{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T21:44:59Z","timestamp":1783979099223,"version":"3.55.0"},"reference-count":101,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2012,2,21]],"date-time":"2012-02-21T00:00:00Z","timestamp":1329782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Shigellosis is an important cause of worldwide morbidity and mortality among young children and old people for which treatment with antimicrobial agents is limited. Hence, the need for curative potentials obtainable from medicinal plants becomes inevitable. This study was carried out to assess the antibacterial potentials of crude methanolic extract of the stem bark of Acacia mearnsii against some selected bacteria of clinical importance in shigellosis. The bacteria were inhibited by the extract to produce concentration dependent inhibition zones. The extract exhibited a varied degree of antibacterial activity against all the tested isolates. The MIC values for Gram negative (0.0391\u20130.3125) mg\/mL and those of Gram positive bacteria (0.0781\u20130.625) mg\/mL indicated that the Gram negative bacteria were more inhibited by the extract than the Gram positive bacteria. Average log reduction in viable cell count in time-kill assay ranged between \u22122.456 Log10 to 2.230 Log10 cfu\/mL after 4 h of interaction, and between \u22122.921 Log10 and 1.447 Log10 cfu\/mL after 8 h interaction in 1\u00d7 MIC and 2\u00d7 MIC of the extract. The study provided scientific justification for the use of the crude methanolic extract from the stem bark of A. mearnsii in shigellosis. The degree of the antibacterial activity indicated that the crude extract is a potential source of bioactive compounds that could be useful for the development of new antimicrobial agents capable of decreasing the burden of drug resistance and cost of management of diseases of clinical and public health importance in South Africa.<\/jats:p>","DOI":"10.3390\/molecules17022103","type":"journal-article","created":{"date-parts":[[2012,2,21]],"date-time":"2012-02-21T11:10:48Z","timestamp":1329822648000},"page":"2103-2118","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["In Vitro Antibacterial and Time-Kill Assessment of Crude Methanolic Stem Bark Extract of Acacia mearnsii De Wild against Bacteria in Shigellosis"],"prefix":"10.3390","volume":"17","author":[{"given":"Olufunmiso Olusola","family":"Olajuyigbe","sequence":"first","affiliation":[{"name":"Phytomedicine Research Centre, Department of Botany, University of Fort Hare, Alice, 5700, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony Jide","family":"Afolayan","sequence":"additional","affiliation":[{"name":"Phytomedicine Research Centre, Department of Botany, University of Fort Hare, Alice, 5700, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2012,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1590\/S0037-86822011005000051","article-title":"Antimicrobial Resistance of Shigella spp. isolated in the State of Par\u00e1, Brazil","volume":"44","author":"Bastos","year":"2011","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_2","first-page":"553","article-title":"Ceftriaxone resistant Shigella flexneri, an emerging problem","volume":"64","author":"Gupta","year":"2010","journal-title":"Indian J. Med. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.1128\/JCM.43.6.2608-2612.2005","article-title":"Outbreak of dysentery associated with Ceftriaxone-resistant Shigella sonnei: First report of Plasmid-Mediated CMY-2-Type AmpC \u00df-Lactamase resistance in S. sonnei","volume":"43","author":"Huang","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1053\/j.spid.2004.07.005","article-title":"Shigella infections in children: new insights","volume":"15","author":"Ashkenazi","year":"2004","journal-title":"Semin. Pediatr. Infect. Dis."},{"key":"ref_5","first-page":"17","article-title":"Search for shiga toxin producing Escherichia coli (STEC) including 0157: H7 strains in and around Kolkata","volume":"21","author":"Chattopadhyay","year":"2003","journal-title":"Indian J. Med. Microbiol."},{"key":"ref_6","first-page":"341","article-title":"Antimicrobial activity of Thalictrum javanicum root extract against certain human pathogen","volume":"7","author":"Tayung","year":"2005","journal-title":"J. Curr. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1111\/j.1574-695X.2011.00810.x","article-title":"Development of a new guinea-pig model of shigellosis.","volume":"62","author":"Barman","year":"2011","journal-title":"FEMS Immunol. Med. Mic."