{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T03:10:09Z","timestamp":1789096209565,"version":"build-2803163510"},"reference-count":32,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T00:00:00Z","timestamp":1407196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Initial investigation for new active herbal extract with inhibiting activity on JAK\/STAT signaling pathway revealed that the extract of Caulis Trachelospermi, which was separated by 80% alcohol extraction and subsequent HP-20 macroporous resin column chromatography, was founded to strongly inhibit IFN-\u03b3-induced STAT1-responsive luciferase activity (IFN-\u03b3\/STAT1) with IC50 value of 2.43 \u03bcg\/mL as well as inhibiting IL-6-induced STAT3-responsive luciferase activity (IL-6\/STAT3) with IC50 value of 1.38 \u03bcg\/mL. Subsequent study on its active components led to the isolation and identification of two new dibenzylbutyrolactone lignans named 4-demethyltraxillaside (1) and nortrachelogenin 4-O-\u03b2-d-glucopyranoside (2), together with six known compounds. The lignan compounds 1\u20134 together with other lignan compounds isolated in previous study were tested the activities on IFN-\u03b3\/STAT1 and IL-6\/STAT3 pathways. The following result showed that the main components trachelogenin and arctigenin had corresponding activities on IFN-\u03b3\/STAT1 pathway with IC50 values of 3.14 \u03bcM and 9.46 \u03bcM as well as trachelogenin, arctigenin and matairesinol strongly inhibiting IL-6\/STAT3 pathway with IC50 values of 3.63 \u03bcM, 6.47 \u03bcM and 2.92 \u03bcM, respectively.<\/jats:p>","DOI":"10.3390\/molecules190811560","type":"journal-article","created":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T10:59:37Z","timestamp":1407236377000},"page":"11560-11571","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Active Components with Inhibitory Activities on IFN-\u03b3\/STAT1 and IL-6\/STAT3 Signaling Pathways from Caulis Trachelospermi"],"prefix":"10.3390","volume":"19","author":[{"given":"Xiao-Ting","family":"Liu","sequence":"first","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University,  Shenyang 110016, China"},{"name":"Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe-Xing","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University,  Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo-Feng","family":"Sun","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Min","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neng-Jiang","family":"Yu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,8,5]]},"reference":[{"key":"ref_1","unstructured":"Committee of Pharmacopoeia of the People\u2019s Republic of China (2010). 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