{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T12:44:24Z","timestamp":1769431464745,"version":"3.49.0"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2014,4,2]],"date-time":"2014-04-02T00:00:00Z","timestamp":1396396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Laetia suaveolens, known as \u201ccasinga-cheirosa\u201d, crude extract EB719 has previously shown cytotoxic activity against prostate cancer and squamous cell carcinoma. For the first time, seven molecules were isolated from its apolar\u2014\u03b1-tocopherol (1) and sitosterol (2)\u2014and polar\u20143-O-caffeoylquinic acid (3), 4-O-caffeoylquinic acid (4),  5-O-feruloylquinic acid (5), hyperoside (6), and isoquercitrin (7)\u2014fractions. Acute toxicity was determined in a two-stage experiment: (1) a reduced number of Balb-c male mice received 5000 mg\/kg of EB719 to allow evaluation of general activity and other 27 parameters, plus death, up to the establishment of non-lethal dose (NLD), as well as lethal dose 50% (LD50); (2) NLD was administered and diazepam introduced as reference drug. EB719 showed LD50 = 178.0 mg\/kg, and NLD 156.3 mg\/kg. In stage one EB719 did not influence general activity, but provoked impairment in grasp reflexes, tail squeeze and breathing; piloerection and cyanosis were increased. In stage two, alterations occurred in auricular reflex, piloerection and breathing after diazepam administration, but not in response to EB719. Intestinal hemorrhage caused by local bleeding was observed after necropsy, and may be the main cause of animals\u2019 death other than a systemic effect of the extract. Although the isolated compounds are biologically and pharmacologically active in both men and animal systems, it is premature to relate their occurrence in EB719 to the observed intestine hemorrhage in mice.<\/jats:p>","DOI":"10.3390\/molecules19043973","type":"journal-article","created":{"date-parts":[[2014,4,2]],"date-time":"2014-04-02T11:09:47Z","timestamp":1396436987000},"page":"3973-3987","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["First Chemical Evaluation and Toxicity of Casinga-cheirosa to Balb-c Male Mice"],"prefix":"10.3390","volume":"19","author":[{"given":"Dirce","family":"Estork","sequence":"first","affiliation":[{"name":"Graduate Program in Veterinary, Graduate and Research Vice-Dean Office, Paulista University,  R. Dr. Bacelar, 1212, Vila Clementino, S\u00e3o Paulo 04026-002, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Gusm\u00e3o","sequence":"additional","affiliation":[{"name":"Graduate Program in Veterinary, Graduate and Research Vice-Dean Office, Paulista University,  R. Dr. Bacelar, 1212, Vila Clementino, S\u00e3o Paulo 04026-002, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mateus","family":"Paciencia","sequence":"additional","affiliation":[{"name":"Center for Research in Biodiversity, Botany Laboratory and Herbarium UNIP and Extraction Laboratory, Paulista University, Av. Paulista, 900, 1\u00b0 andar, Cerqueira C\u00e9sar, S\u00e3o Paulo 01310-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrit","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Center for Research in Biodiversity, Botany Laboratory and Herbarium UNIP and Extraction Laboratory, Paulista University, Av. Paulista, 900, 1\u00b0 andar, Cerqueira C\u00e9sar, S\u00e3o Paulo 01310-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Varella","sequence":"additional","affiliation":[{"name":"Center for Research in Biodiversity, Botany Laboratory and Herbarium UNIP and Extraction Laboratory, Paulista University, Av. Paulista, 900, 1\u00b0 andar, Cerqueira C\u00e9sar, S\u00e3o Paulo 01310-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riad","family":"Younes","sequence":"additional","affiliation":[{"name":"Center for Research in Biodiversity, Botany Laboratory and Herbarium UNIP and Extraction Laboratory, Paulista University, Av. Paulista, 900, 1\u00b0 andar, Cerqueira C\u00e9sar, S\u00e3o Paulo 01310-100, Brazil"},{"name":"Hospital S\u00e3o Jos\u00e9, R. Martiniano de Carvalho, 965, Bela Vista, 1321-001, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz","family":"Reis","sequence":"additional","affiliation":[{"name":"Education and Research Center, S\u00edrio Liban\u00eas Hospital, R. Adma Jafet, 91, Bela Vista,  S\u00e3o Paulo 01308-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edna","family":"Montero","sequence":"additional","affiliation":[{"name":"Medicine College, S\u00e3o Paulo University, LIM-62, Av. Dr. Arnaldo, 455, Pinheiros,  S\u00e3o Paulo 01246-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Bernardi","sequence":"additional","affiliation":[{"name":"Graduate Program in Veterinary, Graduate and Research Vice-Dean Office, Paulista University,  R. Dr. Bacelar, 1212, Vila Clementino, S\u00e3o Paulo 04026-002, Brazil"},{"name":"Graduate Program in Dentistry, Graduate and Research Vice-Dean Office, Paulista University,  R. Dr. Bacelar, 1212, Vila Clementino, S\u00e3o Paulo 04026-002, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Suffredini","sequence":"additional","affiliation":[{"name":"Graduate Program in Veterinary, Graduate and Research Vice-Dean Office, Paulista University,  R. Dr. Bacelar, 1212, Vila Clementino, S\u00e3o Paulo 04026-002, Brazil"},{"name":"Center for Research in Biodiversity, Botany Laboratory and Herbarium UNIP and Extraction Laboratory, Paulista University, Av. Paulista, 900, 1\u00b0 andar, Cerqueira C\u00e9sar, S\u00e3o Paulo 01310-100, Brazil"},{"name":"Graduate Program in Dentistry, Graduate and Research Vice-Dean Office, Paulista University,  R. Dr. Bacelar, 1212, Vila Clementino, S\u00e3o Paulo 04026-002, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1021\/np200906s","article-title":"Natural products as sources of new drugs over the 30 years from 1981 to 2010","volume":"75","author":"Newman","year":"2012","journal-title":"J. Nat. Prod."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5892","DOI":"10.1016\/j.bmc.2005.05.066","article-title":"Plant-based anticancer molecules: A chemical and biological profile of some important leads","volume":"13","author":"Srivastava","year":"2005","journal-title":"Bioorg. Med. 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