{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T08:11:38Z","timestamp":1771488698752,"version":"3.50.1"},"reference-count":40,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:00:00Z","timestamp":1340841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The objective of this study was to evaluate the reactivity of an in-house antigen, extracted from a strain of C. posadasii isolated in northeastern Brazil, by radial immunodiffusion and Western blotting, as well as to establish its biochemical characterization. The protein antigen was initially extracted with the use of solid ammonium sulfate and characterized by 1-D electrophoresis. Subsequently, it was tested by means of double radial immunodiffusion and Western blotting. A positive reaction was observed against the antigen by both immunodiagnostic techniques tested on sera from patients suffering from coccidioidomycosis. Besides this, two immunoreactive protein bands were observed and were revealed to be a \u03b2-glucosidase and a glutamine synthetase after sequencing of the respective N-terminal regions. Our in-house Coccidioides antigen can be promising as a quick and low-cost diagnostic tool without the risk of direct manipulation of the microorganism.<\/jats:p>","DOI":"10.3390\/molecules17077854","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T11:17:49Z","timestamp":1340882269000},"page":"7854-7863","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Biochemical Characterization of an In-House Coccidioides Antigen: Perspectives for the Immunodiagnosis of Coccidioidomycosis"],"prefix":"10.3390","volume":"17","author":[{"given":"Renato Evando Moreira","family":"Filho","sequence":"first","affiliation":[{"name":"Specialized Medical Mycology Center, School of Medicine, Federal University of Cear\u00e1, Fortaleza, CE, CEP, 60430-270, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silviane Praciano","family":"Bandeira","sequence":"additional","affiliation":[{"name":"Specialized Medical Mycology Center, School of Medicine, Federal University of Cear\u00e1, Fortaleza, CE, CEP, 60430-270, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raimunda S\u00e2mia Nogueira","family":"Brillhante","sequence":"additional","affiliation":[{"name":"Specialized Medical Mycology Center, School of Medicine, Federal University of Cear\u00e1, Fortaleza, CE, CEP, 60430-270, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos F\u00e1bio Gadelha","family":"Rocha","sequence":"additional","affiliation":[{"name":"Specialized Medical Mycology Center, School of Medicine, Federal University of Cear\u00e1, Fortaleza, CE, CEP, 60430-270, Brazil"},{"name":"Postgraduate Program in Veterinary Sciences, School of Veterinary Medicine, Cear\u00e1 State University, Fortaleza, CE, CEP, 60740-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilka Maria","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Pici Campus, Fortaleza, CE, 60455-760, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirella Leite","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Pici Campus, Fortaleza, CE, 60455-760, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9bora de Souza Collares Maia","family":"Castelo-Branco","sequence":"additional","affiliation":[{"name":"Specialized Medical Mycology Center, School of Medicine, Federal University of Cear\u00e1, Fortaleza, CE, CEP, 60430-270, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Airton Castro","family":"Rocha","sequence":"additional","affiliation":[{"name":"Specialized Medical Mycology Center, School of Medicine, Federal University of Cear\u00e1, Fortaleza, CE, CEP, 60430-270, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoilo Pires de","family":"Camargo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, S\u00e3o Paulo Federal University, S\u00e3o Paulo, 04021-001, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcio Viana","family":"Ramos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Pici Campus, Fortaleza, CE, 60455-760, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rossana de Aguiar","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Specialized Medical Mycology Center, School of Medicine, Federal University of Cear\u00e1, Fortaleza, CE, CEP, 60430-270, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 J\u00falio Costa","family":"Sidrim","sequence":"additional","affiliation":[{"name":"Specialized Medical Mycology Center, School of Medicine, Federal University of Cear\u00e1, Fortaleza, CE, CEP, 60430-270, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2012,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/s11046-007-9018-7","article-title":"Antifungal susceptibility profiles of Coccidioides immitis and Coccidioides posadasii from endemic and non-endemic areas","volume":"163","author":"Ramani","year":"2007","journal-title":"Mycopathologia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1007\/s10096-008-0508-4","article-title":"Mycelial forms of Coccidioides spp. in the parasitic phase associated to pulmonary coccidioidomycosis with type 2 diabetes mellitus","volume":"27","author":"Zavala","year":"2008","journal-title":"Eur. 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