{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:45:28Z","timestamp":1791269128983,"version":"4.1.0"},"reference-count":21,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2011,8,29]],"date-time":"2011-08-29T00:00:00Z","timestamp":1314576000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The increased incidence of Multidrug-Resistant Mycobacterium tuberculosis (MDR-MT) requires the search for alternative antimycobacterial drugs. The main aim of this study was to evaluate the dichloromethane extract from Aristolochia brevipes (Rhizoma) and the compounds isolated from this extract against several mycobacterial strains, sensitive, resistant (monoresistant), and clinical isolates (multidrug-resistant), using the alamarBlue\u2122 microassay. The extract was fractionated by column chromatography, yielding the following eight major compounds: (1) 6\u03b1-7-dehydro-N-formylnornantenine; (2) E\/Z-N-formylnornantenine; (3) 7,9-dimethoxytariacuripyrone; (4) 9-methoxy-tariacuripyrone; (5) aristololactam I; (6) \u03b2-sitosterol; (7) stigmasterol; and (8) 3-hydroxy-\u03b1-terpineol. The structures of these compounds were elucidated by 1H- and 13C- (1D and 2D) Nuclear Magnetic Resonance (NMR) spectroscopy. This study demonstrates that the dichloromethane extract (rhizome) of A. brevipes possesses strong in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv (Minimum Inhibitory Concentration value [MIC], 12.5 \u00b5g\/mL). The most active compound against all mycobacterial strains tested was the compound aristolactam I (5), with MIC values ranging between 12.5 and 25 \u00b5g\/mL. To our knowledge, this the first report of antimycobacterial activity in this plant.<\/jats:p>","DOI":"10.3390\/molecules16097357","type":"journal-article","created":{"date-parts":[[2011,8,30]],"date-time":"2011-08-30T06:04:23Z","timestamp":1314684263000},"page":"7357-7364","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Antibacterial Activity of Aristolochia brevipes against Multidrug-Resistant Mycobacterium tuberculosis"],"prefix":"10.3390","volume":"16","author":[{"given":"V\u00edctor Manuel","family":"Navarro-Garc\u00eda","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Biomedical Research Center of the South (IMSS), Argentina 1, Col. Centro, 62790 Xochitepec, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julieta","family":"Luna-Herrera","sequence":"additional","affiliation":[{"name":"Immunochemistry Laboratory II, Department of Immunology, National School of Sciences Bilogy (IPN), Prolongaci\u00f3n Carpio y Plan de Ayala S\/N, Col. Casco de Santo Tom\u00e1s, 11340 Mexico, D.F., Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma. Gabriela","family":"Rojas-Bribiesca","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Biomedical Research Center of the South (IMSS), Argentina 1, Col. Centro, 62790 Xochitepec, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"\u00c1lvarez-Fitz","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Biomedical Research Center of the South (IMSS), Argentina 1, Col. Centro, 62790 Xochitepec, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Yolanda","family":"R\u00edos","sequence":"additional","affiliation":[{"name":"Chemical Research Center, University of Morelos (UAEM), Av. Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2011,8,29]]},"reference":[{"key":"ref_1","unstructured":"(2009). Tuberculosis Facts, 2009 Update."},{"key":"ref_2","unstructured":"(2005). Quinto Informe de la Secretar\u00eda de Salud P\u00fablica M\u00e9xico, Subsecretar\u00eda de Prevenci\u00f3n y Promoci\u00f3n a la Salud. Chapter 3."},{"key":"ref_3","first-page":"1","article-title":"Tuberculosis in developing countries: Burden, intervention and cost","volume":"65","author":"Murray","year":"1990","journal-title":"Bull. Int. Union against Tuberculosis Lung Dis."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Barstian, I., and Portaels, F. (2000). In Multidrug-resistant Tuberculosis, Kluwer Academic. [1st].","DOI":"10.1007\/978-94-011-4084-3"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1039\/b212154a","article-title":"Antimycobacterial natural products","volume":"20","author":"Copp","year":"2003","journal-title":"Nat. Prod. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1039\/a902202c","article-title":"The influence of natural products upon drug discovery","volume":"17","author":"Newman","year":"2000","journal-title":"Nat. Prod. