{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:16:36Z","timestamp":1790709396997,"version":"4.1.0"},"reference-count":54,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2011,7,26]],"date-time":"2011-07-26T00:00:00Z","timestamp":1311638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Rhinacanthus nasutus (L.) Kurz (Acanthaceae) is an herb native to Thailand and Southeast Asia, known for its antioxidant properties. Hypoxia leads to an increase in reactive oxygen species in cells and is a leading cause of neuronal damage. Cell death caused by hypoxia has been linked with a number of neurodegenerative diseases including some forms of dementia and stroke, as well as the build up of reactive oxygen species which can lead to diseases such as Huntington\u2019s disease, Parkinson\u2019s disease and Alzeheimer\u2019s disease. In this study we used an airtight culture container and the Mitsubishi Gas Company anaeropack along with the MTT assay, LDH assay and the trypan blue exlusion assay to show that 1 and 10 \u00b5g mL\u22121 root extract of R. nasutus is able to significantly prevent the death of HT-22 cells subjected to hypoxic conditions, and 0.1 to 10 \u00b5g mL\u22121 had no toxic effect on HT-22 under normal conditions, whereas 100 \u00b5g mL\u22121 reduced HT-22 cell proliferation. We also used H2DCFDA staining to show R. nasutus can reduce reactive oxygen species production in HT-22 cells.<\/jats:p>","DOI":"10.3390\/molecules16086322","type":"journal-article","created":{"date-parts":[[2011,7,26]],"date-time":"2011-07-26T11:29:34Z","timestamp":1311679774000},"page":"6322-6338","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Rhinacanthus nasutus Protects Cultured Neuronal Cells against Hypoxia Induced Cell Death"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2074-8262","authenticated-orcid":false,"given":"James M.","family":"Brimson","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, 154 Rama I Rd, Pathumwan, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4177-2165","authenticated-orcid":false,"given":"Tewin","family":"Tencomnao","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, 154 Rama I Rd, Pathumwan, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2011,7,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1254\/jphs.10134FP","article-title":"Activation of dopamine D4 receptors is protective against hypoxia\/reoxygenation-induced cell death in HT22 cells","volume":"114","author":"Shimada","year":"2010","journal-title":"J. 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