{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T19:38:19Z","timestamp":1781638699493,"version":"3.54.5"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2010,8,30]],"date-time":"2010-08-30T00:00:00Z","timestamp":1283126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Extracts from Dipteryx alata bark obtained with different solvents (hexane, dichloromethane, ethyl acetate and methanol) were mixed in vitro with Bothrops jararacussu (Bjssu, 40 \u03bcg\/mL) and Crotalus durissus terrificus (Cdt, 15 \u03bcg\/mL) snake venoms, and applied to a mouse phrenic nerve-diaphragm preparation to evaluate the possible neutralization of venom effects. Cdt venom neurotoxic effect was not inhibited by any of the extracts, while the neurotoxic and myotoxic actions of Bjssu venom were decreased by the methanolic extract. This inhibition appears to be augmented by tannins. Dichloromethane bark extract inhibited ~40% of Bjssu venom effects and delayed blockade induced by Cdt. The methodology used to determine which extract was active allows inferring that: (i) phenolic acids and flavonoids contained in the methanolic extract plus tannins were responsible mostly for neutralization of Bjssu effects; (ii) terpenoids from the dichloromethane extract may participate in the anti-Cdt and anti-Bjssu venom effects; (iii) a given extract could not inhibit venoms from different species even if those belong to the same family, so it is improper to generalize a certain plant as antiophidian; (iv) different polarity extracts do not present the same inhibitory capability, thus demonstrating the need for characterizing both venom pharmacology and the phytochemistry of medicinal plant compounds<\/jats:p>","DOI":"10.3390\/molecules15095956","type":"journal-article","created":{"date-parts":[[2010,8,30]],"date-time":"2010-08-30T10:45:18Z","timestamp":1283165118000},"page":"5956-5970","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["In Vitro Antiophidian Properties of Dipteryx alata Vogel Bark Extracts"],"prefix":"10.3390","volume":"15","author":[{"given":"Virg\u00ednia Sbrugnera","family":"Nazato","sequence":"first","affiliation":[{"name":"Universidade de Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, Zip code 18023-000, Sorocaba, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro","family":"Rubem-Mauro","sequence":"additional","affiliation":[{"name":"Universidade de Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, Zip code 18023-000, Sorocaba, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalia Aparecida Gatto","family":"Vieira","sequence":"additional","affiliation":[{"name":"Universidade de Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, Zip code 18023-000, Sorocaba, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"J\u00fanior","given":"Dimas","family":"Dos Santos Rocha","sequence":"additional","affiliation":[{"name":"Universidade de Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, Zip code 18023-000, Sorocaba, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Glauzer Silva","sequence":"additional","affiliation":[{"name":"Universidade de Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, Zip code 18023-000, Sorocaba, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Santos Lopes","sequence":"additional","affiliation":[{"name":"Universidade de Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, Zip code 18023-000, Sorocaba, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ch\u00e1riston Andr\u00e9","family":"Dal-Belo","sequence":"additional","affiliation":[{"name":"Universidade Federal do Pampa, UNIPAMPA, Av. Ant\u00f4nio Trilha, 1847, Zip code 97300-000, S\u00e3o Gabriel, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Carlos","family":"Cogo","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Paraiba, UNIVAP, Av. Shishima Hifumi, 2911- Urbanova, Zip code 12244-000, S\u00e3o Jos\u00e9 dos Campos, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcio Galdino","family":"Dos Santos","sequence":"additional","affiliation":[{"name":"Universidade Federal do Tocantins, UFT, Av. NS 15 ALC NO 14, 109 Norte, Zip code 77001-090, Porto Nacional, TO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Alice","family":"Da Cruz-H\u00f6fling","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas, UNICAMP, R. Monteiro Lobato, 255, Zip code 13083-862, Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Oshima-Franco","sequence":"additional","affiliation":[{"name":"Universidade de Sorocaba, UNISO, Rodovia Raposo Tavares, km 92.5, Zip code 18023-000, Sorocaba, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2010,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.neuropharm.2007.05.002","article-title":"Venom therapy in multiple sclerosis","volume":"53","author":"Mirshafiey","year":"2007","journal-title":"Neuropharmacology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1016\/S0041-0101(01)00169-6","article-title":"Neutralization of proteases from Bothrops snake venoms by the aqueous extract from Casearia sylvestris (Flacourtiaceae)","volume":"39","author":"Borges","year":"2001","journal-title":"Toxicon"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jep.2005.01.063","article-title":"Antihemorrhagic, antinucleolytic and other antiophidian properties of the aqueous extract from Pentaclethra macroloba","volume":"100","author":"Coppede","year":"2005","journal-title":"J. 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