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e1264","DOI":"10.1371\/journal.pntd.0001264","article-title":"A multi-center randomized trial to assess the efficacy of gatifloxacin versus ciprofloxacin for the treatment of shigellosis in Vietnamese children","volume":"5","author":"Vinh","year":"2011","journal-title":"PLoS Negl. Trop. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1089\/fpd.2008.0109","article-title":"Shigella from humans in Thailand during 1993 to 2006: Spatial-time trends in species and serotype distribution.","volume":"5","author":"Bangtrakulnonth","year":"2008","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1056\/NEJM198303243081203","article-title":"Haemorrhagic colitis associated with a rare Escherichia coli serotype","volume":"308","author":"Riley","year":"1983","journal-title":"N. Engl. J. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nrmicro818","article-title":"Pathogenic Escherichia coli","volume":"2","author":"Kaper","year":"2004","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1017\/S0950268807009351","article-title":"Part II. Analysis of data gaps pertaining to Shigella infections in low and medium human development index countries, 1984-2005","volume":"136","author":"Ram","year":"2008","journal-title":"Epidemiol. Infect."},{"key":"ref_13","first-page":"651","article-title":"Global burden of Shigella infections: implications for vaccine development and implementation of control strategies","volume":"77","author":"Kotloff","year":"1999","journal-title":"Bull. World Health Organ."},{"key":"ref_14","first-page":"133","article-title":"Shigellosis.","volume":"43","author":"Niyogi","year":"2005","journal-title":"J. Microbiol."},{"key":"ref_15","unstructured":"(1998). Vaccines, Immunization and Biologicals, World Health Organization."},{"key":"ref_16","first-page":"6","article-title":"Prevalence and pattern of antimicrobial resistance of Shigella species among patients with acute diarrhoea in Karaj, Tehran, Iran","volume":"21","author":"MoezArdalan","year":"2003","journal-title":"J. Health Popul. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1017\/S0950268804003590","article-title":"Shigellosis remains an important problem in children less than 5 years of age in Thailand.","volume":"133","author":"Lee","year":"2005","journal-title":"Epidemiol. Infect."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"561","DOI":"10.2471\/BLT.05.023853","article-title":"Trend and disease burden of bacillary dysentery in china (1991-2000)","volume":"84","author":"Wang","year":"2006","journal-title":"Bull. World Health Organ."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"416","DOI":"10.4269\/ajtmh.2005.73.416","article-title":"Occurrence of shigellosis in the young and elderly in rural China: results of a 12-month population-based surveillance study","volume":"73","author":"Wang","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1007\/BF02898447","article-title":"Current status of anti-diarrheal and anti-secretory drugs in the management of acute childhood diarrhoea","volume":"73","author":"Alam","year":"2006","journal-title":"Indian J. Pediatr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1099\/jmm.0.46441-0","article-title":"Molecular characterization of antimicrobial resistance in Shigella sonnei isolates in Korea","volume":"55","author":"Seol","year":"2006","journal-title":"J. Med. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e353","DOI":"10.1371\/journal.pmed.0030353","article-title":"A multicentre study of Shigella diarrhoea in six Asian countries: disease burden, clinical manifestations, and microbiology","volume":"3","author":"Kim","year":"2006","journal-title":"PLoS Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1128\/jcm.11.4.337-339.1980","article-title":"Foodborne outbreak of shigellosis caused by an unusual Shigella strain","volume":"11","author":"Huq","year":"1980","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","unstructured":"(2002). \n\t\t\t\t\t\t\t\t\t\t\t\t   Shigella\n\t\t\t\t\t\t\t\t\t\t\t\t         ."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1056\/NEJM197107012850101","article-title":"Pathogenesis of Escherichia coli diarrhea","volume":"285","author":"DuPont","year":"1971","journal-title":"N. Engl. J. Med."},{"key":"ref_26","unstructured":"Hansler, W.J., and Shuman, M. (1998). Topley and Wilson\u2019s Microbiology and Microbial Infections, Arnold."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1111\/j.1365-2958.2005.04645.x","article-title":"A secreted anti-activator, OspD1, and its chaperone, Spa15, are involved in the control of transcription by the type III secretion apparatus activity in Shigella flexneri.","volume":"56","author":"Parsot","year":"2005","journal-title":"Mol. