{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:24:03Z","timestamp":1782865443392,"version":"3.54.5"},"reference-count":36,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2014,6,23]],"date-time":"2014-06-23T00:00:00Z","timestamp":1403481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The currently available treatments for Chagas disease show limited therapeutic potential and are associated with serious side effects. Attempting to find alternative drugs isolated from Nature as agents against Trypanosoma cruzi has been our goal. Recently, we have demonstrated the in vitro anti-T. cruzi activities of two secondary metabolites isolated from the hydro-ethanolic extract of the aerial parts of Aristeguietia glutinosa (Lam.), (family Asteraceae). These active principles displayed poor hemolytic activity, low toxicity against murine macrophages, and absence of mutagenicity. Herein, proof of concept in vivo studies of the whole hydro-ethanolic extract of the aerial parts of Aristeguietia glutinosa and of the most active component isolated from the hydro-ethanolic extract, i.e., (+)-15-hydroxy-7-labden-17-al, was done in a murine acute model of Chagas disease. Both treatments caused a decrease in the animals\u2019 parasitemia. Metabolomic mechanism of action studies were done by 1H-NMR, both on the extract and on the active compounds, examining the effects of the metabolites both on membrane sterol biosynthesis and mitochondrial dehydrogenases, whereby we found that one of the metabolites inhibited the activity of the parasite mitochondrial dehydrogenases and the other inhibited the biosynthesis of parasite membrane sterols. The results are interesting in the context of popular use of plants for the treatment of Chagas disease.<\/jats:p>","DOI":"10.3390\/molecules19068488","type":"journal-article","created":{"date-parts":[[2014,6,24]],"date-time":"2014-06-24T02:39:58Z","timestamp":1403577598000},"page":"8488-8502","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["In Vivo Anti-Trypanosoma cruzi Activity of Hydro-Ethanolic Extract and Isolated Active Principles from Aristeguietia glutinosa and Mechanism of Action Studies"],"prefix":"10.3390","volume":"19","author":[{"given":"Javier","family":"Varela","sequence":"first","affiliation":[{"name":"Grupo de Qu\u00edmica Medicinal-Laboratorio de Qu\u00edmica Org\u00e1nica-Facultad de Ciencias-Facultad de Qu\u00edmica\u2014UdelaR, Igu\u00e1 4225, Montevideo, C.P. 11400, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elva","family":"Serna","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Tropical, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, Asunci\u00f3n, 1120, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Torres","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Tropical, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, Asunci\u00f3n, 1120, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"Yaluff","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Tropical, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, Asunci\u00f3n, 1120, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ninfa","family":"De Bilbao","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Tropical, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, Asunci\u00f3n, 1120, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricio","family":"Mi\u00f1o","sequence":"additional","affiliation":[{"name":"Carrera Qu\u00edmica Farmac\u00e9utica, Facultad de Ciencias Qu\u00edmicas, Universidad Central de Ecuador, Quito, C.P. 170150, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ximena","family":"Chiriboga","sequence":"additional","affiliation":[{"name":"Carrera Qu\u00edmica Farmac\u00e9utica, Facultad de Ciencias Qu\u00edmicas, Universidad Central de Ecuador, Quito, C.P. 170150, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1256-3786","authenticated-orcid":false,"given":"Hugo","family":"Cerecetto","sequence":"additional","affiliation":[{"name":"Grupo de Qu\u00edmica Medicinal-Laboratorio de Qu\u00edmica Org\u00e1nica-Facultad de Ciencias-Facultad de Qu\u00edmica\u2014UdelaR, Igu\u00e1 4225, Montevideo, C.P. 11400, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Grupo de Qu\u00edmica Medicinal-Laboratorio de Qu\u00edmica Org\u00e1nica-Facultad de Ciencias-Facultad de Qu\u00edmica\u2014UdelaR, Igu\u00e1 4225, Montevideo, C.P. 11400, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,6,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.4067\/S0716-97602010000300009","article-title":"Chagas disease: Present status of pathogenic mechanisms and chemotherapy","volume":"43","author":"Maya","year":"2010","journal-title":"Biol. 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