{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T16:00:36Z","timestamp":1780588836436,"version":"3.54.1"},"reference-count":46,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2013,7,18]],"date-time":"2013-07-18T00:00:00Z","timestamp":1374105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A methanolic crude extract of Parkia biglobosa was prepared and later partitioned in succession with different solvents of increasing polarity ranging from  n-hexane, chloroform and ethyl acetate to butanol. Phytochemical screening of the extract revealed the presence of alkaloids, tannins, saponins, flavonoids, steroids, glycoside and sugars. The inhibition zones exhibited by the extract against the tested bacteria ranged between 14 \u00b1 0.00 mm (against Escherichia coli) and 28 \u00b1 0.71 mm (against Pseudomonas aeruginosa). The MIC of the methanolic extract of P. biglobosa against isolates ranged between 0.63 mg\/mL and 5 mg\/mL, while the MIC values exhibited by the n-hexane and aqueous fractions ranged between 0.63 mg\/mL and 10 mg\/mL. Overall the extract and fractions of P. biglobosa used in this work were found to possess antimicrobial properties which compared favourably with those of streptomycin. These observations make this plant a potential source of bioactive compounds that can be used in management of bacterial infections. The use of this plant as herbal medicaments in African countries and the reports on the toxicity of the plant further show that the plant is non-toxic to humans.<\/jats:p>","DOI":"10.3390\/molecules18078485","type":"journal-article","created":{"date-parts":[[2013,7,18]],"date-time":"2013-07-18T11:20:08Z","timestamp":1374146408000},"page":"8485-8499","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Preliminary Phytochemical Screening and Antibacterial Properties of Crude Stem Bark Extracts and Fractions of  Parkia biglobosa (Jacq.)"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2100-3422","authenticated-orcid":false,"given":"Emmanuel","family":"Abioye","sequence":"first","affiliation":[{"name":"Department of Microbiology, Obafemi Awolowo University, Ile-Ife 234, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Akinpelu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Obafemi Awolowo University, Ile-Ife 234, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olayinka","family":"Aiyegoro","sequence":"additional","affiliation":[{"name":"GI Microbiology and Biotechnology Unit, Agricultural Research Council,  Animal Production Institute, Irene 0062, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mobolaji","family":"Adegboye","sequence":"additional","affiliation":[{"name":"Department of Microbiology, North West University, Mafikeng Campus, Mmabatho 2745,  South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Oni","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Oduduwa University, Ipetumodu 234, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9770-085X","authenticated-orcid":false,"given":"Anthony","family":"Okoh","sequence":"additional","affiliation":[{"name":"Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2013,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S122","DOI":"10.1038\/nm1145","article-title":"Antibacterial resistance worldwide: Causes, challenges and responses","volume":"10","author":"Levy","year":"2004","journal-title":"Nat. Med."},{"key":"ref_2","first-page":"49","article-title":"Antibacterial activities and bioactive components of the aqueous fraction of the stem bark of Parkia biglobosa (Jacq) (Mimosaceae)","volume":"7","author":"Udobi","year":"2008","journal-title":"Nigerian J. Pharm. Sc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1046\/j.1472-765X.2001.00923.x","article-title":"Screening of some plants from Northern Argentina for their antimicrobial activity","volume":"32","author":"Salvat","year":"2001","journal-title":"Lett. Applied Microbiol."},{"key":"ref_4","first-page":"839","article-title":"Antimicrobial activity of some important medical plant against plant and human pathogen","volume":"4","author":"Mahesh","year":"2008","journal-title":"World J. Agric. Sc."},{"key":"ref_5","first-page":"104","article-title":"Techniques for evaluation of medicinal plant products as antimicrobial agent: current methods and future trends","volume":"4","author":"Das","year":"2010","journal-title":"J. Med. Plt. Res."},{"key":"ref_6","first-page":"247","article-title":"Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria","volume":"31","author":"Gislene","year":"2000","journal-title":"Brazilian J. Microbiol."},{"key":"ref_7","first-page":"1272","article-title":"In vitro antibacterial activity of Parkia biglobosa (Jacq.) root bark extract against some microorganisms associated with urinary tract infections","volume":"6","author":"Ameh","year":"2007","journal-title":"Afr. J. Biotech."},{"key":"ref_8","first-page":"596","article-title":"Antibacterial potential of the methanolic extract and aqueous fraction of the stem bark of the African locust bean (Parkia biglobosa)","volume":"43","author":"Udobi","year":"2010","journal-title":"Eur. J.Sci. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1080\/01435698.1992.9752911","article-title":"Parkia biglobosa: A potential multipurpose fodder tree legume in West Africa","volume":"7","author":"Sabiti","year":"1992","journal-title":"Int. Tr. Cr. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1095-8339.1983.tb00987.x","article-title":"The taxonomy, reproductive biology and economic potential of Parkia (Leguminosae: Mimosideae) in Africa and Madagascar","volume":"87","author":"Hopkins","year":"1983","journal-title":"Bot. J. Lin. Soc."},{"key":"ref_11","first-page":"125","article-title":"Phytochemical and antibacterial properties of Parkia biglobosa and Parkia bicolor leaf extracts","volume":"5","author":"Ajaiyeoba","year":"2002","journal-title":"Afr. J. Biomed. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1002\/1099-1573(200012)14:8<635::AID-PTR427>3.0.CO;2-T","article-title":"Analgesic and anti-inflammatory activities of an extract from Parkia biglobosa used in traditional medicine in the Ivory Coast","volume":"14","author":"Kouadio","year":"2000","journal-title":"Phyto. Res."},{"key":"ref_13","first-page":"392","article-title":"Antimicrobial effects of the stem Bark extracts of Parkia biglobosa (Jacq.) on Shigellae","volume":"4","author":"Guissou","year":"2007","journal-title":"Afr. J. Trad. Compl. Alt. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1111\/j.1440-1681.2006.04444.x","article-title":"Possible antidiabetic and antihyperlipidaemic effect of fermented Parkia biglobosa (JACQ) extract in alloxan-induced diabetic rats","volume":"33","author":"Odetola","year":"2006","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_15","first-page":"338","article-title":"Phytochemical analysis and antibacterial evaluation of the leaf stem bark and root of the African locust bean (Parkia biglobosa)","volume":"3","author":"Udobi","year":"2009","journal-title":"J. Med. Pl. Res."},{"key":"ref_16","unstructured":"Agro Forestry Database: A tree species reference and selection guide. Available online:http:\/\/www.worldagroforestrycentre.org\/sea\/products\/afdbases\/af\/asp\/SpeciesInfo.asp?SpID=1255."},{"key":"ref_17","first-page":"8","article-title":"Antiplasmodial activities of Parkia biglobosa leaves: In vivo and in vitro studies","volume":"2","author":"Builders","year":"2011","journal-title":"An. Biol. Res."},{"key":"ref_18","first-page":"238","article-title":"Comparative Study of Leaf and Stem Bark Extracts of Parkia biglobosa against Enterobacteria","volume":"5","author":"Guissou","year":"2008","journal-title":"Afr. J. Trad. Com."},{"key":"ref_19","first-page":"191","article-title":"Toxicity and Population supressions effects (Parkia clappertonium) and dried fish pests (Dermestes maculated) and Necrobia rufipes","volume":"6","author":"Odeyemi","year":"2000","journal-title":"Global J. Pure Appl. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0027-5107(01)00073-2","article-title":"Role of plant polyphenols in genomic stability","volume":"475","author":"Ferguson","year":"2001","journal-title":"Mut. Res. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0378-8741(96)85514-0","article-title":"Comparative study on the antibacterial activity of phytochemical flavanones against methicillin-resistant Staphylococcus aureus","volume":"50","author":"Tsuchiya","year":"1996","journal-title":"J. Ethnopharmacol."},{"key":"ref_22","first-page":"30","article-title":"Antimicrobial properties of stem bark extract of Ximenia americana","volume":"1","author":"Maikai","year":"2009","journal-title":"J. Agric. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3875","DOI":"10.1016\/0031-9422(91)83426-L","article-title":"Antimicrobial properties of tannins","volume":"30","author":"Scalbert","year":"1991","journal-title":"Phytochemistry"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1093\/jac\/48.4.487","article-title":"Antibacterial action of several tannins against Staphylococcus aureus","volume":"48","author":"Hisanori","year":"2001","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_25","unstructured":"Dharmananda, S. (,  2003). Gallnuts and the uses of tannins in Chinese medicine. Proceedings of institute for Traditional Medicine, Portland, OR, USA."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.2174\/0929867023369709","article-title":"Traditional Chinese medicine (TCM): Are polyphenols and saponins the key ingredients triggering biological activities?","volume":"9","author":"Liu","year":"2002","journal-title":"Cur. Med. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1055\/s-2006-957469","article-title":"Anti-inflammatory activity of unusual lupine saponins from Bupleurum fruticescens","volume":"64","author":"Just","year":"1998","journal-title":"Pl. Med. J."