{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:05:07Z","timestamp":1791317107484,"version":"4.3.1"},"reference-count":24,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T00:00:00Z","timestamp":1397692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Traditional Chinese Medicine (TCM) nasal therapy has been utilized to treat numerous diseases for over two millennia. It has many advantages compared with other routes. In this article, headspace-solid phase microextraction-gas chromatography-mass spectrometry and high performance liquid chromatography-atmospheric pressure chemical ionization-ion trap-time of flight-multistage mass spectrometry were applied for the first time to analyze the absorbed constituents in rabbit plasma and cerebrospinal fluid (CSF) after intranasal administration of Asari Radix et Rhizoma (AR). In total, 47 absorbed AR constituents including 14 monoterpenes, 10 phenylpropanoids, four benzene derivatives, two alkanes, nine N-alkylamides and eight lignans were tentatively identified in the rabbit plasma and CSF. Thirty-three absorbed constituents are found to have different bioactivities related to the pharmacological actions of AR through bibliography data retrieval.  These indicated that many types of constituents of TCM can be absorbed at the nasal cavity into both rabbit blood and CSF. This is the first study to explore the absorption of AR, and comprehensively analyze the absorbed constituents after intranasal administration of TCM. These findings extend our understanding of the effective substances of AR, and inspire us to make a hypothesis on the mechanism of additive effect of multiple constituents of TCMs, which is very worthy of further investigation.<\/jats:p>","DOI":"10.3390\/molecules19044857","type":"journal-article","created":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T12:21:27Z","timestamp":1397737287000},"page":"4857-4879","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Identification of Absorbed Constituents in the Rabbit Plasma and Cerebrospinal Fluid after Intranasal Administration of Asari Radix et Rhizoma by HS-SPME-GC-MS and  HPLC-APCI-IT-TOF-MSn"],"prefix":"10.3390","volume":"19","author":[{"given":"Chen","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences,  Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences,  Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Mei","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences,  Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0088-7134","authenticated-orcid":false,"given":"Yu","family":"Jing","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences,  Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Ying","family":"Shang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences,  Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-Xue","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences,  Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences,  Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Qing","family":"Cai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences,  Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,4,17]]},"reference":[{"key":"ref_1","unstructured":"Gao, S.Z. (1994). Zhongyi Biliaofa Quanshu A Comprehensive Treatise on Nasal Therapy of Traditional Chinese Medicine, Huaxia Publishing House. [1st ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"288","DOI":"10.18433\/J3NC79","article-title":"Intranasal drug delivery: How, why and what for?","volume":"12","author":"Pires","year":"2009","journal-title":"J. Pharm. Pharm. Sci."},{"key":"ref_3","first-page":"88","article-title":"Overview on intranasal delivery of Chinese medicine in curing systemic diseases","volume":"4","author":"Yu","year":"2008","journal-title":"Asia Pac. Trad. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.ijpharm.2009.03.034","article-title":"Brain pharmacokinetics of tetramethylpyrazine after intranasal and intravenous administration in awake rats","volume":"375","author":"Feng","year":"2009","journal-title":"Int. J. Pharm."},{"key":"ref_5","first-page":"1871","article-title":"Study on pharmacokinetics of jasminoidin in rabbits administered Xingnaojing Naristillae by nasal medication","volume":"35","author":"Yao","year":"2010","journal-title":"Chin. J. Chin. Mater. Med."},{"key":"ref_6","first-page":"370","article-title":"Study on rat nasal administration with emulsion of Chinese herb Angelica Dahurica","volume":"10","author":"Gong","year":"2001","journal-title":"Chin. J. Clin. Pharm."