{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:34:23Z","timestamp":1760243663521,"version":"build-2065373602"},"reference-count":72,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:00:00Z","timestamp":1340841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In vitro high-throughput screening was carried out in order to detect new activities for old drugs and to select compounds for the drug development process comprising new indications. Tebrophen, a known antiviral drug, was found to inhibit activities on inflammation and cancer related targets. In primary screening this semisynthetic halogenated polyphenol was identified to inhibit the activities of kinases ZAP-70 and Lck (IC50 0.34 \u00b5M and 16 \u00b5M, respectively), as well as hydrolase DPPIV (at 80 \u00b5M 41% inhibition). Next, it showed no cytotoxic effects on standard cell lines within 24 h. However, tebrophen slowed propagation of breast cancer (MDA-MB-231), osteosarcoma (U2OS) and cervical carcinoma (HeLa), through at least 35 population doublings in a dose-dependent manner. It completely stopped the division of the prostate cancer (PC3) cell line at 50 \u00b5M concentration and the cells entered massive cell death in less than 20 days. On the other hand, tebrophen did not influence the growth of normal fibroblasts. According to the measured oxidative burst and estimated in silico parameters its direct antioxidative ability is limited. The obtained results indicate that tebrophen can be considered a promising lead molecule for generating more soluble derivatives with specific anticancer efficacy.<\/jats:p>","DOI":"10.3390\/molecules17077864","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T11:17:49Z","timestamp":1340882269000},"page":"7864-7886","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tebrophen \u2014 An Old Polyphenol Drug with Anticancer Potential \u2020"],"prefix":"10.3390","volume":"17","author":[{"given":"Ivica","family":"Rubelj","sequence":"first","affiliation":[{"name":"Laboratory for Molecular and Cellular Biology, Division of Molecular Biology, Ru\u0111er Bo\u0161kovi\u0107 Institute, Bijeni\u010dka cesta 54, HR-10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vi\u0161nja","family":"Stepani\u0107","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline Research Centre Zagreb Ltd., Prilaz baruna Filipovi\u0107a 29, HR-10000 Zagreb, Croatia"},{"name":"Laboratory for Epigenomics, Division of Molecular Medicine, Ru\u0111er Bo\u0161kovi\u0107 Institute, Bijeni\u010dka cesta 54, HR-10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dubravko","family":"Jeli\u0107","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline Research Centre Zagreb Ltd., Prilaz baruna Filipovi\u0107a 29, HR-10000 Zagreb, Croatia"},{"name":"Galapagos istra\u017eiva\u010dki centar d.o.o., Prilaz baruna Filipovi\u0107a 29, HR-10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolina \u0160krobot","family":"Vida\u010dek","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular and Cellular Biology, Division of Molecular Biology, Ru\u0111er Bo\u0161kovi\u0107 Institute, Bijeni\u010dka cesta 54, HR-10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea \u0106uku\u0161i\u0107","family":"Kalaj\u017ei\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular and Cellular Biology, Division of Molecular Biology, Ru\u0111er Bo\u0161kovi\u0107 Institute, Bijeni\u010dka cesta 54, HR-10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milena","family":"Ivankovi\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular and Cellular Biology, Division of Molecular Biology, Ru\u0111er Bo\u0161kovi\u0107 Institute, Bijeni\u010dka cesta 54, HR-10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krunoslav","family":"Nuji\u0107","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline Research Centre Zagreb Ltd., Prilaz baruna Filipovi\u0107a 29, HR-10000 Zagreb, Croatia"},{"name":"Galapagos istra\u017eiva\u010dki centar d.o.o., Prilaz baruna Filipovi\u0107a 29, HR-10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Matija\u0161i\u0107","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline Research Centre Zagreb Ltd., Prilaz baruna Filipovi\u0107a 29, HR-10000 Zagreb, Croatia"},{"name":"University of Zagreb, School of Medicine, Center for Translational and Clinical Research, \u0160alata 2, HR-10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9106-1604","authenticated-orcid":false,"given":"Donatella","family":"Verbanac","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline Research Centre Zagreb Ltd., Prilaz baruna Filipovi\u0107a 29, HR-10000 Zagreb, Croatia"},{"name":"University of Zagreb, School of Medicine, Center for Translational and Clinical Research, \u0160alata 2, HR-10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2012,6,28]]},"reference":[{"key":"ref_1","first-page":"64","article-title":"Novel approach to drug discovery in PLIVA-establishing of HTS Unit and unique Compounds Library","volume":"2","author":"Mesar","year":"2003","journal-title":"Pharmachem"},{"key":"ref_2","first-page":"269","article-title":"Macromolecular Databases\u2014A background of bioinformatics","volume":"41","author":"Toth","year":"2003","journal-title":"Food Technol. Biotech."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s00044-010-9314-5","article-title":"6-Imino-2-thioxo-pyrimidinones as a new class of dipeptidyl peptidase IV inhibitors","volume":"20","author":"Verbanac","year":"2011","journal-title":"Med. Chem. Res."},{"key":"ref_4","first-page":"133","article-title":"Combined in silico and in vitro approach to drug screening","volume":"78","author":"Verbanac","year":"2005","journal-title":"Croat. Chem. Acta"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"253","DOI":"10.2174\/138955707780059853","article-title":"CD26\/dipeptidyl peptidase IV as a novel therapeutic target for cancer and immune disorders","volume":"7","author":"Thompson","year":"2007","journal-title":"Mini Rev. Med. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1096\/fasebj.9.8.7768349","article-title":"Protein kinases 6. The eukaryotic protein kinase superfamily: Kinase (catalytic) domain structure and classification","volume":"9","author":"Hanks","year":"1995","journal-title":"FASEB J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/0092-8674(91)90162-R","article-title":"Regulation of T cell receptor signaling by a src family protein-tyrosine kinase (p59fyn)","volume":"65","author":"Cooke","year":"1991","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1084\/jem.175.6.1483","article-title":"Differential regulation of T cell antigen responsiveness by isoforms of the src-related tyrosine protein kinase p59fyn","volume":"175","author":"Davidson","year":"1992","journal-title":"J. Exp. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S1074-7613(00)80315-5","article-title":"Complex complexes: Signaling at the TCR","volume":"5","author":"Wange","year":"1996","journal-title":"Immunity"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1038\/324674a0","article-title":"Abnormal tyrosine phosphorylation on T-cell receptor in lymphoproliferative disorders","volume":"324","author":"Samelson","year":"1986","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1056\/NEJMp048184","article-title":"Predicting Progression-ZAP-70 in CLL","volume":"351","author":"Hamblin","year":"2004","journal-title":"N. Engl. J. Med."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Nijkamp, F.P., and Parnham, M.J. (2011). Principles of Immunopharmacology, Springer.","DOI":"10.1007\/978-3-0346-0136-8"},{"key":"ref_13","first-page":"367","article-title":"Flavonoids as inhibitors of lck and fyn kinases","volume":"78","author":"Verbanac","year":"2005","journal-title":"Croat. Chem. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1021\/jm0607202","article-title":"Homology modeling of human Fyn kinase structure: discovery of rosmarinic acid as a new Fyn kinase inhibitor and in silico study of its possible binding modes","volume":"50","author":"Mildner","year":"2007","journal-title":"J. Med. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1078\/0171-9335-00302","article-title":"The multifunctional or moonlighting protein CD26\/DPPIV","volume":"82","author":"Boonacker","year":"2003","journal-title":"Eur. J. Cell Biol."},{"key":"ref_16","first-page":"481","article-title":"CD26: A novel treatment target for T-cell lymphoid malignancies?","volume":"22","author":"Sato","year":"2003","journal-title":"Int. J. Oncol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.it.2008.02.010","article-title":"Revisiting an old acquaintance: CD26 and its molecular mechanisms in T cell function","volume":"29","author":"Ohnuma","year":"2008","journal-title":"Trends Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.2741\/2787","article-title":"The role of CD26\/dipeptidyl peptidase IV in cancer","volume":"13","author":"Havre","year":"2008","journal-title":"Front. Biosci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1185\/030079906X162746","article-title":"Inhibition of DPP-4: A new therapeutic approach for the treatment of type 2 diabetes","volume":"23","author":"Pratley","year":"2007","journal-title":"Curr. Med. Res. Opin."