{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T22:55:52Z","timestamp":1773183352888,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2013,8,16]],"date-time":"2013-08-16T00:00:00Z","timestamp":1376611200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Several azide-derivatized and fluorescently-labeled peptides were immobilized on azadibenzocyclooctyne (ADIBO)-activated slide surfaces via a strain-promoted  alkyne-azide cycloaddition (SPAAC) reaction revealing excellent immobilization kinetics, good spot homogeneities and reproducible fluorescence signal intensities. A myc-peptide micro-array immunoassay showed an antibody limit-of-detection (LOD) superior to a microtiter plate-based ELISA. Bovine serum albumin (BSA) and dextran covalently attached via \u201cclick\u201d chemistry more efficiently reduced non-specific binding (NSB) of fluorescently-labeled IgG to the microarray surface in comparison to immobilized hexanoic acid and various types of polyethylene glycol (PEG) derivatives. Confirmation of these findings via further studies with other proteins and serum components could open up new possibilities for human sample and microarray platform-based molecular diagnostic tests.<\/jats:p>","DOI":"10.3390\/molecules18089833","type":"journal-article","created":{"date-parts":[[2013,8,16]],"date-time":"2013-08-16T12:14:49Z","timestamp":1376655289000},"page":"9833-9849","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["ADIBO-Based \u201cClick\u201d Chemistry for Diagnostic Peptide  Micro-Array Fabrication: Physicochemical and  Assay Characteristics"],"prefix":"10.3390","volume":"18","author":[{"given":"Denis","family":"Prim","sequence":"first","affiliation":[{"name":"Institute of Life Technologies, University of Applied Sciences Western Switzerland, Sion 1950, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabien","family":"Rebeaud","sequence":"additional","affiliation":[{"name":"Institute of Life Technologies, University of Applied Sciences Western Switzerland, Sion 1950, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Cosandey","sequence":"additional","affiliation":[{"name":"Institute of Life Technologies, University of Applied Sciences Western Switzerland, Sion 1950, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Marti","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, University of Applied Sciences Western Switzerland, Fribourg 1705, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9593-410X","authenticated-orcid":false,"given":"Philippe","family":"Passeraub","sequence":"additional","affiliation":[{"name":"Institute of Sciences and Industrial Technologies, University of Applied Sciences Western Switzerland, Geneva 1202, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Pfeifer","sequence":"additional","affiliation":[{"name":"Institute of Life Technologies, University of Applied Sciences Western Switzerland, Sion 1950, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"365","DOI":"10.2174\/138620706777452429","article-title":"Microarray technology as a universal tool for high-throughput analysis of biological systems","volume":"9","author":"Sobek","year":"2006","journal-title":"Comb. 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