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1098\/rsif.2007.1038","article-title":"Time is of the essence: exploring a measles outbreak response vaccination in Niamey, Niger.","volume":"5","author":"Grais","year":"2008","journal-title":"J. R. Soc. Interface"},{"key":"ref_29","first-page":"28","article-title":"An outbreak of shigellosis in central India: higher death rate in post-measles shigellosis.","volume":"7","author":"Mathur","year":"1989","journal-title":"J. Diarrhoeal Dis. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1093\/ajcn\/68.5.1088","article-title":"Predictors of serum retinol in children with shigellosis.","volume":"68","author":"Mitra","year":"1998","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_31","first-page":"1246","article-title":"Reducing deaths from diarrhoea through oral rehydration therapy.","volume":"78","author":"Victora","year":"2000","journal-title":"Bull. World Health Organ."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1746-4269-2-4","article-title":"Ethno-diversity within current ethno-pharmacology as part of Israeli traditional medicine-A review.","volume":"2","author":"Lev","year":"2006","journal-title":"J. Ethnobiol. Ethnomed."},{"key":"ref_33","first-page":"29","article-title":"An ethnobotanical survey of important wild medicinal plants of Hattar district Haripur, Pakistan.","volume":"12","author":"Hussain","year":"2008","journal-title":"Ethnobot. Leafl."},{"key":"ref_34","first-page":"1","article-title":"Medicinal plants: can utilization and conservation coexist? Advances in Economic Botany.","volume":"12","author":"Sheldon","year":"1997","journal-title":"Econ. Bot."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jep.2006.10.032","article-title":"An ethnobotanical survey of medicinal plants used in Loja and Zamora-Chinchipe, Ecuador.","volume":"111","author":"Tene","year":"2007","journal-title":"J. Ethnopharmacol."},{"key":"ref_36","first-page":"37","article-title":"Do not put too much value on conventional medicine.","volume":"100","author":"Hack","year":"2006","journal-title":"J. Ethnopharmacol."},{"key":"ref_37","first-page":"292","article-title":"Traditional knowledge on plants from Toranmal Plateau of Maharastra.","volume":"2","author":"Sharma","year":"2003","journal-title":"Indian J. Tradit. Know."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0378-8741(01)00362-2","article-title":"Further evaluation of Rwandan medicinal plant extracts for their antimicrobial and antiviral activities.","volume":"79","author":"Cos","year":"2002","journal-title":"J. Ethnopharmacol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.jep.2005.06.017","article-title":"In vitro antifungal assay of traditional Argentine medicinal plants","volume":"102","author":"Muschietti","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.fitote.2004.12.009","article-title":"Antibacterial properties of essential oils from Thai medicinal plants.","volume":"76","author":"Wannissorn","year":"2005","journal-title":"Fitoterapia"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3760","DOI":"10.5897\/AJMR11.627","article-title":"Antibacterial and phytochemical screening of crude ethanolic extracts of Waltheria indica Linn","volume":"5","author":"Olajuyigbe","year":"2011","journal-title":"Afr. J. Microbiol. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0378-8741(95)01287-N","article-title":"Analgesic activity of Psychotria colorata (Willd Ext. & S) Muell. Arg. alkaloid","volume":"48","author":"Elisabetsky","year":"1995","journal-title":"J. Ethnopharmcol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1093\/ecam\/neh140","article-title":"Ayurveda and traditional Chinese medicine: A comparative overview","volume":"2","author":"Patwardhan","year":"2005","journal-title":"Evid.-Based Compl. Alt."},{"key":"ref_44","unstructured":"Latz, P. (2001). Bushfires and Bushtuckers: Aboriginal Plant Use in Central Australia, IAD Press."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1006\/jare.1994.1064","article-title":"The utilization of Australian species of Acacia, with particular reference to those of the subtropical dry zone.","volume":"27","author":"Thomson","year":"1994","journal-title":"J. Arid. Environ."},{"key":"ref_46","unstructured":"Lithgow, M.G. (1997). 60 Wattles of the Chinchilla and Murilla Shires, Private publication."