{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T09:40:16Z","timestamp":1777110016218,"version":"3.51.4"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T00:00:00Z","timestamp":1407196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"the Key Project of Scientific and Technological Innovation in Hangzhou","award":["No.20131813A08"],"award-info":[{"award-number":["No.20131813A08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study screened microRNAs (miRNAs) that are abnormally expressed in papillary thyroid carcinoma (PTC) tissues to identify PTC and nodular goiter and the degree of PTC malignancy. A total of 51 thyroid tumor tissue specimens paired with adjacent normal thyroid tissues were obtained from the Department of Surgical Oncology of Hangzhou First People\u2019s Hospital from June-December 2011. miRNA expression profiles were examined by microarrays and validated by quantitative real-time PCR (qRT-PCR). Expression levels of the miRNAs were analyzed to assess if they were associated with selected clinicopathological features. Eleven miRNAs were significantly differentially expressed between nodular goiter and PTC and between highly invasive and low invasive PTC. miR-199b-5p and miR-30a-3p were significantly differentially expressed among the three groups. miR-30a-3p, miR-122-5p, miR-136-5p, miR-146b-5p and miR-199b-5p were selected for further study by qRT-PCR and miR-146b-5p, miR-199b-5p and miR-30a-3p were different between the PTC and nodular goiter groups. miR-199b-5p was over-expressed in PTC patients with extrathyroidal invasion and cervical lymph node metastasis. In conclusion miR-146b-5p, miR-30a-3p, and miR-199b-5p may serve as biomarkers for the diagnosis of PTC and miR-199b-5p is associated with PTC invasiveness.<\/jats:p>","DOI":"10.3390\/molecules190811586","type":"journal-article","created":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T10:59:37Z","timestamp":1407236377000},"page":"11586-11599","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Expression Profile and Clinical Significance of MicroRNAs in Papillary Thyroid Carcinoma"],"prefix":"10.3390","volume":"19","author":[{"given":"You","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Oncological Surgery, Hangzhou First People's Hospital, Hangzhou 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Wuxi Second People's Hospital, Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ding-Cun","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Oncological Surgery, Wushan District of Hangzhou First People's Hospital, Hangzhou Cancer Hospital, Hangzhou 310002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Wang","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Oncological Surgery, Hangzhou First People's Hospital, Hangzhou 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oncological Surgery, Hangzhou First People's Hospital, Hangzhou 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Oncological Surgery, Hangzhou First People's Hospital, Hangzhou 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"277","DOI":"10.3322\/caac.20073","article-title":"Cancer statistics","volume":"60","author":"Jemal","year":"2010","journal-title":"CA Cancer J. 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