{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T18:01:02Z","timestamp":1792087262965,"version":"4.4.0"},"reference-count":26,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2012,8,29]],"date-time":"2012-08-29T00:00:00Z","timestamp":1346198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Antibacterial activity of berberine (Ber) and 8-acetonyl-dihydroberberine (A-Ber) alone and combined uses with antibacterial agents ampicillin (AMP), azithromycin (AZM), cefazolin (CFZ) and levofloxacin (LEV) was studied on 10 clinical isolates of SCCmec III type methicillin-resistant Staphylococcus aureus (MRSA). Susceptibility to each agent alone was tested using a broth microdilution method and the chequerboard and time-kill tests for the combined evaluations, respectively. The alone MICs\/MBCs (mg\/mL) ranges were 32\u2013128\/64\u2013256 (Ber) and 32-128\/128-512 (A-Ber). Significant synergies were observed for the Ber (A-Ber)\/AZM and Ber (A-Ber)\/LEV combinations against 90% of the tested MRSA strains, with fractional inhibitory concentration indices (FICIs) values ranged from 0.188 to 0.500. An additivity result was also observed for the Ber\/AZM combination by time-kill curves. These results demonstrated for the first time that Ber and A-Ber enhanced the in vitro inhibitory efficacy of AZM and LEV to a same extent, which had potential for further investigation in combinatory therapeutic applications of patients infected with MRSA.<\/jats:p>","DOI":"10.3390\/molecules170910322","type":"journal-article","created":{"date-parts":[[2012,8,29]],"date-time":"2012-08-29T11:47:52Z","timestamp":1346240872000},"page":"10322-10330","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Antibacterial and Synergy of Berberines with Antibacterial Agents against Clinical Multi-Drug Resistant Isolates of Methicillin-Resistant Staphylococcus aureus (MRSA)"],"prefix":"10.3390","volume":"17","author":[{"given":"Guo-Ying","family":"Zuo","sequence":"first","affiliation":[{"name":"Research Center for Natural Medicines, Kunming General Hospital, PLA, Kunming 650032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Research Center for Natural Medicines, Kunming General Hospital, PLA, Kunming 650032, China"},{"name":"Kunming Medical College, Kunming 650032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Han","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Yunnan Traditional Chinese Medical College, Kunming 650500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gen-Chun","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Center for Natural Medicines, Kunming General Hospital, PLA, Kunming 650032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Center for Natural Medicines, Kunming General Hospital, PLA, Kunming 650032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong-Qi","family":"Bian","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases, Kunming General Hospital, PLA, Kunming 650032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2012,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1136\/bmj.1.5219.124-a","article-title":"\u201cCelbenin\u201d-resistant staphylococci","volume":"124","author":"Jevons","year":"1961","journal-title":"Br. 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