{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T18:47:20Z","timestamp":1777747640184,"version":"3.51.4"},"reference-count":30,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Aqueous extracts from seeds of Areca catechu L. (Arecaceae) (AC) and nutgalls of Quercus infectoria Oliv. (Fagaceae) (QI) were investigated for their hepatoprotective potential by studying their antioxidant capacity using four different methods, by determining their in vitro anti\u2212inflammatory activity against 5-lipoxygenase, and by evaluating their hepatoprotective potential against liver injury induced by carbon tetrachloride (CCl4) in rats. AC and QI extracts exhibited potent antioxidant and anti-inflammatory activities. Treatment of rats with AC and QI extracts reversed oxidative damage in hepatic tissues induced by CCl4. It is suggested that extracts rich in either condensed or hydrolysable tannins and known for their potent antioxidant and anti-inflammatory activities, may potentially confer protection against oxidative stress\u2212induced liver injury. These data should contribute to evidence-based traditional medicines for anti-inflammatory and hepatoprotective effects of both extracts.<\/jats:p>","DOI":"10.3390\/molecules14124987","type":"journal-article","created":{"date-parts":[[2009,12,2]],"date-time":"2009-12-02T03:06:01Z","timestamp":1259723161000},"page":"4987-5000","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Hepatoprotective Potential of Extracts from Seeds of Areca catechu and Nutgalls of Quercus infectoria"],"prefix":"10.3390","volume":"14","author":[{"given":"Pimolpan","family":"Pithayanukul","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saruth","family":"Nithitanakool","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rapepol","family":"Bavovada","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10300, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2009,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1093\/oxfordjournals.bmb.a072634","article-title":"Liver damage due to free radicals","volume":"49","author":"Poli","year":"1993","journal-title":"Br. Med. Bull."},{"key":"ref_2","first-page":"307","article-title":"Natural products and plants as liver protecting drugs","volume":"57","author":"Handa","year":"1986","journal-title":"Fitoterapia"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0300-483X(00)00210-9","article-title":"Free radicals and grape seed proanthocyanidin extract: Importance in human health and disease prevention","volume":"148","author":"Bagchi","year":"2000","journal-title":"Toxicology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4444","DOI":"10.1021\/jf8002174","article-title":"Walnut polyphenols prevent liver damage induced by carbontetrachloride and D-galactosamine: Hepatoprotective hydrolysable tannins in the kernel pellicles of walnut","volume":"56","author":"Shimoda","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0304-3835(95)03727-E","article-title":"Modulatory influence of arecanut on antioxidant 2(3)-tert-butyl-4-hydroxy anisole-induced hepatic detoxification system and antioxidant defense mechanism in mice","volume":"91","author":"Anjali","year":"1995","journal-title":"Cancer Lett."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0378-8741(96)01503-6","article-title":"Evaluation of four prescriptions of traditional Chinese medicine: Syh-mo-yiin, guizhi-fuling-wan, shieh-qing-wan and syh-nih-sann on experimental acute liver damage in rats","volume":"55","author":"Tsai","year":"1997","journal-title":"J. Ethnopharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1039\/c39810000781","article-title":"Structures of dimeric, trimeric, and tetrameric procyanidins from Areca catechu L","volume":"32","author":"Nonaka","year":"1981","journal-title":"J. Chem. Soc. Chem. Commun."},{"key":"ref_8","first-page":"255","article-title":"Glossary of indian medicinal plant","volume":"208","author":"Chopra","year":"1956","journal-title":"C. S.I. R. New Delhi India"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.cbi.2007.10.002","article-title":"Quercus infectoria galls possess antioxidant activity and abrogate oxidative stress-induced functional alterations in murine macrophages","volume":"171","author":"Kaur","year":"2008","journal-title":"Chem. Biol. Interact."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1080\/10408690490468489","article-title":"Antioxidants and prevention of chronic disease","volume":"44","author":"Willcox","year":"2004","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1046\/j.1467-2494.1999.196590.x","article-title":"The effects of Areca catechu L. extract on anti-inflammation and anti-melanogenesis","volume":"21","author":"Lee","year":"1999","journal-title":"Int. J. Cosmet. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.jep.2003.10.009","article-title":"Antiinflammatory evaluation of alcoholic extract of galls of Quercus infectoria","volume":"90","author":"Kaur","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1159\/000139114","article-title":"Effects of leukotriene synthesis inhibition on acute liver damage induced by CCl4","volume":"47","author":"Muriel","year":"1993","journal-title":"Pharmacology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/0163-7258(89)90050-8","article-title":"Mechanisms of carbon tetrachloride toxicity","volume":"43","author":"Recknagel","year":"1989","journal-title":"Pharmacol. Ther."},{"key":"ref_15","first-page":"9","article-title":"Carbon tetrachloride hepatotoxicity: An example of lethal cleavage","volume":"2","author":"Recknagel","year":"1973","journal-title":"CRC Crit. Rev. Toxicol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s12263-007-0027-4","article-title":"Grape seed procyanidins inhibit the expression of metallothione in genes in human HepG2 cells","volume":"2","author":"Quesada","year":"2007","journal-title":"Genes. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4357","DOI":"10.1021\/jf0633185","article-title":"Grape-seed procyanidins act as anti-inflammatory agents in endotoxin-stimulated RAW 264.7 macrophages by inhibiting NFkB signaling pathway","volume":"55","author":"Terra","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.abb.2005.02.016","article-title":"The antioxidant effect of tannic acid on the in vitro copper-mediated formation of free radicals","volume":"437","author":"Andrade","year":"2005","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1615\/JEnvironPatholToxicolOncol.v26.i1.20","article-title":"Abrogation of thioacetamide-induced biochemical events of hepatic tumor promotion stage by tannic acid in Wistar rats","volume":"26","author":"Sehrawat","year":"2007","journal-title":"J. Environ. Pathol. Toxicol. Oncol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.toxlet.2007.12.012","article-title":"Tannic acid prevents azidothymidine (AZT) induced hepatotoxicity and genotoxicity along with change in expression of PARG and histone H3 acetylation","volume":"177","author":"Tikoo","year":"2008","journal-title":"Toxicol. Lett."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.jep.2004.12.013","article-title":"Inhibition of Naja kaouthia venom activities by plant polyphenols","volume":"97","author":"Pithayanukul","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S0076-6879(99)99017-1","article-title":"Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent","volume":"299","author":"Singleton","year":"1999","journal-title":"Meth. Enzymol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0031-9422(00)94533-3","article-title":"The conversion of procyanidins and prodelphinidins to cyaniding and delphinidin","volume":"25","author":"Porter","year":"1986","journal-title":"Phytochemistry"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s11094-006-0063-1","article-title":"Quantitative determination of procyanidins in hawthorn fruits","volume":"40","author":"Khishova","year":"2006","journal-title":"Pharm. Chem. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1111\/j.1365-2621.1993.tb06194.x","article-title":"Antioxidant effects of some ginger constituents","volume":"58","author":"Kikuzaki","year":"1993","journal-title":"J. Food Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0023-6438(95)80008-5","article-title":"Use of a free radical method to evaluate antioxidant activity","volume":"28","author":"Cuvelier","year":"1995","journal-title":"Lebensm. Wiss. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0003-2697(87)90222-3","article-title":"The deoxyribose method: A simple \"test\u2212tube\" assay for determination of rate constants for reactions of hydroxyl radicals","volume":"165","author":"Halliwell","year":"1987","journal-title":"Anal. Biochem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1002\/ptr.2069","article-title":"Determination of antioxidant and radical scavenging activity of Basil (Ocimum basilicum L. Family Lamiaceae) assayed by different methodologies","volume":"21","year":"2007","journal-title":"Phytother. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/S0962-4562(03)00083-3","article-title":"Inhibition of 5-lipoxygenase by essential oils and other natural fragrant extracts","volume":"13","author":"Baylac","year":"2003","journal-title":"Intern. J. Aromather."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Anal. Biochem."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/14\/12\/4987\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T22:11:51Z","timestamp":1760220711000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/14\/12\/4987"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12,1]]},"references-count":30,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2009,12]]}},"alternative-id":["14124987"],"URL":"https:\/\/doi.org\/10.3390\/molecules14124987","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12,1]]}}}