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1086\/381972","article-title":"Clinical relevance of bacteriostatic  versus bactericidal mechanisms of action in the treatment of Gram positive bacterial infections","volume":"38","author":"Pankey","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1093\/clind\/15.5.824","article-title":"\u03b2-Lactamase resistance in gram-negative bacteria: global trends and clinical impact","volume":"15","author":"Sanders","year":"1992","journal-title":"Clin. Infect. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/S1201-9712(03)90042-7","article-title":"Bacteremic pneumococcal infections in immunocompromised patients without AIDS: the impact of beta-lactam resistance on mortality.","volume":"7","author":"Fernandez","year":"2003","journal-title":"Int. J. Infect. Dis."},{"key":"ref_50","first-page":"41","article-title":"Bacteriological examination of chronic osteomyelitis cases in Ile-Ife, South Western Nigeria.","volume":"4","author":"Ikem","year":"2003","journal-title":"Afr. J. Clin. Exp. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/S1473-3099(02)00227-X","article-title":"Secrets of success of a human pathogen: molecular evolution of pandemic clones of methicillin resistant Staphylococcus aureus","volume":"2","author":"Oliveira","year":"2002","journal-title":"Lancet Infect. Dis."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1086\/381972","article-title":"Clinical Relevance of Bacteriostatic versus Bactericidal mechanisms of action in the treatment of Gram positive bacterial infections","volume":"38","author":"Pankey","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2050","DOI":"10.1128\/AAC.41.9.2050","article-title":"Bactericidal activity against intermediately cephalosporin-resistant Streptococcus pneumoniae in cerebrospinal fluid of children with bacterial meningitis treated with high doses of cefotaxime and vancomycin","volume":"41","author":"Doit","year":"1997","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1128\/AAC.41.9.1888","article-title":"Cerebrospinal fluid bactericidal activity against cephalosporin-resistant Streptococcus pneumoniae in children with meningitis treated with high-dose cefotaxime","volume":"41","author":"Friedland","year":"1997","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/0002-9343(84)90662-4","article-title":"Significance of serum bactericidal activity in Gram negative bacillary bacteremia in patients with and without granulocytopenia.","volume":"76","author":"Sculier","year":"1984","journal-title":"Am. J. Med."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1128\/CMR.5.4.420","article-title":"Tests for bactericidal effects of antimicrobial agents: technical performance and clinical relevance.","volume":"5","author":"Peterson","year":"1992","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_57","unstructured":"(1998). Methods for determining bactericidal activity of antimicrobial agents; Approved guideline M26-A, National Committee for Clinical Laboratory Standards."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0378-8741(03)00006-0","article-title":"Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L","volume":"85","author":"Karaman","year":"2003","journal-title":"J. Ethnopharmacol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-8741(97)00123-2","article-title":"Which extractant should be used for the screening and isolation of antimicrobial components from plants?","volume":"60","author":"Eloff","year":"1998","journal-title":"J. Ethnopharmacol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"173","DOI":"10.3923\/ijp.2010.173.182","article-title":"Antibacterial activity, bioavailability and acute toxicity evaluation of the leaf extract of Alchornea cordifolia (Euphorbiaceae)","volume":"6","author":"Gatsing","year":"2010","journal-title":"Int. J. Pharmacol."},{"key":"ref_61","unstructured":"Carbonnelle, B., Denis, F., Marmonier, A., Pinon, G., and Vague, R. (1987). Bacteriologie m\u00e9dicale: Techniques usuelles, SIMEP."},{"key":"ref_62","first-page":"1","article-title":"Antimicrobials and chemotherapy.","volume":"35","author":"Mims","year":"1993","journal-title":"Med. Microbiol. Rev."},{"key":"ref_63","unstructured":"Haslam, E. (1989). Plant Polyphenols, Cambridge University Press."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/BF02375657","article-title":"Utilization of catechin by Rhizobium sp.","volume":"108","author":"Gajendiran","year":"1990","journal-title":"Plant Soil"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"856","DOI":"10.3923\/ijp.2011.856.861","article-title":"Phytochemical assessment and antioxidant activities of alcoholic and aqueous extracts of Acacia mearnsii De Wild","volume":"7","author":"Olajuyigbe","year":"2011","journal-title":"Int. J. Pharmacol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"41","DOI":"10.17485\/ijst\/2009\/v2i2.5","article-title":"Pharmacological importance of Solanum trilobatum","volume":"2","author":"Doss","year":"2009","journal-title":"Indian J. Sci. Technol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1055\/s-2003-38883","article-title":"New antimicrobial alkaloids from the roots of Polyalthia longifolia var. pendula","volume":"69","author":"Faizi","year":"2003","journal-title":"Planta Med."