},{"key":"ref_7","first-page":"211","article-title":"Exploration on nasal administration of traditional Chinese medicine for treating brain diseases","volume":"27","author":"Guo","year":"2011","journal-title":"J. Guangdong Pharm. Coll."},{"key":"ref_8","first-page":"132","article-title":"Study on the prescribing rules of traditional Chinese medicines for the treatment of migraine by intranasal administration","volume":"6","author":"Zhao","year":"2010","journal-title":"Asia Pac. Trad. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1002\/cbdv.200890033","article-title":"Constituents of Asarum sieboldii with inhibitory activity on lipopolysaccharide (LPS)-induced NO production in BV-2 microglial cells","volume":"5","author":"Han","year":"2008","journal-title":"Chem. Biodivers."},{"key":"ref_10","unstructured":"Wagner, H., Bauer, R., Melchart, D., Xiao, P.G., and Staudinger, A. (2011). Chromatographic Fingerprint Analysis of Herbal Medicines, Springer. [2nd ed.]."},{"key":"ref_11","first-page":"72","article-title":"Analgesic components of Asarum and its mechanisms","volume":"43","author":"Yuan","year":"2009","journal-title":"Shanghai J. Trad. Chin. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.jpba.2013.07.034","article-title":"Comparative analysis of two species of Asari Radix et Rhizoma by electronic nose, headspace GC-MS and chemometrics","volume":"85","author":"Li","year":"2013","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3613","DOI":"10.1002\/rcm.3243","article-title":"Electrospray ionization ion-trap time-of-flight tandem mass spectrometry of two furofurans: sesamin and gmelinol","volume":"21","author":"Yan","year":"2007","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3615","DOI":"10.1002\/rcm.3777","article-title":"Comparison of atmospheric pressure chemical ionization and electrospray ionization mass spectrometry for the detection of lignans from sesame seeds","volume":"22","author":"Struijs","year":"2008","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/jms.1276","article-title":"Identification of lignans by liquid chromatography-electrospray ionization ion-trap mass spectrometry","volume":"43","author":"Eklund","year":"2008","journal-title":"J. Mass Spectrom."},{"key":"ref_16","first-page":"198","article-title":"Rapid identification of Acanthopanax senticosus mixture by liquid chromatography-electrospray ion trap mass spectrometry","volume":"25","author":"Xing","year":"2004","journal-title":"J. Chin. Mass Spectrom. Soc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.1016\/j.bmcl.2012.01.136","article-title":"Anti-inflammatory and PPAR transactivational effects of secondary metabolites from the roots of Asarum sieboldii","volume":"22","author":"Quang","year":"2012","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.jpba.2010.02.010","article-title":"LC\u2013MS profiling of N-alkylamides in Spilanthes acmella extract and the transmucosal behaviour of its main bio-active spilanthol","volume":"53","author":"Boonen","year":"2010","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"12131","DOI":"10.1021\/jf303292t","article-title":"Seasonal variations in the concentrations of lipophilic compounds and phenolic acids in the roots of Echinacea purpurea and Echinacea pallida","volume":"60","author":"Thomsen","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"8086","DOI":"10.1021\/jf201158k","article-title":"Analysis of alkylamides in Echinacea plant materials and dietary supplements by ultrafast liquid chromatography with diode array and mass spectrometric detection","volume":"59","author":"Mudge","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.chroma.2005.11.008","article-title":"High performance liquid chromatography\/electrospray ionization mass spectrometry for simultaneous analysis of alkamides and caffeic acid derivatives from Echinacea purpurea extracts","volume":"1103","author":"Cech","year":"2006","journal-title":"J. Chromatogr. A"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1248\/cpb.29.564","article-title":"Structures of Amides from Asiasarum heterotropoides MAEK. var. mand shuricum MAEK","volume":"29","author":"Yasuda","year":"1981","journal-title":"Chem. Pharm. Bull."},{"key":"ref_23","first-page":"297","article-title":"Studies on chemical constituents from radix and rhizome of Asarum longerhizomatosum","volume":"33","author":"Zhang","year":"2002","journal-title":"Chin. Trad. Herba. Drugs"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0168-3659(02)00363-2","article-title":"Nasal drug delivery\u2014Possibilities, problems and solutions","volume":"87","author":"Illum","year":"2003","journal-title":"J. Control. 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