},{"key":"ref_28","first-page":"116","article-title":"Leaf and seed extracts of Bixa orellana L. exerts anti-microbial activity against bacterial pathogens","volume":"1","author":"Tamil","year":"2011","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1038\/368753a0","article-title":"Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancers","volume":"368","author":"Nobori","year":"1994","journal-title":"Nature"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1078\/0944-7113-00055","article-title":"Inhibition of cellular immune responses by Tylophoraindica in experimental models","volume":"8","author":"Ganguly","year":"2001","journal-title":"Phytomedicine"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1021\/np0003443","article-title":"Cytotoxic activity of some phenanthroindolizidine N-oxide alkaloids from Cynanchum vincetoxicum","volume":"63","author":"Staerk","year":"2000","journal-title":"J. Nat.Prod."},{"key":"ref_32","first-page":"131","article-title":"New leads to the treatment of protozoal infections based on natural product molecules","volume":"1","author":"Phillipson","year":"1987","journal-title":"Acta Pharm. Nord."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1021\/np50120a012","article-title":"Quantitative HPLC analysis of cardiac glycosides in Digitalis purpurea leaves","volume":"58","author":"Ikeda","year":"1995","journal-title":"J. Nat. Prod."},{"key":"ref_34","first-page":"160","article-title":"Evaluation of the chemical composition of medicinal plants belonging to Euphorbiaceae in Pakistan","volume":"14","author":"Okwu","year":"2001","journal-title":"Pakistan Vet. J."},{"key":"ref_35","first-page":"518","article-title":"A randomized placebo-controlled trial of the efficacy of beta-sitosterol and its glucoside as adjuvants in the treatment of pulmonary tuberculosis","volume":"1","author":"Donald","year":"1997","journal-title":"Int. J. Tuber Lung Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1016\/S0140-6736(95)91085-9","article-title":"The B-sitosterol study group: randomized, placebo-controlled, double-blind clinical trial of B-sitosterol in patients with benign prostatic hyperplasia","volume":"345","author":"Berges","year":"1995","journal-title":"Lancet"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1093\/jac\/dki158","article-title":"Risk factors for antibiotic resistant Escherichia coli isolated from community-acquired urinary tract infections in Dakar, Senegal","volume":"56","author":"Dromigny","year":"2005","journal-title":"J. Antimicrob. Chemoth."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1093\/infdis\/153.6.1075","article-title":"High-level resistance to gentamicin in Streptococcus faecalis: risk factors and evidence for exogenous acquisition of infection","volume":"153","author":"Zervos","year":"1986","journal-title":"J. Infect. Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1998","DOI":"10.1128\/IAI.01232-10","article-title":"Involvement of epidermal growth factor receptor-linked signaling responses in Pseudomonas fluorescens infected alveolar epithelial cells","volume":"79","author":"Hye","year":"2011","journal-title":"Infect. Immunity."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1086\/592968","article-title":"Multistate outbreak of Pseudomonas fluorescens blood stream infection after exposure to contaminated heparinized saline flush prepared by a compounding pharmacy","volume":"47","author":"Gershman","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"ref_41","first-page":"102","article-title":"In vitro antibacterial activities of extracts of Nauclea latifolia and Daniella oliveri","volume":"3","author":"Doughari","year":"2008","journal-title":"Sci. Res. Essay"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"685","DOI":"10.5897\/AJB2005.000-3127","article-title":"Phytochemical constituent of some nigerian medicinal plants","volume":"4","author":"Edeoga","year":"2005","journal-title":"Afr. J. Biotechnol."},{"key":"ref_43","unstructured":"Trease, G.E., and Evans, W.C. (1983). Pharmacognosy, Bailliers Tindal. [12th ed.]."},{"key":"ref_44","unstructured":"Harbourne, J.B. (1983). Chapman and Hall."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1590\/S0074-02762006000400007","article-title":"Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases","volume":"101","author":"Betoni","year":"2006","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_46","first-page":"589","article-title":"Chemical analyses of aqueous extract of Parkia biglobosa fruit husk collected from Northern Ghana","volume":"8","author":"Abagale","year":"2013","journal-title":"Sc. Res. Ess."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/18\/7\/8485\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:48:04Z","timestamp":1760219284000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/18\/7\/8485"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,18]]},"references-count":46,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2013,7]]}},"alternative-id":["molecules18078485"],"URL":"https:\/\/doi.org\/10.3390\/molecules18078485","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,18]]}}}