{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:37:17Z","timestamp":1789706237708,"version":"4.0.1"},"reference-count":27,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2010,9,9]],"date-time":"2010-09-09T00:00:00Z","timestamp":1283990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Molecules"],"abstract":"<jats:p>Samples of unifloral sulla (Hedysarum coronarum L.) honey from Sardinia (Italy) were analysed. To investigate the chemical composition of the honey volatiles two solvent systems were used for ultrasonic solvent extraction (USE): 1) a 1:2 (v\/v) pentane and diethyl ether mixture and 2) dichloromethane. All the extracts were analysed by GC and GC\/MS. These procedures have permitted the identification of 56 compounds that include norisoprenoids, benzene derivatives, aliphatic compounds and Maillard reaction products. Norisoprenoids were the major compounds in both extracts, dominated by vomifoliol (5.3-11.2%; 9.6-14.0%) followed by minor percentages of other norisoprenoids such as \u03b1-isophorone, 4-ketoisophorone, 3-oxo-\u03b1-ionol or 3-oxo-\u03b1-ionone. Other abundant single compounds in the extracts were 3-hydroxy-4-phenylbutan-2-one (0.8-5.4%; 0.6-5.7%) and methyl syringate (3.0-5.7%; 2.2-4.1%). The composition of the volatiles and semi-volatiles in the obtained extracts suggests that sulla honey is quite distinctive relative to the other honeys that have been chemically studied by GC\/MS, but no specific markers of the honey botanical origin were found.<\/jats:p>","DOI":"10.3390\/molecules15096375","type":"journal-article","created":{"date-parts":[[2010,9,19]],"date-time":"2010-09-19T09:41:46Z","timestamp":1284889306000},"page":"6375-6385","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Composition of Sulla (Hedysarum coronarium L.) Honey Solvent Extractives Determined by GC\/MS: Norisoprenoids and Other Volatile Organic Compounds"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0727-6662","authenticated-orcid":false,"given":"Igor","family":"Jerkovi\u0107","sequence":"first","affiliation":[{"name":"Faculty of Chemistry and Technology, University of Split, N. Tesle 10\/V, 21000 Split, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo I.G.","family":"Tuberso","sequence":"additional","affiliation":[{"name":"Dipartimento di Tossicologia, Universit\u00e0 di Cagliari, via Ospedale 72, 09124 Cagliari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirko","family":"Gugi\u0107","sequence":"additional","affiliation":[{"name":"Marko Maruli\u0107 Polytechnic in Knin, P. Kre\u0161imira IV 30, 22300 Knin, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragan","family":"Bubalo","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, University of Zagreb, Sveto\u0161imunska 25, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2010,9,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1016\/j.foodchem.2006.07.068","article-title":"A review of volatile analytical methods for determining the botanical origin of honey","volume":"103","author":"Pino","year":"2007","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.ultsonch.2006.12.014","article-title":"Comparison of hydrodistillation and ultrasonic solvent extraction for the isolation of volatile compounds from two unifloral honeys of Robinia pseudoacacia L. and Castanea sativa L.","volume":"14","author":"Klein","year":"2007","journal-title":"Ultrason. Sonochem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1002\/jsfa.1934","article-title":"Evaluation of four isolation techniques for honey aroma compounds","volume":"85","author":"Alissandrakis","year":"2005","journal-title":"J. Sci. Food Agric."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/BF02491721","article-title":"Analysis of volatile compounds of rosemary honey. Comparison of different extraction techniques","volume":"57","author":"Cabezudo","year":"2003","journal-title":"Chromatographia"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/S0308-8146(03)00013-X","article-title":"Ultrasound-assisted extraction of volatile compounds from citrus flowers and citrus honey","volume":"82","author":"Alissandrakis","year":"2003","journal-title":"Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1834","DOI":"10.1021\/jf960625+","article-title":"Composition of Australian honey extractives. 1. Norisoprenoids, monoterpenes, and other natural volatiles from blue gum (Eucalyptus leucoxylon) and yellow box (Eucalyptus melliodora) honeys","volume":"45","author":"Rintoul","year":"1997","journal-title":"J. Agric. Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1021\/jf00051a036","article-title":"Comparison of organic extractives found in leatherwood (Eucryphia lucida) honey and leatherwood flowers and leaves","volume":"43","author":"Rowland","year":"1995","journal-title":"J. Agric. Food Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1080\/00218839.1995.11100890","article-title":"Extractable organic substances from New Zealand unifloral vipers bugloss (Echium vulgare) honey","volume":"34","author":"Wilkins","year":"1995","journal-title":"J. Apic. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1021\/jf00089a004","article-title":"Extractives from New Zealand unifloral honeys. 