{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:38:24Z","timestamp":1760243904828,"version":"build-2065373602"},"reference-count":23,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The dermatophytes are filamentous fungi that cause cutaneous fungal infections because they use keratin as a nutrient source. For this study the antidermatophyte activity of the extracts and derivates from leaves of Piper regnellii was analyzed. From the dichloromethane extract (EBD) neolignans such as eupomatenoid-3 and eupomatenoid-5 were obtained, and it was submitted to fractionation to remove the green residue, designated as the chloroform fraction (FF). Extracts, chloroform fraction and compounds were tested against Trichophyton rubrum ATCC 28189 to determine the minimum inhibitory concentration (MIC). The chloroform fraction was incorporated to nail lacquer that was analyzed by photoacoustic spectroscopy, in vitro assay and scanning electronic microscopy. For antifungal activity in solid medium the dichloromethane extract and chloroform fraction were used. The compounds eupomatenoid-3 and eupomatenoid-5 were less active than the dichloromethane extract against T. rubrum. EBD and FF showed moderate activity in hyphal growth inhibition in solid medium and EBD did not link to ergosterol. Nail lacquer containing the chloroform fraction showed good penetration through the nail as determined by photoacoustic spectroscopy. From in vitro studies it was observed that nail lacquer concentrations above 20 mg\/mL prevented the growth of fungi, but concentrations up to 2.5 inhibited the growth. Scanning electronic microscopy was used to confirm the in vitro nail lacquer activity results. The specie P. regnellii showed great antifungal activity against T. rubrum, and nail lacquer containing its chloroform fraction has great potential to treat onychomycosis caused by these microorganisms.<\/jats:p>","DOI":"10.3390\/molecules15063920","type":"journal-article","created":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T13:01:34Z","timestamp":1275397294000},"page":"3920-3931","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Antifungal Activity and Nail Permeation of Nail Lacquer Containing Piper regnellii (Miq.) C. CD. var. pallescens (C. DC.) Yunck (Piperaceae) Leave Extracts and Derivatives"],"prefix":"10.3390","volume":"15","author":[{"given":"Andrea Mayumi","family":"Koroishi","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Estadual de Maring\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizandra","family":"Sehn","sequence":"additional","affiliation":[{"name":"P\u00f3s-gradua\u00e7\u00e3o em F\u00edsica, Departamento de F\u00edsica, Universidade Estadual de Maring\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro Luciano","family":"Baesso","sequence":"additional","affiliation":[{"name":"P\u00f3s-gradua\u00e7\u00e3o em F\u00edsica, Departamento de F\u00edsica, Universidade Estadual de Maring\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e2nia","family":"Ueda-Nakamura","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Estadual de Maring\u00e1, Brazil"},{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Estadual de Maring\u00e1, Av. Colombo, 5790, 87020-900, Maring\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celso Vataru","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Estadual de Maring\u00e1, Brazil"},{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Estadual de Maring\u00e1, Av. Colombo, 5790, 87020-900, Maring\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di\u00f3genes Apar\u00edcio Garcia","family":"Cortez","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Estadual de Maring\u00e1, Brazil"},{"name":"Departamento de Farm\u00e1cia e Farmacologia, Universidade Estadual de Maring\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedito Prado Dias","family":"Filho","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Estadual de Maring\u00e1, Brazil"},{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Estadual de Maring\u00e1, Av. Colombo, 5790, 87020-900, Maring\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2010,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1128\/CMR.8.2.240","article-title":"The dermatophytes","volume":"8","author":"Weitzman","year":"1995","journal-title":"Clin. Microb. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.jep.2005.02.011","article-title":"Ethnoveterinary medicine in the search for antimicrobial agents: Antifungal activity of some species of Pterocaulon (Asteraceae)","volume":"99","author":"Stein","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/j.bmc.2006.09.038","article-title":"Synthesis and antifungal activity of (Z)-5-aryldenerhodanines","volume":"15","author":"Sortino","year":"2007","journal-title":"Bioorg. Med. 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IV"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/15\/6\/3920\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T22:02:34Z","timestamp":1760220154000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/15\/6\/3920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,1]]},"references-count":23,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2010,6]]}},"alternative-id":["15063920"],"URL":"https:\/\/doi.org\/10.3390\/molecules15063920","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2010,6,1]]}}}