinical and Laboratory Standards Institute (2007). Performance Standards for Antimicrobial Susceptibility Testing, CLSI. Seventeenth Informational Supplement, M100-S17, Vol. 27 No. 1, Replaces M100-S16, Vol. 26 No. 3, Informational Supplement."},{"key":"ref_11","unstructured":"Clinical and Laboratory Standards Institute (1999). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, CLSI. Approved standard M7-A2."},{"key":"ref_12","first-page":"1064","article-title":"The Synthesis and Biological Activities Of Some New 2-(4-Methoxyphenoxymethyl)benzoic Acid Thioureides","volume":"58","author":"Balotescu","year":"2007","journal-title":"Rev. Chim. (Bucuresti)"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/13\/3\/567\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T22:20:29Z","timestamp":1760221229000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/13\/3\/567"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3,4]]},"references-count":12,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2008,3]]}},"alternative-id":["13030567"],"URL":"https:\/\/doi.org\/10.3390\/molecules13030567","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3,4]]}}}