},{"key":"ref_20","first-page":"1345","article-title":"CD26\/dipeptidyl peptidase IV and its role in cancer","volume":"19","author":"Pro","year":"2004","journal-title":"Histol. Histopathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.2741\/2799","article-title":"DPP-4 inhibitor therapy: New directions in the treatment of type 2 diabetes","volume":"13","author":"Deacon","year":"2008","journal-title":"Front. Biosci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0079-6468(06)45502-8","article-title":"DPPIV inhibition: Promising therapy for the treatment of type 2 diabetes","volume":"45","author":"Wiedeman","year":"2007","journal-title":"Prog. Med. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0167-0115(93)90435-B","article-title":"Proteolytic processing of neuropeptide Y and peptide YY by dipeptidyl peptidase IV","volume":"49","author":"Mentlein","year":"1993","journal-title":"Regul. Pept."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0167-0115(99)00089-0","article-title":"Dipeptidyl-peptidase IV (CD26)\u2014Role in the inactivation of regulatory peptides","volume":"85","author":"Mentlein","year":"1999","journal-title":"Regul. Pept."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Bansal, P., Paul, P., Mudgal, J., Nayak, G., Thomas, P.S., Priyadarsini, K.I., and Unnikrishnan, M.K. (2011). Antidiabetic, antihyperlipidemic and antioxidant effects of the flavonoid rich fraction of  Pilea microphylla (L.) in high fat diet\/streptozotocin-induced diabetes in mice. Exp. Toxicol. Pathol., in press.","DOI":"10.1016\/j.etp.2010.12.009"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/nsb882","article-title":"Crystal structure of human dipeptidyl peptidase IV\/CD26 in complex with a substrate analog","volume":"10","author":"Rasmussen","year":"2003","journal-title":"Nat. Struct. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"850","DOI":"10.2144\/03354ss02","article-title":"Immunoassay of infectious agents","volume":"35","author":"Andreotti","year":"2003","journal-title":"Biotechniques"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Crowther, J.R. (1995). ELISA: Theory and practice, Humana Press Inc.","DOI":"10.1385\/0896032795"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"12616","DOI":"10.1016\/S0021-9258(18)67135-2","article-title":"Inhibition of the oxidative burst in human neutrophils by sphingoid long-chain bases. Role of protein kinase C in activation of the burst","volume":"261","author":"Wilson","year":"1986","journal-title":"J. Biol. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1093\/gerona\/60.7.820","article-title":"Early-senescing human skin fibroblasts do not demonstrate accelerated telomere shortening","volume":"60","author":"Ferenac","year":"2005","journal-title":"J. Gerontol. A-Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.exger.2010.01.009","article-title":"Abrupt telomere shortening in normal human fibroblasts","volume":"45","author":"Fulgosi","year":"2010","journal-title":"Exp. Gerontol."},{"key":"ref_33","first-page":"3118","article-title":"Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro","volume":"40","author":"Brinkley","year":"1980","journal-title":"Cancer Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/j.1365-2184.2006.00383.x","article-title":"Spontaneous senescence in the MDA-MB-231 cell line","volume":"39","author":"Ferenac","year":"2006","journal-title":"Cell Prolif."},{"key":"ref_35","first-page":"16","article-title":"Establishment and characterization of a human prostatic carcinoma cell line (PC-3)","volume":"17","author":"Kaighn","year":"1979","journal-title":"Invest. Urol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1002\/ijc.2910020505","article-title":"Two established in vitro cell lines from human mesenchymal tumours","volume":"2","author":"Ponten","year":"1967","journal-title":"Int. J. Cancer"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s10522-006-9043-9","article-title":"Telomerase activity in HeLa cervical carcinoma cell line proliferation","volume":"8","author":"Rubelj","year":"2007","journal-title":"Biogerontology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1084\/jem.97.5.695","article-title":"Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix","volume":"97","author":"Scherer","year":"1953","journal-title":"J. Exp. