{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:55:16Z","timestamp":1791269716444,"version":"4.1.0"},"reference-count":28,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2011,1,27]],"date-time":"2011-01-27T00:00:00Z","timestamp":1296086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Three anthraquinones\u2014emodin, chrysophanol, and physcion\u2014were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n-hexane\u2013ethanol\u2013water (18:22:3, v\/v\/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by 1H-NMR and 13C-NMR analysis. This is the first time these anthraquinones have been obtained from R. japonicus by HSCCC.<\/jats:p>","DOI":"10.3390\/molecules16021201","type":"journal-article","created":{"date-parts":[[2011,1,31]],"date-time":"2011-01-31T11:44:06Z","timestamp":1296474246000},"page":"1201-1210","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography"],"prefix":"10.3390","volume":"16","author":[{"given":"Shuying","family":"Guo","sequence":"first","affiliation":[{"name":"College of Pharmacy, Jilin Medical College, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Jilin Medical College, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruonan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Phytochemistry, Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiankang","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Jilin Medical College, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Phytochemistry, Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2011,1,27]]},"reference":[{"key":"ref_1","first-page":"1251","article-title":"Anthraquinones from the roots of Prismatomeris tetrandra","volume":"5","author":"Zhang","year":"2010","journal-title":"Nat. Prod. Commun."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1002\/hlca.200900460","article-title":"Anthraquinones and lignans from Cassia occidentalis","volume":"93","author":"Li","year":"2010","journal-title":"Helv. Chim. Acta"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.fitote.2009.08.009","article-title":"A novel antifungal anthraquinone from seeds of Aegle marmelos Correa (family Rutaceae)","volume":"81","author":"Mishra","year":"2010","journal-title":"Fitoterapia"},{"key":"ref_4","unstructured":"(1977). Dictionary of Chinese Medicine, Shanghai Science and Technology Press."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6436","DOI":"10.3390\/molecules15096436","article-title":"Anti-inflammatory activity of chrysophanol through the suppression of NF-\u03baB\/caspase-1 activation in vitro and in vivo","volume":"15","author":"Kim","year":"2010","journal-title":"Molecules"},{"key":"ref_6","first-page":"1508","article-title":"Antibacterial activity of chrysophanol isolated from Aloe excelsa (Berger)","volume":"5","author":"Coopoosamy","year":"2006","journal-title":"Afr. J. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1111\/j.1742-7843.2009.00389.x","article-title":"Investigations of free anthraquinones from rhubarb against \u03b1-naphthylisothiocyanate-induced cholestatic liver injury in rats","volume":"104","author":"Zhao","year":"2009","journal-title":"Basic Clin. Pharmcol. Toxicol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1080\/10286020412331286434","article-title":"PTP1B inhibitor from Saussrurea Lappa","volume":"8","author":"Li","year":"2006","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1002\/med.20094","article-title":"Anti-cancer properties of anthraquinones from rhubarb","volume":"27","author":"Huang","year":"2007","journal-title":"Med. Res. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1111\/j.2042-7158.2010.01151.x","article-title":"Anti-inflammatory and anticancer compounds isolated from Ventilago madraspatana Gaertn., Rubia cordifolia Linn. and Lantana camara Linn","volume":"62","author":"Ghosh","year":"2010","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.jep.2008.11.008","article-title":"Anti-angiogenic effects of rhubarb and its anthraquinone derivatives","volume":"121","author":"He","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1248\/bpb.30.2207","article-title":"anthraquinones from the seeds of Cassia tora with inhibitory activity on protein glycation and aldose reductase","volume":"30","author":"Jang","year":"2007","journal-title":"Biol. Pharm. Bull."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.jep.2009.04.062","article-title":"Antimocribial activities of methanol extract and compounds from stem bark of Vismia rubescens","volume":"124","author":"Tamokou","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/S0021-9673(00)80907-3","article-title":"Efficient preparative counter-current chromatography with a coil planet centrifuge","volume":"214","author":"Ito","year":"1981","journal-title":"J. Chromatogr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.seppur.2010.02.012","article-title":"Separation of flavonol glycosides from Flaveria bidentis (L.) Kuntze by high-speed counter-current chromatography","volume":"72","author":"Xie","year":"2010","journal-title":"Sep. Purif. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.seppur.2010.09.010","article-title":"Application of aqueous two-phase flotation in the separation and concentration of puerarin from Puerariae extract","volume":"75","author":"Bi","year":"2010","journal-title":"Sep. Purif. Technol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.seppur.2007.05.011","article-title":"Separation and purification of DL-tetrahydropalmatine from Corydalis yanhusuo by high-speed counter-current chromatography","volume":"58","author":"Liu","year":"2008","journal-title":"Sep. Purif. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1002\/pca.1152","article-title":"Application of microwave-assisted extraction coupled with high-speed counter-current chromatography for separation and purification of dehydrocavidine from Corydalis saxicola Bunting","volume":"20","author":"Deng","year":"2009","journal-title":"Phytochem. Anal."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1002\/pca.1116","article-title":"Preparative isolation of six major saponins from Platycodi Radix by high-speed counter-current chromatography","volume":"20","author":"Ha","year":"2009","journal-title":"Phytochem. Anal."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1556\/AChrom.20.2008.2.11","article-title":"Separation of two main triterpene glycosides from sea cucumber Pearsonothuria graeffei by high-speed countercurrent chromatography","volume":"20","author":"Dong","year":"2008","journal-title":"Acta Chromatographica"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.seppur.2008.07.011","article-title":"Preparative separation and purification of five anthraquinones from Cassia tora L. by high-speed counter-current chromatography","volume":"63","author":"Zhu","year":"2008","journal-title":"Sep. Purif. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.seppur.2009.09.009","article-title":"Application of high-speed counter-current chromatography and preparative high-performance liquid chromatography mode for rapid isolation of anthraquinones from Morinda officinalis How","volume":"70","author":"Zhu","year":"2009","journal-title":"Sep. Purif. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.seppur.2010.08.007","article-title":"Separation of minor coumarins from Peucedanum praeruptorum using HSCCC and preparative HPLC guided by HPLC\/MS","volume":"75","author":"Hou","year":"2010","journal-title":"Sep. Purif. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.chroma.2004.08.101","article-title":"Preparative isolation and purification of hydroxyanthraquinones and cinnamic acid from the Chinese medical herb Rheum officinale Baill. by high-speed counter-current chromatography","volume":"1052","author":"Liu","year":"2004","journal-title":"J. Chromatogr. A"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0021-9673(99)00827-4","article-title":"Preparative isolation and purification of hydroxyanthraquinones from Rheum officinale Baill by high-speed counter-current chromatography using pH-modulated stepwise elution","volume":"858","author":"Yang","year":"1999","journal-title":"J. Chromatogr. A"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/S0021-9673(00)94500-X","article-title":"Separation of hydroxyanthraquinones derivatives extracted from rheum with analytical high-speed counter-current chromatography","volume":"442","author":"Zhang","year":"1988","journal-title":"J. Chromatogr."},{"key":"ref_27","first-page":"321","article-title":"Studies on the anthraquinones of Hotao Rhubarb (Pheum hotaoense)","volume":"31","author":"Li","year":"2000","journal-title":"Chin. Tradit. Herb. Drugs"},{"key":"ref_28","first-page":"5","article-title":"A new indole derivative isolated from the root of Tuber Fleeceflower (Polygonum multiflorum)","volume":"29","author":"Yang","year":"1998","journal-title":"Chin. Tradit. Herb. Drugs"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/16\/2\/1201\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:55:05Z","timestamp":1760219705000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/16\/2\/1201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1,27]]},"references-count":28,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2011,2]]}},"alternative-id":["molecules16021201"],"URL":"https:\/\/doi.org\/10.3390\/molecules16021201","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1,27]]}}}