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/j.1348-0421.2004.tb03521.x","article-title":"Antibacterial activity of hydrolyzable tannins derived from medicinal plants against Helicobacter pylori","volume":"48","author":"Funatogawa","year":"2004","journal-title":"Microbiol. Immunol."},{"key":"ref_69","first-page":"431","article-title":"Antimicrobial activity of flavonoids from Pelargonium radula (Cav.) L\u2019Herit","volume":"55","author":"Pepeljnjak","year":"2005","journal-title":"Acta Pharmaceut."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2056","DOI":"10.1111\/j.1365-2672.2007.03456.x","article-title":"Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamia Risso) peel, a byproduct of the essential oil industry","volume":"103","author":"Mandalari","year":"2007","journal-title":"J. Appl. Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1002\/ptr.1876","article-title":"Antimicrobial activity of saponins from Medicago sp.: Structure-activity relationship","volume":"20","author":"Avato","year":"2006","journal-title":"Phytother. Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1439-0434.2007.01309.x","article-title":"Analysis of the antimicrobial activity of flavonoids and saponins isolated from the shoots of Oats (Avena sativa L.)","volume":"156","author":"Baharaminejad","year":"2007","journal-title":"J. Phytopathol."},{"key":"ref_73","unstructured":"Murray, P., Baron, E., Pfaller, M., Tenover, F., and Yolken, R. (1995). Manual of Clinical Microbiology, ASM Press."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0254-6299(15)30140-X","article-title":"Chemical analysis and antimicrobial activity of essential oil extracted from Helichrysum aureonitens","volume":"71","author":"Yani","year":"2005","journal-title":"S. Afr. J. Bot."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1080\/14786410801915838","article-title":"Phenolic contents, antioxidant and antibacterial activities of Hymenocardia acida.","volume":"23","author":"Sofidiya","year":"2009","journal-title":"Nat. Prod. Res."},{"key":"ref_76","first-page":"390","article-title":"Chemical composition and antimicrobial activity of the essential oil from Chrysocoma ciliate L. leaves","volume":"3","author":"Afolayan","year":"2009","journal-title":"J. Med. Plants Res."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1007\/BF02818588","article-title":"Gentamicin resistance in clinical strains of Enterobacteriaceae associated with reduced gentamicin uptake.","volume":"43","author":"Adwan","year":"1998","journal-title":"Folia Microbiol."},{"key":"ref_78","first-page":"8","article-title":"Bioactive berry compounds- novel tools against human pathogens.","volume":"67","author":"Nohynek","year":"2004","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0377-8401(03)00041-5","article-title":"The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: a review.","volume":"106","author":"Min","year":"2003","journal-title":"Anim. Feed Sci.Tech."},{"key":"ref_80","first-page":"673","article-title":"Toxicity of tannic compounds to microorganisms. Plants Polyphenols: Synthesis, Properties, Significance","volume":"59","author":"Field","year":"1992","journal-title":"Basic Life Sci."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0378-8741(03)00191-0","article-title":"Osmitopsis asteriscoides (Asteraceae)-the antimicrobial and essential oil composition of Cape-Dutch remedy","volume":"88","author":"Viljoen","year":"2003","journal-title":"J. Ethnopharmacol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.phytochem.2004.02.011","article-title":"Antimicrobial and antioxidant flavonoids from the roots wood of Bolusathus spesiosus","volume":"65","author":"Erasto","year":"2004","journal-title":"Phytochemistry"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"26","DOI":"10.4103\/0253-7613.13851","article-title":"The potential of aqueous and acetone extracts of galls of Queercus infectoria as antibacterial agents","volume":"37","author":"Basri","year":"2005","journal-title":"Indian J. Pharmacol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1046\/j.1469-0691.2000.00142.x","article-title":"Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by agar dilution.","volume":"6","year":"2000","journal-title":"Clin. Micro. Infect."