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1159\/000167812","article-title":"Telomerase regulation at the crossroads of cell fate","volume":"122","author":"Sopta","year":"2008","journal-title":"Cytogenet. Genome Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9363","DOI":"10.1073\/pnas.92.20.9363","article-title":"A biomarker that identifies senescent human cells in culture and in aging skin in vivo","volume":"92","author":"Dimri","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1038\/sj.onc.1201862","article-title":"Agents that cause DNA double strand breaks lead to p16INK4a enrichment and the premature senescence of normal fibroblasts","volume":"16","author":"Robles","year":"1998","journal-title":"Oncogene"},{"key":"ref_42","first-page":"2705","article-title":"Tumor cell senescence in cancer treatment","volume":"63","author":"Roninson","year":"2003","journal-title":"Cancer Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/01635580903191585","article-title":"Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention","volume":"62","author":"Huang","year":"2010","journal-title":"Nutr. Cancer"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.foodchem.2010.08.012","article-title":"The molecular basis of working mechanism of natural polyphenolic antioxidants","volume":"125","author":"Leopoldini","year":"2011","journal-title":"Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"282","DOI":"10.2164\/jandrol.106.000968","article-title":"Mechanisms involved in resveratrol-induced apoptosis and cell cycle arrest in prostate cancer-derived cell lines","volume":"28","author":"Benitez","year":"2007","journal-title":"J. Androl."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.2741\/2199","article-title":"Structure-proteasome-inhibitory activity relationships of dietary flavonoids in human cancer cells","volume":"12","author":"Chen","year":"2007","journal-title":"Front. Biosci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1207\/S15327914NC381_16","article-title":"Flavonoids suppress androgen-independent human prostate tumor proliferation","volume":"38","author":"Knowles","year":"2000","journal-title":"Nutr. Cancer"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4290","DOI":"10.1021\/jf0502698","article-title":"Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements","volume":"53","author":"Prior","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.2174\/0929867053764635","article-title":"Metals, toxicity and oxidative stress","volume":"12","author":"Valko","year":"2005","journal-title":"Curr. Med. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2872","DOI":"10.1016\/j.lfs.2005.11.004","article-title":"Structure-radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants","volume":"78","author":"Cai","year":"2006","journal-title":"Life Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.bmc.2009.11.015","article-title":"Reliability of bond dissociation enthalpy calculated by the PM6 method and experimental TEAC values in antiradical QSAR of flavonoids","volume":"18","year":"2010","journal-title":"Bioorg. Med. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.ifset.2009.07.002","article-title":"Antioxidant properties of resveratrol: A structure-activity insight","volume":"11","author":"Gulcin","year":"2010","journal-title":"Innov. Food Sci. Emerg."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1038\/nature08506","article-title":"Predicting new molecular targets for known drugs","volume":"462","author":"Keiser","year":"2009","journal-title":"Nature"},{"key":"ref_54","first-page":"848","article-title":"Correlation of DPPIV expression with clinicopathological features and prognosis in epithelial ovarian carcinoma","volume":"30","author":"Zhang","year":"2008","journal-title":"Zhonghua Zhong Liu Za Zhi"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1158\/1078-0432.CCR-09-1805","article-title":"Metformin: A therapeutic opportunity in breast cancer","volume":"16","year":"2010","journal-title":"Clin. Cancer Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ejphar.2010.11.005","article-title":"Metformin reduces cisplatin-mediated apoptotic death of cancer cells through AMPK-independent activation of Akt","volume":"651","author":"Vilimanovich","year":"2011","journal-title":"Eur. J. Pharmacol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.cell.2007.03.039","article-title":"Structural basis for the inhibition of tyrosine kinase activity of ZAP-70","volume":"129","author":"Deindl","year":"2007","journal-title":"Cell"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1002\/med.20060","article-title":"New insights on the anticancer properties of dietary polyphenols","volume":"26","author":"Fresco","year":"2006","journal-title":"Med. Res. Rev."