{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T03:49:30Z","timestamp":1778989770276,"version":"3.51.4"},"reference-count":43,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2013,5,24]],"date-time":"2013-05-24T00:00:00Z","timestamp":1369353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Calophyllum brasiliense is a rich source of bioactive coumarins, xanthones and biflavonoids. The aim of the study was to compare the phenol contents and the antioxidant activity of C. brasiliense extracts obtained by conventional and supercritical fluid extraction (SFE) methods, as well as the quantification of crude extracts and (\u2212)-mammea A\/BB yields. Dichloromethane and hexane were used as solvents for the conventional extractions and SFE was developed using supercritical CO2; the kinetic curves were modeled using a second-order empirical model. The dichloromethane extract presented the best total yield, although it showed the lowest content of (\u2212)-mammea A\/BB. The concentration of the coumarin was considerably higher in extracts obtained by the supercritical fluid method and a higher antioxidant activity was assigned to extracts obtained by this technique. Concerning the total phenolic contents, both the dichloro-methane and the supercritical extractions produced satisfactory amounts. The SFE method proved to be more promising than conventional methods.<\/jats:p>","DOI":"10.3390\/molecules18066215","type":"journal-article","created":{"date-parts":[[2013,5,24]],"date-time":"2013-05-24T13:02:03Z","timestamp":1369400523000},"page":"6215-6229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Comparing Conventional and Supercritical Extraction of (\u2212)-Mammea A\/BB and the Antioxidant Activity of Calophyllum brasiliense Extracts"],"prefix":"10.3390","volume":"18","author":[{"given":"Renata","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Engenharia Qu\u00edmica, Universidade Estadual de Maring\u00e1 (UEM), Av. Colombo, 5790, Bloco E-46, 87020-900 Maring\u00e1, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Lemos","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Engenharia Qu\u00edmica, Universidade Estadual de Maring\u00e1 (UEM), Av. Colombo, 5790, Bloco E-46, 87020-900 Maring\u00e1, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6917-9323","authenticated-orcid":false,"given":"Ivana","family":"Leal","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Departamento de Produtos Naturais e Alimentos, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Bloco A, 2\u00b0andar, Ilha do Fund\u00e3o, Cidade Universit\u00e1ria, 21941-902, RJ, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celso","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Av. Colombo, 5790, Bloco K-68, 87020-900 Maring\u00e1, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di\u00f3genes","family":"Cortez","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Av. Colombo, 5790, Bloco K-68, 87020-900 Maring\u00e1, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edson","family":"Da Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Engenharia Qu\u00edmica, Universidade Estadual do Oeste do Paran\u00e1 (UNIOESTE), Caixa postal 520, 85903-000,Toledo, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Cabral","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Engenharia Qu\u00edmica, Universidade Estadual de Maring\u00e1 (UEM), Av. Colombo, 5790, Bloco E-46, 87020-900 Maring\u00e1, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00facio","family":"Cardozo-Filho","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Engenharia Qu\u00edmica, Universidade Estadual de Maring\u00e1 (UEM), Av. Colombo, 5790, Bloco E-46, 87020-900 Maring\u00e1, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.5962\/bhl.part.8541","article-title":"A revision of the Old World species of Calophyllum (Guttiferae)","volume":"61","author":"Stevens","year":"1980","journal-title":"J. Arnold Arbor."},{"key":"ref_2","unstructured":"Cronquist, A. (1981). An Integrated System of Classification of Flowering Plants, Columbia University Press."},{"key":"ref_3","unstructured":"Corr\u00eaa, M.P. (1978). Dicion\u00e1rio das plantas \u00fateis do Brasil e das ex\u00f3ticas cultivadas, Imprensa Nacional."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1023\/B:JOEC.0000006459.88330.61","article-title":"Antifungal Xanthones from Calophyllum brasiliensis Heartwood.","volume":"23","year":"1997","journal-title":"J. Chem. Ecol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1021\/np980140a","article-title":"Pyranocoumarins from Tropical species of the genus Calophyllum: A chemotaxonomic study of extracts in the Natural Cancer Institute Collection","volume":"61","author":"Mckee","year":"1998","journal-title":"J. Nat. Prod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1016\/S0031-9422(98)00480-4","article-title":"Epimeers from the leaves of Calophylluminophyllum","volume":"50","author":"Ali","year":"1999","journal-title":"Phytochemistry"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1021\/np000215m","article-title":"New xanthones from Calophyllumcaledonicum","volume":"63","author":"Morel","year":"2000","journal-title":"J. Nat. Prod."