{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T22:51:45Z","timestamp":1787957505722,"version":"build-2784847793"},"reference-count":35,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2010,5,14]],"date-time":"2010-05-14T00:00:00Z","timestamp":1273795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Aromatase represents an important target for the treatment of hormone-dependent breast cancer. In the present study, nine alkaloids from the dichloromethane extract of Corydalis yanhusuo were identified by liquid chromatography tandem mass spectrometry (LC-MS\/MS) and tested for their aromatase binding activities using an ultrafiltration LC-MS method by investigating the differences of peak areas of compounds before and after incubations with aromatase. It was demonstrated that the quaternary protoberberine alkaloids and the tertiary protoberberine alkaloids exhibited potent aromatase binding activities. The quaternary ammonium group and the methyl group at C-13 position of tertiary protoberberine alkaloids might be necessary for the activity. The findings should provide guidance for the discovery of potential aromatase inhibitors from natural products.<\/jats:p>","DOI":"10.3390\/molecules15053556","type":"journal-article","created":{"date-parts":[[2010,5,13]],"date-time":"2010-05-13T13:10:57Z","timestamp":1273756257000},"page":"3556-3566","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Characterization of Aromatase Binding Agents from the Dichloromethane Extract of Corydalis yanhusuo Using Ultrafiltration and Liquid Chromatography Tandem Mass Spectrometry"],"prefix":"10.3390","volume":"15","author":[{"given":"Jing","family":"Shi","sequence":"first","affiliation":[{"name":"1-Department of Pharmacy, Zhejiang Medical College, No.481 Binwen Rd., Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"2-School of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, No.388 Yuhangtang Rd., Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongjun","family":"Ma","sequence":"additional","affiliation":[{"name":"2-School of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, No.388 Yuhangtang Rd., Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"2-School of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, No.388 Yuhangtang Rd., Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Sun","sequence":"additional","affiliation":[{"name":"2-School of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, No.388 Yuhangtang Rd., Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2010,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1124\/pr.57.3.6","article-title":"Regulation of aromatase expression in estrogen-responsive breast and uterine disease: from bench to treatment","volume":"57","author":"Bulun","year":"2005","journal-title":"Pharmacol. 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