2. Degraded carotenoids and other substances from heather honey","volume":"37","author":"Tan","year":"1989","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1021\/jf00081a012","article-title":"Extractives from New Zealand honeys. 1. White clover, manuka, and manuka unifloral honeys","volume":"36","author":"Tan","year":"1988","journal-title":"J. Agric. Food Chem."},{"key":"ref_11","first-page":"90","article-title":"Volatile compounds in floral honeys","volume":"26","author":"Moreira","year":"2003","journal-title":"Quim. Nova"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1016\/j.foodchem.2006.10.020","article-title":"Occurrence of 2-hydroxy-5-methyl-3-hexanone and 3-hydroxy-5-methyl-2-hexanone as indicators of botanic origin in eucalyptus honeys","volume":"103","author":"Sanz","year":"2007","journal-title":"Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1021\/jf970510l","article-title":"Floral origin markers of chestnut and lime tree honeys","volume":"46","author":"Guyot","year":"1998","journal-title":"J. Agric. Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0308-8146(98)00122-8","article-title":"Floral origin markers of heather honeys: Calluna vulgaris and Erica arborea","volume":"64","author":"Guyot","year":"1999","journal-title":"Food Chem."},{"key":"ref_15","first-page":"89","article-title":"The effect of utilization frequency on the forage production of sulla (Hedysarum coronarium L.)","volume":"2","author":"Sulas","year":"1997","journal-title":"Ital. J. Agron."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1002\/(SICI)1097-0010(200003)80:4<507::AID-JSFA558>3.0.CO;2-5","article-title":"Invertase activity in fresh and processed honeys","volume":"80","author":"Torrento","year":"2000","journal-title":"J. Sci. Food Agric."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.aca.2004.01.013","article-title":"Supervised pattern recognition applied to the discrimination of the floral origin of six types of Italian honey samples","volume":"515","author":"Marini","year":"2004","journal-title":"Anal. Chim. Acta"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Winterhalter, P., and Russell, L. (2002). Carotenoid-derived aroma compounds, American Chemical Society.","DOI":"10.1021\/bk-2002-0802"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1146\/annurev.arplant.56.032604.144046","article-title":"Abscisic acid biosynthesis and catabolism","volume":"56","author":"Nambara","year":"2005","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.foodchem.2004.03.009","article-title":"Volatile norisoprenoids as markers of botanical origin of Sardinian strawberry-tree (Arbutus unedo L.) honey: Characterisation of aroma compounds by dynamic headspace extraction and gas chromatography-mass spectrometry","volume":"89","author":"Bianchi","year":"2005","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1021\/jf60224a046","article-title":"Volatile constituents of some unifloral Australian honeys","volume":"27","author":"Graddon","year":"1979","journal-title":"J. Agric. Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.3390\/molecules15042911","article-title":"Organic extractives from Mentha spp. honey and the bee-stomach: methyl syringate, vomifoliol, terpenediol I, hotrienol and other compounds","volume":"15","author":"Bubalo","year":"2010","journal-title":"Molecules"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4486","DOI":"10.1073\/pnas.0836984100","article-title":"Isolation and identification of new components of the honey bee (Apis mellifera L.) queen retinue pheromone","volume":"100","author":"Keeling","year":"2003","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1021\/jf803991j","article-title":"Methyl syringate: a chemical marker of asphodel (Asphodelus microcarpus Salzm","volume":"57","author":"Tuberoso","year":"2009","journal-title":"et Viv.) monofloral honey J. Agric. Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1002\/cbdv.200900100","article-title":"Contribution of the bees and combs to honey volatiles: blank-trial probe for honey biodiversity chemical profiling","volume":"7","year":"2010","journal-title":"Chem. Biodivers."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1080\/0005772X.1978.11097714","article-title":"Methods of melissopalynology","volume":"59","author":"Louveaux","year":"1978","journal-title":"Bee World"},{"key":"ref_27","unstructured":"El-Sayed, A.M. (2010, June 21). The pherobase: database of insect pheromones and semiochemicals. http:\/\/www.pherobase.com\/."}],"updated-by":[{"DOI":"10.3390\/molecules181113434","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2010,9,9]],"date-time":"2010-09-09T00:00:00Z","timestamp":1283990400000}}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/15\/9\/6375\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T06:50:43Z","timestamp":1754290243000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/15\/9\/6375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,9]]},"references-count":27,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2010,9]]}},"alternative-id":["molecules15096375"],"URL":"https:\/\/doi.org\/10.3390\/molecules15096375","relation":{"correction":[{"id-type":"doi","id":"10.3390\/molecules181113434","asserted-by":"object"}]},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,9]]}}}