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1021\/np030096l","article-title":"Natural products as sources of new drugs over the period 1981-2002","volume":"66","author":"Newman","year":"2003","journal-title":"J. Nat. Prod."},{"key":"ref_8","unstructured":"Mart\u00ednez, M. (1991). Las Plantas Medicinales de M\u00e9xico, Botas. [6th]."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1039\/b401950d","article-title":"Terpenoids of Aristolochia and their biological activities","volume":"21","author":"Amooru","year":"2004","journal-title":"Nat. Prod. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1021\/np50085a011","article-title":"9-methoxy-and 7,9-dimethoxytariacuripyrone, natural nitro-compounds with a new basic skeleton from Aristolochia brevipes","volume":"55","author":"Achenbach","year":"1992","journal-title":"J. Nat. Prod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1055\/s-2006-958049","article-title":"6a,7-dehydro-N-formylnornatenine and other constituents from Aristolochia brevipes","volume":"61","author":"Achenbach","year":"1995","journal-title":"Planta Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1021\/np50063a032","article-title":"N-Formylnornantenine, a new aporphine alkaloid from Cyclea atjehensis","volume":"52","author":"Tantisewie","year":"1989","journal-title":"J. Nat. Prod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1021\/np50032a017","article-title":"Studies on Aristolochia III. Isolation and biological evaluation of constituents of Aristolochia indica roots for fertility-regulation activity","volume":"47","author":"Che","year":"1984","journal-title":"J. Nat. Prod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1021\/np960710b","article-title":"New protopine and benzyltetrahydroprotoberberine alkaloids from Aristolochia constricta and their activity on isolated guinea-pig ileum","volume":"60","author":"Rastrelli","year":"1997","journal-title":"J. Nat. Prod."},{"key":"ref_15","unstructured":"Tian-shung, W., Amooru, D., Chung-Ren, S., and Ping-Chung, K. (2005). Studies in Natural Products Chemistry. Bioactive Natural Products (Part L), Elsevier."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1093\/mutage\/9.6.547","article-title":"9-methoxytariacuripyrone, a naturally occurring nitro-aromatic compound with strong mutagenicity in Salmonella typhimurium","volume":"9","author":"Schimmer","year":"1994","journal-title":"Mutagenesis"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.1016\/S0040-4039(01)91980-X","article-title":"Four new fluorescent components isolated from the callus tissue of Stephania cepharanta","volume":"15","author":"Akasu","year":"1974","journal-title":"Tetrahedron Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/S0031-9422(00)89394-2","article-title":"The structure of alkaloid Y from Schefferomitra subaequalis. (Abstract)","volume":"17","author":"Dyke","year":"1978","journal-title":"Phytochemistry"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/S0031-9422(00)90492-8","article-title":"Aristolochic acids, aristolactam alkaloids and amides from Aristolochia kankauensis","volume":"36","year":"1994","journal-title":"Phytochemistry"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.1135\/cccc19952189","article-title":"Cytochrome P-450 and peroxidase oxidize detoxication products of carcinogenic aristolochic acids (aristolactams) to reactive metabolites binding to DNA in vitro","volume":"60","author":"Stiboroba","year":"1995","journal-title":"Collect Czech Chem. Commun."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1093\/jac\/dkl523","article-title":"Synergistic antimycobacterial activities of sesquiterpene lactones from Laurus spp","volume":"59","author":"Costa","year":"2007","journal-title":"J. Antimicrob. Chemother."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/16\/9\/7357\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:57:13Z","timestamp":1760219833000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/16\/9\/7357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,8,29]]},"references-count":21,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2011,9]]}},"alternative-id":["molecules16097357"],"URL":"https:\/\/doi.org\/10.3390\/molecules16097357","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,8,29]]}}}