{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:34:11Z","timestamp":1760243651569,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T00:00:00Z","timestamp":1381881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>High-speed counter-current chromatography (HSCCC) was applied for the first time for the preparative separation of spirobisnaphthalenes from a crude extract of the endophytic fungus Berkleasmium sp. Dzf12, associated with the medicinal plant Dioscorea zingiberensis. Six spirobisnaphthalenes were successfully separated by HSCCC with a  two-phase solvent system composed of n-hexane-chloroform-methanol-water (1.5:3.0:2.5:2.0, v\/v). About 18.0 mg of diepoxin k (1), 245.7 mg of palmarumycin C13 (2), 42.4 mg of palmarumycin C16 (3), 42.2 mg of palmarumycin C15 (4), 32.6 mg of diepoxin \u03b4 (5), and 22.3 mg of diepoxin \u03b3 (6) with purities of 56.82, 71.39, 76.57, 75.86, 91.01 and 82.48%, respectively, as determined by high-performance liquid chromatography (HPLC), were obtained from 500 mg of the crude extract in a one-step elution within 7 h of separation procedure by HSCCC. The purified spirobisnaphthalenes were further structurally characterized by means of physicochemical and spectrometric analysis.<\/jats:p>","DOI":"10.3390\/molecules181012896","type":"journal-article","created":{"date-parts":[[2013,10,16]],"date-time":"2013-10-16T12:20:47Z","timestamp":1381926047000},"page":"12896-12908","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Preparative Separation of Spirobisnaphthalenes from Endophytic Fungus Berkleasmium sp. Dzf12 by  High-Speed Counter-Current Chromatography"],"prefix":"10.3390","volume":"18","author":[{"given":"Tijiang","family":"Shan","sequence":"first","affiliation":[{"name":"MOA Key Laboratory of Plant Pathology, Department of Plant Pathology,  College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiqiong","family":"Lu","sequence":"additional","affiliation":[{"name":"MOA Key Laboratory of Plant Pathology, Department of Plant Pathology,  College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Luo","sequence":"additional","affiliation":[{"name":"MOA Key Laboratory of Plant Pathology, Department of Plant Pathology,  College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiya","family":"Luo","sequence":"additional","affiliation":[{"name":"MOA Key Laboratory of Plant Pathology, Department of Plant Pathology,  College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Mou","sequence":"additional","affiliation":[{"name":"MOA Key Laboratory of Plant Pathology, Department of Plant Pathology,  College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry, College of Science, China Agricultural University,  Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youliang","family":"Peng","sequence":"additional","affiliation":[{"name":"MOA Key Laboratory of Plant Pathology, Department of Plant Pathology,  College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4681-191X","authenticated-orcid":false,"given":"Ligang","family":"Zhou","sequence":"additional","affiliation":[{"name":"MOA Key Laboratory of Plant Pathology, Department of Plant Pathology,  College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,10,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1039\/c0np00031k","article-title":"Structure, bioactivities, biosynthetic relationships and chemical synthesis of the spirodioxynaphthalenes","volume":"27","author":"Cai","year":"2010","journal-title":"Nat. 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