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.pharmthera.2011.02.004","article-title":"Polyphenols as small molecular inhibitors of signaling cascades in carcinogenesis","volume":"130","author":"Kang","year":"2011","journal-title":"Pharmacol. Ther."},{"key":"ref_60","first-page":"113","article-title":"Experience in the use of Tebrophen for influenza prevention","volume":"6","author":"Levchenko","year":"1975","journal-title":"Zh. Mikrobiol. Epidemiol. Immunobiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0047-6374(99)00090-1","article-title":"Sudden senescence syndrome plays a major role in cell culture proliferation","volume":"112","author":"Rubelj","year":"2000","journal-title":"Mech. Ageing Dev."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s12672-010-0044-4","article-title":"Androgen action in prostate cancer","volume":"1","author":"Basu","year":"2010","journal-title":"Horm. Cancer"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"4897","DOI":"10.3390\/molecules16064897","article-title":"Synthesis and in vitro protein tyrosine kinase inhibitory activity of furan-2-yl(phenyl)methanone derivatives","volume":"16","author":"Zheng","year":"2011","journal-title":"Molecules"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1006\/jmbi.1996.0477","article-title":"A fast flexible docking method using an incremental construction algorithm","volume":"261","author":"Rarey","year":"1996","journal-title":"J. Mol. Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/S0022-2836(77)80200-3","article-title":"The Protein Data Bank: A computer-based archival file for macromolecular structures","volume":"112","author":"Bernstein","year":"1977","journal-title":"J. Mol. Biol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"3325","DOI":"10.1021\/jm9706776","article-title":"A scoring scheme for discriminating between drugs and nondrugs","volume":"41","author":"Sadowski","year":"1998","journal-title":"J. Med. Chem."},{"key":"ref_67","unstructured":"Frisch, M.J., Trucks, G.W., Schlegel, H.B., Scuseria, G.E., Robb, M.A., Cheeseman, J.R., Montgomery, J.A., Vreven, T., Kudin, K.N., and Burant, J.C. (Gaussian 03, 2004). Gaussian 03."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1042\/bj3020737","article-title":"Rapid and efficient purification of Src homology 2 domain-containing proteins: Fyn, Csk and phosphatidylinositol 3-kinase p85","volume":"302","author":"Koegl","year":"1994","journal-title":"Biochem. J."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"6254","DOI":"10.1073\/pnas.88.14.6254","article-title":"Purification and initial characterization of the lymphoid-cell protein-tyrosine kinase p56lck from a baculovirus expression system","volume":"88","author":"Ramer","year":"1991","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/S0021-9258(18)48369-X","article-title":"Purification and initial characterization of the lymphocyte-specific protein-tyrosyl kinase p56lck from a baculovirus expression system","volume":"267","author":"Watts","year":"1992","journal-title":"J. Biol. Chem."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1093\/clinchem\/28.12.2423","article-title":"Optimizing the o-phenylenediamine assay for horseradish peroxidase: Effects of phosphate and pH, substrate and enzyme concentrations, and stopping reagents","volume":"28","author":"Bovaird","year":"1982","journal-title":"Clin. Chem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1023\/A:1016239528257","article-title":"A single-stage mechanism controls replicative senescence through Sudden Senescence Syndrome","volume":"3","author":"Rubelj","year":"2002","journal-title":"Biogerontology"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/17\/7\/7864\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:51:01Z","timestamp":1760219461000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/17\/7\/7864"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,28]]},"references-count":72,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2012,7]]}},"alternative-id":["molecules17077864"],"URL":"https:\/\/doi.org\/10.3390\/molecules17077864","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2012,6,28]]}}}