},{"key":"ref_8","first-page":"431","article-title":"Chemical composition and analgesic activity of Calophyllumbrasiliense.","volume":"56","author":"Silva","year":"2001","journal-title":"Therapie"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0378-8741(98)00244-X","article-title":"Gastroprotective effect from Calophyllumbrasiliense Camb. bark on experimental gastric lesions in rats and mice","volume":"67","author":"Sartori","year":"1999","journal-title":"J. Ethnopharmacol."},{"key":"ref_10","unstructured":"Lewis, W.H. (1977). Medical Botany \u2013 Plants Affecting Man\u2019s Health, John Wiley & Sons."},{"key":"ref_11","unstructured":"Duke, J.A., and Martinez, R.V. (1994). Amazonian Ethnobotanical Dictionary, CRC Press."},{"key":"ref_12","unstructured":"Vasquez, M.R. (1990). Useful plants of Amazonian Peru, Filled with USDA\u2019s National Agricultural Library. Second Draft."},{"key":"ref_13","unstructured":"Rutter, R.A. (1990). Catalogo de plantas utiles de Ia Amazonia Peruana, Instituto Ling\u00fc\u00edstico de Verano."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1021\/np030438n","article-title":"New Chromanone acids with Antibacterial activity from Calophyllum brasiliense","volume":"67","author":"Cottiglia","year":"2004","journal-title":"J. Nat. Prod."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1515\/znc-2004-9-1009","article-title":"Antimicrobial Activity of Fractions and Compounds from Calophyllum brasiliense (Clusiaceae\/Guttiferae)","volume":"59","author":"Pretto","year":"2004","journal-title":"Z. Naturforsch."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1016\/j.lfs.2004.03.017","article-title":"Cytotoxic effects of mammea type coumarins from Calophyllum brasiliense","volume":"75","author":"Apan","year":"2004","journal-title":"Life Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1021\/np0203640","article-title":"Structure of three new coumarins and cancer chemopreventive activity of 4- substituted coumarins","volume":"66","author":"Ito","year":"2003","journal-title":"J. Nat. Prod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1248\/bpb.27.1471","article-title":"HIV-1 inhibitory compounds from Calophyllumbrasiliense leaves","volume":"27","author":"Basualdo","year":"2004","journal-title":"Biol. Pharm. Bull."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1007\/s00436-007-0542-7","article-title":"Antileishmanial activity of crude extract and coumarin from Calophyllumbrasiliensis leaves against Leishmaniaamazonensis","volume":"101","author":"Brenzan","year":"2007","journal-title":"Parasitol. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.phymed.2009.08.011","article-title":"Efficacy of components from leaves of Calophyllumbrasiliense against Leishmania (Leishmania) amazonensis","volume":"17","author":"Honda","year":"2010","journal-title":"Phytomedicine"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1021\/np010398s","article-title":"Chemical constituents of Calophyllumbrasiliensis: Structure elucidation of seven new xanthones and their cancer chemopreventive activity","volume":"65","author":"Ito","year":"2002","journal-title":"J. Nat. Prod."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.supflu.2005.01.003","article-title":"Supercritical fluid extraction of antioxidant compounds from oregano","volume":"38","author":"Cavero","year":"2006","journal-title":"J. Supercrit. Fluids"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.cej.2009.08.016","article-title":"Response surface method applied to supercritical carbon dioxide extraction of Vetiveriazizanioides essential oil","volume":"155","author":"Danh","year":"2009","journal-title":"Chem. Eng. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.cej.2007.06.027","article-title":"Extraction of natural compounds with biological activity from sunflower leaves using supercritical carbon dioxide","volume":"152","author":"Casas","year":"2009","journal-title":"Chem. Eng. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.cej.2008.12.006","article-title":"Kinetics of the supercritical fluid extraction of carotenoids from microalgae with CO2 and ethanol as cosolvent","volume":"150","author":"Serrano","year":"2009","journal-title":"Chem. Eng. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/S0021-9673(98)00906-6","article-title":"Supercritical fluid extraction of flavonoids from Scutellariae Radix","volume":"830","author":"Lin","year":"1999","journal-title":"J. Chrom. A"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Noldin, V.F., Isaias, D.B., and Cechinel-Filho, V. G\u00eanero Calophyllum: import\u00e2ncia qu\u00edmica e farmacol\u00f3gica. Qu\u00edm. Nova., 29, 549\u2013554.","DOI":"10.1590\/S0100-40422006000300025"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1211\/jpp.59.5.0013","article-title":"Antiproliferative, cytotoxic and antitumour activity of coumarins isolated from Calophyllumbrasiliense","volume":"59","author":"Estrada","year":"2007","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1590\/S0100-40422005000400003","article-title":"Estudo fitoqu\u00edmico e avalia\u00e7\u00e3o da atividade moluscicida do Calophyllum brasiliense CAMB (CLUSIACEAE)","volume":"28","author":"Brenzan","year":"2005","journal-title":"Quim. Nova"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1590\/S0074-02762008000500004","article-title":"Trypanocidal constituents in plants: 7. Mammea-type coumarins","volume":"103","author":"Abe","year":"2008","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.supflu.2010.08.015","article-title":"Bioactive extracts of orange (Citrus sinensis L. Osbeck) pomace obtained by SFE and low pressure techniques: Mathematical modeling and extract composition","volume":"55","author":"Benellia","year":"2010","journal-title":"J. Supercrit. Fluids"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2558","DOI":"10.1021\/jf0732096","article-title":"Extraction of grape seed oil using compressed carbon dioxide and propane: Extraction yields and characterization of free glycerol compounds","volume":"56","author":"Oliveira","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3042","DOI":"10.1021\/jf030575q","article-title":"Chemical composition and extraction yield of the extract of Origanumvulgare obtained from sub and supercritical CO2","volume":"52","author":"Rodrigues","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"25","DOI":"10.17348\/era.3.0.25-36","article-title":"Antioxidant potential of seven myrtaceous fruits","volume":"3","author":"Reynertson","year":"2005","journal-title":"Ethnobot. Res. Applic."},{"key":"ref_35","unstructured":"(2000). Normas Anal\u00edticas do Instituto Adolfo Lutz: M\u00e9todos qu\u00edmicos e f\u00edsicos para an\u00e1lise de alimentos, IAL."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1021\/jf052858j","article-title":"Supercritical CO2 Extraction and Purification of Compounds with Antioxidant Activity","volume":"54","author":"Moure","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0896-8446(02)00013-X","article-title":"Extraction of ginger (Zingiberofficinale Roscoe) oleoresin with CO2 and cosolvents: A study of the antioxidant action of the extracts.","volume":"24","author":"Zancan","year":"2002","journal-title":"J. Supercrit. Fluids"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1080\/10826070903442444","article-title":"Quantitative and qualitative analysis of (-) mammea a\/bb coumarin in extracts of Calophyllumbrasiliense Cambess (clusiaceae) by HPLC","volume":"33","author":"Brenzan","year":"2010","journal-title":"J. Liq. Chrom. Relat. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.foodchem.2004.10.006","article-title":"Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activit","volume":"91","author":"Meda","year":"2005","journal-title":"Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1038\/1811199a0","article-title":"Antioxidant determinations by the use of a stable free radical","volume":"18","author":"Blois","year":"1958","journal-title":"Nature"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0023-6438(95)80008-5","article-title":"Use of a free radical method to evaluate antioxidant activity","volume":"28","author":"Cuvelier","year":"1995","journal-title":"Lebensm.-Wiss. u.-Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.supflu.2009.11.012","article-title":"Extraction of sesame seed (Sesamunindicum L.) oil using compressed propane and supercritical carbon dioxide","volume":"52","author":"Corso","year":"2010","journal-title":"J. Supercrit. Fluid."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.supflu.2008.08.001","article-title":"Supercritical extraction process and phase equilibrium of Candeia (Eremanthuserythropappus) oil using supercritical carbon","volume":"47","author":"Benazzi","year":"2008","journal-title":"J. Supercrit. Fluid."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/18\/6\/6215\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:46:57Z","timestamp":1760219217000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/18\/6\/6215"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,24]]},"references-count":43,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2013,6]]}},"alternative-id":["molecules18066215"],"URL":"https:\/\/doi.org\/10.3390\/molecules18066215","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,24]]}}}