{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T23:54:25Z","timestamp":1776988465600,"version":"3.51.4"},"reference-count":32,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T00:00:00Z","timestamp":1386028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Astragali Radix (Huang-Qi) is a popular herbal medicine commonly used as a constituent in tonic herbal preparations. Hedysarum polybotrys Handel-Mazzetti is  one species used of Astragali Radix. In this study, the immunomodulatory properties of  H. polybotrys were explored by LPS-activated and SNP-treated RAW 264.7 cells and splenocytes and, daunoblastina-induced leucopenia BALB\/c mice. Formononetin was used as the bioactive marker to monitor the quality of the H. polybotrys extracts. H. polybotrys was extracted with hot-water and methanol, and MeOH extract partitioned with H2O  (M-H) and ethyl acetate (M-EA) to yield four different fractions. M-EA had the highest formononetin and total proanthocyanidin content and showed stronger inhibitory effects on the production and expression of NO, PGE2, iNOS and COX-2 in LPS-activated RAW 264.7 cells and splenocytes than the other fractions. In addition, M-EA significantly stimulated the proliferation of LPS-activated RAW 264.7 cells and splenocytes, enhanced NO radicals scavenging and attenuated NO-induced cytotoxicity.  Furthermore, M-EA also significantly increased the rate of recovery of white blood cells level in daunoblastina-induced leucopenia mice. These evidences suggest that this traditional Qi-tonifying herb has potential effects in clinical conditions when immune-enhancing and anti-inflammatory effect is desired.<\/jats:p>","DOI":"10.3390\/molecules181214862","type":"journal-article","created":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T11:15:30Z","timestamp":1386069330000},"page":"14862-14875","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Immunomodulatory Effects of Hedysarum polybotrys Extract in Mice Macrophages, Splenocytes and Leucopenia"],"prefix":"10.3390","volume":"18","author":[{"given":"Guan-Cheng","family":"Huang","sequence":"first","affiliation":[{"name":"Division of Hemato-Oncology, Department of Internal Medicine, Yuan's General Hospital, No.162 Cheng Kung 1st Road, Kaohsiung City 80249, Taiwan"},{"name":"Program of Health-Business Administration, School of Nursing, Fooyin University, No.151 Jinxue Road, Kaohsiung City 83102, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia-Jung","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Traditional Medicine, School of Medicine, National Yang-Ming University,  No.155 Section 2, Linong Street, Taipei City 11221, Taiwan"},{"name":"Department of Education and Research, Taipei City Hospital Renai Branch, No.10, Section 4,  Renai Road, Taipei City 10629, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun-Teng","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8994-4750","authenticated-orcid":false,"given":"Bor-Chun","family":"Weng","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, No.300 Syuefu Road, Chiayi City 60004, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting-Yi","family":"Chien","sequence":"additional","affiliation":[{"name":"School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung-Hui","family":"Tseng","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Medicine, Taipei Medical University, 250 Wu-Hsing Street,  Taipei City 11031, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching-Chiung","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,12,3]]},"reference":[{"key":"ref_1","unstructured":"Li, S. 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