},{"key":"ref_85","unstructured":"Cheesbrough, M. (2002). Medical Laboratory Manual for Tropical Countries, Tropical health technology publications and Butterworth\u2013Heinemann Ltd. [ELBS]."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/ajcp\/45.4_ts.493","article-title":"Antibiotic susceptibility testing by a standardized single disk method","volume":"45","author":"Bauer","year":"1966","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_87","unstructured":"Wikler, M.A. (2007). Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement, Part M2-A9. M100-S17; C.L.S.I. (Clinical and Laboratory Standard Institute)."},{"key":"ref_88","unstructured":"Wikler, M.A. (2008). Performance Standards for Antimicrobial Susceptibility Testing; Eighteenth Informational Supplement, M100-S18; C.L.S.I. (Clinical and Laboratory Standard Institute)."},{"key":"ref_89","first-page":"181","article-title":"Broth micro-dilution method for determination of susceptibility of Escherichia coli and Staphylococcus aureus to the essential oil of Malaleuca alterifolia (Tea tree oil)","volume":"82","author":"Carson","year":"1995","journal-title":"Microbios"},{"key":"ref_90","first-page":"72","article-title":"Antimicrobial activity of Polygonum tintorium Lour: Extract against oral pathogenic bacteria","volume":"53","author":"Iwaki","year":"2006","journal-title":"Nat. Med."},{"key":"ref_91","first-page":"167","article-title":"Antibacterial, antifungal and cytotoxic activities of Tuberous Roots of Amorphophallus campanulatus","volume":"31","author":"Khan","year":"2007","journal-title":"Turk. J. Biol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1128\/AAC.26.2.214","article-title":"MBCs for Staphylococcus aureus as determined by macrodilution and microdilution techniques","volume":"26","author":"Shanholtzer","year":"1984","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1093\/jac\/15.suppl_A.207","article-title":"Mezlocillin, ceftizoxime, and amikacin alone and in combination against six Enterobacteriaceae in a neutropenic site in rabbits","volume":"15 (Suppl. A)","author":"Gerding","year":"1985","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1093\/jac\/18.1.51","article-title":"Ciprofloxacin, azlocillin, ceftizoxime, and amikacin alone and in combination against Gram negative bacilli in an infected chamber model","volume":"18","author":"Bamberger","year":"1986","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0732-8893(87)90115-5","article-title":"Ceftazidime and amikacin alone and in combination against Pseudomonas aeruginosa and Enterobacteriaceae.","volume":"6","author":"Moody","year":"1987","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_96","first-page":"697","article-title":"Treatment evaluation of experimental staphylococcal infections comparing \u03b2-lactam, lipopeptide, and glycopeptide antimicrobial therapy","volume":"116","author":"Fasching","year":"1990","journal-title":"J. Lab. Clin. Med."},{"key":"ref_97","first-page":"384","article-title":"Control of Aspergillus niger with garlic, onion and leek extracts.","volume":"6","author":"Irkin","year":"2007","journal-title":"Afr. J. Biotechnol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1128\/CMR.1.2.139","article-title":"Antibiotic combinations: should they be tested?","volume":"1","author":"Eliopoulos","year":"1988","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_99","unstructured":"Lorain, V. (1996). Antibiotics in Laboratory Medicine, The Williams & Wilkins Co.. [4th]."},{"key":"ref_100","unstructured":"Cruishank, R., Duguid, J.P., Marmion, B.P., and Swain, R.H.A. (1975). Medical Microbiology. The Practice of Medical Microbiology, Churchill Livingstone. [12th]."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1128\/AAC.38.9.2065","article-title":"Study of comparative antipneumococcal activities of penicillin G, RP 59500, erythromycin, sparfloxacin, ciprofloxacin, and vancomycin by using time-kill methodology","volume":"38","author":"Pankuch","year":"1994","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/17\/2\/2103\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:48:59Z","timestamp":1760219339000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/17\/2\/2103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,21]]},"references-count":101,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2012,2]]}},"alternative-id":["molecules17022103"],"URL":"https:\/\/doi.org\/10.3390\/molecules17022103","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,21]]}}}